Construct: ORF TRCN0000465621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009959.1_s317c1
- Derived from:
- ccsbBroadEn_11653
- DNA Barcode:
- AACAAAACTCGTAACTTCCCCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPIA (22934)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22934 | RPIA | ribose 5-phosphate isomerase A | NM_144563.3 | 76.2% | 76.2% | 1_222del |
| 2 | mouse | 19895 | Rpia | ribose 5-phosphate isomerase A | NM_009075.2 | 70.7% | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaaa 61 ttggcatgtc caaggccgag gaggccaaga agctggcggg ccgcgcggct gtggagaacc 121 acgtgaggaa taaccaagtg ctgggaattg gaagtggttc tacaattgtc catgctgtgc 181 agcgaatagc tgaaagggtg aagcaagaga atctgaacct cgtctgtatt cccacttcct 241 tccaggcccg ccagctcatc ctgcagtatg gcttgaccct cagtgatctg gatcgacacc 301 cagagatcga ccttgccatc gatggtgctg atgaagtaga tgctgatctc aatctcatca 361 agggtggcgg aggctgcctg acccaggaga agattgtggc tGGCTATGCT AGTCGCTTCA 421 TCGTGATCGC TGATTTCAGG AAAGATTCGA AGAATCTCGG GGATCAGTGG CACAAGGGAA 481 TCCCCATCGA GGTCATCCCA ATGGCCTATG TCCCAGTGAG CCGAGCTGTG AGCCAGAAGT 541 TTGGGGGCGT GGTTGAACTT CGAATGGCTG TCAACAAGGC TGGTCCTGTG GTGACAGATA 601 ATGGGAATTT TATCTTGGAC TGGAAGTTTG ACCGGGTACA CAAATGGAGT GAAGTGAATA 661 CAGCTATCAA AATGATCCCA GGTGTGGTGG ACACAGGCCT ATTCATCAAC ATGGCTGAGA 721 GAGTCTACTT TGGGATGCAG GATGGCTCAG TGAACATGAG GGAGAAGCCT TTCTGTTGCC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAAACAAAAC TCGTAACTTC CCCATCACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt