Construct: ORF TRCN0000465624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011595.1_s317c1
- Derived from:
- ccsbBroadEn_13636
- DNA Barcode:
- GGTTGCTTGTGCATCGGCGCTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHFPL4 (375323)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465624
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375323 | LHFPL4 | LHFPL tetraspan subfamily m... | NM_198560.3 | 93.9% | 93.9% | 1_45del |
| 2 | human | 375323 | LHFPL4 | LHFPL tetraspan subfamily m... | XM_017006359.1 | 93.9% | 93.9% | 1_45del |
| 3 | human | 375323 | LHFPL4 | LHFPL tetraspan subfamily m... | XM_017006360.1 | 49.3% | 49.3% | (many diffs) |
| 4 | mouse | 269788 | Lhfpl4 | lipoma HMGIC fusion partner... | NM_177763.3 | 88.2% | 92.3% | (many diffs) |
| 5 | mouse | 269788 | Lhfpl4 | lipoma HMGIC fusion partner... | XM_017321600.1 | 88.2% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 765
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggaactcg cgggccatcg gcgtgctgtg ggccatcttc accatctgct 121 tcgccatcat caacgtggtg gtcttcatcc agccctactg ggtgggcgac agcgtgagca 181 cccccaagcc tggctacttc ggcctcttcc actactgcgt gggcagcggg ctggcgggcc 241 gcgagctcac ctgccggggc tccttcaccg acttcagcac catcccgtcc agcgccttca 301 aggcggccgc cttcttcgtg ctgctctcca tggtgctgat cctcggctgc atcacctgct 361 tttcgctttt cttcttctgc aacacggcta cggtctacaa gatctgcgcc tggatgcagc 421 tcttggcagc tctgtgcctc gtcctgggct gcatgatctt tccTGATGGC TGGGATGCCG 481 AGACCATCCG GGACATGTGT GGGGCCAAGA CGGGGAAGTA CTCCCTGGGG GACTGTTCAG 541 TGCGCTGGGC ATACATCCTG GCCATCATCG GCATCCTCAA CGCCCTCATC CTCTCCTTCC 601 TCGCCTTCGT GCTGGGCAAC CGGCAAACAG ACCTGCTGCA GGAGGAGCTC AAGCCGGAGA 661 ACAAAGATTT TGTGGGCTCT ACAGTAAGCT CCGTGTTGCG GCCCGGGGGT GATGTCTCTG 721 GATGGGGAGT CCTTCCCTGC CCCGTGGCTC ACTCACAGGG ACCCTTGCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA GGTTGCTTGT GCATCGGCGC TTCTACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt