Construct: ORF TRCN0000465602
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001209.1_s317c1
- Derived from:
- ccsbBroadEn_02449
- DNA Barcode:
- ACCCGAAAACACTAATTGTTGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A17 (10478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465602
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NM_006358.4 | 100% | 100% | |
| 2 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NM_001282726.1 | 87.6% | 87.2% | 0_1ins112;2delT;4A>G |
| 3 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NM_001282727.1 | 76.2% | 76.2% | 114_115ins219 |
| 4 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NR_104237.1 | 35.3% | (many diffs) | |
| 5 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NR_104235.1 | 35.3% | (many diffs) | |
| 6 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NR_104236.1 | 33.5% | 1_151del;333_334ins152;921_2142del | |
| 7 | human | 10478 | SLC25A17 | solute carrier family 25 me... | NR_104238.1 | 33% | (many diffs) | |
| 8 | mouse | 20524 | Slc25a17 | solute carrier family 25 (m... | NM_011399.3 | 88.3% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttccgtgctg tcctacgaaa gcctggtcca cgccgtggcc ggagccgtgg 121 gaagcgtgac agcaatgaca gtgttttttc ccctggatac agctagactt cgacttcagg 181 ttgatgagaa aagaaaatcc aaaactacac acatggtgct cctggagatc attaaagaag 241 aaggactcct ggcaccatat cgagggtggt ttccagtgat ttccagtctc tgctgctcca 301 attttgtcta tttctacact tttaatagcc tcaaagcact ctgggtcaaa ggtcaacatt 361 ctaccactgg aaaagatctg gtagttgggt ttgttgcagg agtggttaat gtgttgctaa 421 caactccact ctgggtggta aacaccagac tgaagcttca aggagcaaaa tttaggaatg 481 aagacattgt accaacaaac tacaaaggta tcattgatgc ttttcatcag atcattcgcg 541 atgaaggaat ctcggcttta tggaatggca catttccctc attgctgttg gtcttcaatc 601 ctgccatcca gttcatgttt tatgaaggtt taaaacggca gcttttaaag aaacggatga 661 agctttcttc cttGGATGTG TTCATCATTG GTGCAGTAGC CAAAGCGATT GCCACCACGG 721 TGACCTATCC CCTGCAGACG GTACAGTCAA TTCTGAGGTT TGGGCGTCAT AGACTAAACC 781 CAGAAAACAG AACATTGGGA AGTCTTCGGA ATATTCTCTA TCTTCTTCAC CAACGAGTAA 841 GACGTTTTGG AATAATGGGA CTCTACAAAG GCCTTGAAGC CAAACTGCTG CAGACAGTCC 901 TCACTGCTGC TCTCATGTTC CTTGTTTATG AGAAACTGAC AGCTGCCACC TTCACAGTTA 961 TGGGGCTGAA GCGTGCACAC CAACACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1021 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1081 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCCGAAA 1141 ACACTAATTG TTGGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1201 aagatt