Construct: ORF TRCN0000465665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013144.1_s317c1
- Derived from:
- ccsbBroadEn_12801
- DNA Barcode:
- AAACGACCTCCGCTAAAGCCTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTBD10 (84280)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465665
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84280 | BTBD10 | BTB domain containing 10 | NM_001297741.1 | 43% | 43% | 1_729del |
| 2 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_017018411.1 | 43% | 43% | 1_729del |
| 3 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_017018409.1 | 39.8% | 39.8% | 1_729del;863_967del |
| 4 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_017018410.1 | 39.8% | 39.8% | 1_729del;863_967del |
| 5 | human | 84280 | BTBD10 | BTB domain containing 10 | NM_032320.6 | 38.7% | 38.7% | 1_873del |
| 6 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_024448713.1 | 38.7% | 38.7% | 1_873del |
| 7 | human | 84280 | BTBD10 | BTB domain containing 10 | NM_001297742.1 | 38% | 38% | 1_897del |
| 8 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_017018406.2 | 36% | 36% | 1_873del;1007_1111del |
| 9 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_024448712.1 | 36% | 36% | 1_873del;1007_1111del |
| 10 | human | 84280 | BTBD10 | BTB domain containing 10 | XM_017018405.1 | 35.5% | 35.5% | 1_897del;1031_1135del |
| 11 | human | 84280 | BTBD10 | BTB domain containing 10 | XR_001748002.2 | 22.6% | 1_973del;1107_1202del;1622_2435del | |
| 12 | human | 84280 | BTBD10 | BTB domain containing 10 | XR_001748001.2 | 21.3% | 1_1118del;1252_1347del;1767_2580del | |
| 13 | human | 84280 | BTBD10 | BTB domain containing 10 | XR_001748000.2 | 15.3% | 1_2138del;2272_2367del;2787_3600del | |
| 14 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | XM_006508161.2 | 39.8% | 42.3% | (many diffs) |
| 15 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | XM_006508162.2 | 39.8% | 42.3% | (many diffs) |
| 16 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | XM_006508163.2 | 39.8% | 42.3% | (many diffs) |
| 17 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | NM_133700.2 | 35.8% | 38.1% | (many diffs) |
| 18 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | XM_006508160.2 | 35.8% | 38.1% | (many diffs) |
| 19 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | XM_011241890.1 | 35.8% | 38.1% | (many diffs) |
| 20 | mouse | 68815 | Btbd10 | BTB (POZ) domain containing 10 | XM_006508159.3 | 35.2% | 37.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cctccctctc atggtagcta gtgcccagag tggggaacgg gaatgtcata 121 tagtggtgct tacagatgat gatgtggttg attgggatga agaatatcca ccacagatgg 181 gagaagaata ttcacaaatt atttatagca caaaattata tagatttttc aagtatattg 241 aaaacagaga tgtggccaag tcagttttga aggagagggg tcttaagaag attagattgg 301 gaatagaagg ttatCCTACC TACAAAGAAA AAGTAAAGAA AAGGCCTGGA GGCCGCCCAG 361 AAGTGATCTA CAACTATGTC CAAAGACCCT TTATTCGAAT GTCCTGGGAG AAGGAAGAAG 421 GAAAGAGTCG GCATGTAGAC TTTCAGTGTG TAAAGAGTAA ATCTATCACC AATCTTGCAG 481 CAGCTGCAGC AGACATTCCC CAGGACCAGC TGGTAGTCAT GCATCCAACT CCACAAGTGG 541 ATGAGCTGGA TATTCTCCCT ATCCATCCCC CTTCTGGCAA CAGTGACCTC GATCCTGATG 601 CACAGAATCC AATGCTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAACGAC CTCCGCTAAA 781 GCCTCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt