Construct: ORF TRCN0000465680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005531.2_s317c1
- Derived from:
- ccsbBroadEn_14809
- DNA Barcode:
- AGGTGACCTATTCTTTCAGAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K5 (5607)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5607 | MAP2K5 | mitogen-activated protein k... | NM_145160.3 | 100% | 100% | |
| 2 | human | 5607 | MAP2K5 | mitogen-activated protein k... | NM_002757.4 | 97.7% | 97.7% | 1042_1043ins30 |
| 3 | human | 5607 | MAP2K5 | mitogen-activated protein k... | NM_001206804.2 | 91.3% | 90.2% | (many diffs) |
| 4 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_024449988.1 | 82.8% | 82.8% | 0_1ins231 |
| 5 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_011521787.3 | 64.2% | 63.6% | (many diffs) |
| 6 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_011521788.3 | 63% | 62.9% | 848_849delGGins497 |
| 7 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_017022414.2 | 59.3% | 59.3% | 797_798delCA;801_802ins545 |
| 8 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XR_001751355.2 | 48.6% | 1_651del;1449_1450ins49;1621_1622ins325 | |
| 9 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | NM_011840.2 | 91% | 97.7% | (many diffs) |
| 10 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_006511145.2 | 89% | 95.7% | (many diffs) |
| 11 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_006511146.2 | 88.8% | 95.5% | (many diffs) |
| 12 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_011242740.2 | 85.3% | 91% | (many diffs) |
| 13 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_011242741.2 | 72.7% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1410
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtggctagcc cttggcccct ttcctgccat ggagaaccag gtgctggtaa 121 ttcgcatcaa gatcccaaat agtggcgcgg tggactggac agtgcactcc gggccgcagt 181 tactcttcag ggatgtgctg gatgtgatag gccaggttct gcctgaagca acaactacag 241 catttgaata tgaagatgaa gatggtgatc gaattacagt gagaagtgat gaggaaatga 301 aggcaatgct gtcatattat tattccacag taatggaaca gcaagtaaat ggacagttaa 361 tagagcctct gcagatattt ccaagagcct gcaagcctcc tggggaacgg aacatacatg 421 gcctgaaggt gaatactcgg gccggaccct ctcaacacag cagcccagca gtctcagatt 481 cacttccaag caatagctta aagaagtctt ctgctgaact gaaaaaaata ctagccaatg 541 gccagatgaa tgaacaagac atacgatatc gggacactct tggtcatggc aacggaggca 601 cagtctacaa agcatatcat gtcccgagtg ggaaaatatt agctgtaaag gtcatactac 661 tagatattac actggaactt cagaagcaaa ttatgtctga attggaaatt ctttataagt 721 gcgattcatc atatatcatt ggattttatg gagcattttt tgtagaaaac aggatttcaa 781 tatgtacaga attcatggat gggggatctt tggatgtata taggaaaatg ccagaacatg 841 tccttggaag aattgcagta gcagttgtta aaggccttac ttatttgtgg agtttaaaga 901 ttttacatag agacgtgaag ccctccaata tgctagtaaa cacaagagga caggttaagc 961 tgtgtgattt tggagttagc actcagctgg tgaattctat agccaagacg tatgttggaa 1021 caaatgctta tatggcgcct gaaaggattt caggggagca gtatggaatt cattctgatg 1081 tctggagctt aggaatctct tttatggagc ttgctcttgg gaggtttcca tatccTCAGA 1141 TTCAGAAAAA CCAGGGATCT TTAATGCCTC TCCAGCTTCT GCAGTGCATT GTTGATGAGG 1201 ATTCGCCCGT CCTTCCAGTT GGAGAGTTCT CGGAGCCATT TGTACATTTC ATCACTCAGT 1261 GTATGCGAAA ACAGCCAAAA GAAAGGCCAG CACCTGAAGA ATTGATGGGC CACCCGTTCA 1321 TCGTGCAGTT CAATGATGGA AATGCCGCCG TGGTGTCCAT GTGGGTGTGC CGGGCGCTGG 1381 AGGAGAGGCG GAGCCAGCAG GGGCCCCCGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1441 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1501 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGGTG 1561 ACCTATTCTT TCAGAAATCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1621 tgaaagatt