Transcript: Human XR_001748002.2

PREDICTED: Homo sapiens BTB domain containing 10 (BTBD10), transcript variant X10, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
BTBD10 (84280)
Length:
2435
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001748002.2
NBCI Gene record:
BTBD10 (84280)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001748002.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413002 GAGCGATTCTGGATTACTATA pLKO_005 777 3UTR 100% 13.200 18.480 N BTBD10 n/a
2 TRCN0000168331 GAAGGTTATCCTACCTACAAA pLKO.1 1310 3UTR 100% 5.625 7.875 N BTBD10 n/a
3 TRCN0000421015 GTATTCCTCCAGTACAATTTG pLKO_005 2088 3UTR 100% 13.200 9.240 N BTBD10 n/a
4 TRCN0000413953 TATAGTTGTGATGCACAATAT pLKO_005 2046 3UTR 100% 13.200 9.240 N BTBD10 n/a
5 TRCN0000426826 ACGAGATTCATCTCATGAAAG pLKO_005 313 3UTR 100% 10.800 7.560 N BTBD10 n/a
6 TRCN0000168451 GAAACAGTAGTCAGTCAAGTT pLKO.1 495 3UTR 100% 4.950 3.465 N BTBD10 n/a
7 TRCN0000168717 GAGTGACACTAATAGTGGATA pLKO.1 603 3UTR 100% 4.950 3.465 N BTBD10 n/a
8 TRCN0000167507 GCACATAACAATGTTTAGGAT pLKO.1 1722 3UTR 100% 3.000 2.100 N BTBD10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001748002.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12801 pDONR223 100% 22.6% None 1_973del;1107_1202del;1622_2435del n/a
2 ccsbBroad304_12801 pLX_304 0% 22.6% V5 1_973del;1107_1202del;1622_2435del n/a
3 TRCN0000465665 AAACGACCTCCGCTAAAGCCTCCC pLX_317 54.8% 22.6% V5 1_973del;1107_1202del;1622_2435del n/a
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