Construct: ORF TRCN0000465634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001871.1_s317c1
- Derived from:
- ccsbBroadEn_13194
- DNA Barcode:
- AGCACCTTTTTCTGGCCAACCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGCZ (137868)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 137868 | SGCZ | sarcoglycan zeta | NM_001322879.2 | 95.3% | 95.3% | 1_39del |
| 2 | human | 137868 | SGCZ | sarcoglycan zeta | NM_139167.4 | 84.9% | 84.9% | 1_39del;234_335del |
| 3 | human | 137868 | SGCZ | sarcoglycan zeta | NM_001322880.2 | 71.7% | 71.7% | 1_39del;234_335del;422_423ins123 |
| 4 | human | 137868 | SGCZ | sarcoglycan zeta | NM_001322881.2 | 66.3% | 64.5% | (many diffs) |
| 5 | mouse | 244431 | Sgcz | sarcoglycan zeta | XM_011242138.1 | 79.1% | 84.2% | (many diffs) |
| 6 | mouse | 244431 | Sgcz | sarcoglycan zeta | NM_145841.2 | 75.8% | 80.7% | (many diffs) |
| 7 | mouse | 244431 | Sgcz | sarcoglycan zeta | XM_006509120.3 | 75.8% | 80.7% | (many diffs) |
| 8 | mouse | 244431 | Sgcz | sarcoglycan zeta | XM_006509122.1 | 71% | 75.4% | (many diffs) |
| 9 | mouse | 244431 | Sgcz | sarcoglycan zeta | XM_011242139.2 | 30.5% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 864
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacacgagaa caatacatac tagcaaccca acagaataac ctgccaagga 121 ctgagaatgc acaactttac ccagtgggaa tttatggatg gcgaaagagg tgcttatact 181 tctttgtcct tctgctgttg gttaccatga tagttaactt agccatgaca atatggatat 241 tgaaagttat gaatttcact gtggatagtc cgctggtctt acagtctgac aggaatgtca 301 cagtgaatgc aagaaatcac atggggcagt taaccggaca gctgaccata ggagctgatg 361 ctgtggaagc tcagtgtaaa agatttgaag tgagagccag tgaagatggc agggtgctgt 421 tttctgcaga tgaagatgag attaccattg gggctgaaaa gctgaaagtt acaggcactg 481 aaggagccgt atttgggcac tctgtggaga cgccgcacat cagagcagag ccatcccaag 541 atctcaggct tgaatcaccc accagatcct tgatcatgga agctcCCCGT GGGGTCCAGG 601 TGAGTGCTGC TGCAGGAGAC TTCAAGGCCA CCTGCAGGAA GGAGCTCCAT CTGCAATCTA 661 CAGAAGGGGA GATATTTTTA AATGCAGAGA CAATCAAGCT GGGAAATCTA CCAACTGGCT 721 CCTTCTCATC TTCTTCACCC AGCTCCTCAA GTTCTCGACA GACAGTGTAT GAACTCTGCG 781 TCTGCCCCAA TGGCAAACTT TACCTTTCTC CAGCAGGAGT AGGTTCCACT TGTCAGTCCA 841 GTAGCAACAT CTGCCTGTGG AGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCACCTTTTT 1021 CTGGCCAACC AGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att