Construct: ORF TRCN0000465695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003132.1_s317c1
- Derived from:
- ccsbBroadEn_08735
- DNA Barcode:
- GACTAAAGGTCGTCCTACCGATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAMD1B (57476)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_020716.3 | 99.9% | 100% | 1425T>C |
| 2 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018044.1 | 99.4% | 99.4% | 1425T>C;1889_1890insGATCTGTTTCAG |
| 3 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001286563.2 | 99% | 99% | 235_255del;1446T>C |
| 4 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018043.1 | 96.5% | 96.1% | (many diffs) |
| 5 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001367419.1 | 96.1% | 95.4% | (many diffs) |
| 6 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001367418.1 | 95.6% | 94.9% | (many diffs) |
| 7 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001286564.2 | 94.5% | 94.5% | 0_1ins120;1305T>C |
| 8 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018047.1 | 94.5% | 94.5% | 0_1ins120;1305T>C |
| 9 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018048.1 | 94.5% | 94.5% | 0_1ins120;1305T>C |
| 10 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_005271619.4 | 94.3% | 94% | (many diffs) |
| 11 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001330396.2 | 93.9% | 94% | 0_1ins120;1305T>C;1769_1770insGATCTGTTTCAG |
| 12 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018050.1 | 93.9% | 94% | 0_1ins120;1305T>C;1769_1770insGATCTGTTTCAG |
| 13 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_011542929.2 | 93.8% | 93.1% | (many diffs) |
| 14 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_024448622.1 | 93.6% | 93% | (many diffs) |
| 15 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001367420.1 | 93.2% | 92.5% | (many diffs) |
| 16 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_011542933.1 | 92.7% | 87.4% | (many diffs) |
| 17 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018040.1 | 91.7% | 91.2% | (many diffs) |
| 18 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_005271624.4 | 91.7% | 90.9% | (many diffs) |
| 19 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018039.1 | 90.9% | 89.7% | (many diffs) |
| 20 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_011542926.2 | 89.2% | 88.1% | (many diffs) |
| 21 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_005271621.4 | 89.1% | 88.7% | (many diffs) |
| 22 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_005271623.4 | 89.1% | 88.7% | (many diffs) |
| 23 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_011542930.2 | 89.1% | 88.7% | (many diffs) |
| 24 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_011542931.2 | 89.1% | 88.7% | (many diffs) |
| 25 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018045.1 | 89.1% | 88.7% | (many diffs) |
| 26 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018046.1 | 89.1% | 88.7% | (many diffs) |
| 27 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_006718892.3 | 89.1% | 88% | (many diffs) |
| 28 | human | 57476 | GRAMD1B | GRAM domain containing 1B | NM_001367421.2 | 87.4% | 86.7% | (many diffs) |
| 29 | human | 57476 | GRAMD1B | GRAM domain containing 1B | XM_017018051.1 | 78.5% | 78.5% | 0_1ins474;951T>C |
| 30 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510247.3 | 90.9% | 97.2% | (many diffs) |
| 31 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313327.1 | 90.3% | 96.7% | (many diffs) |
| 32 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510246.3 | 89.6% | 95.4% | (many diffs) |
| 33 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510245.3 | 89.1% | 95.3% | (many diffs) |
| 34 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313328.1 | 89% | 94.9% | (many diffs) |
| 35 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313326.1 | 88.5% | 94.8% | (many diffs) |
| 36 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510243.3 | 87.8% | 93.5% | (many diffs) |
| 37 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510242.3 | 87.4% | 92.3% | (many diffs) |
| 38 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510244.3 | 87% | 92.2% | (many diffs) |
| 39 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313325.1 | 85.8% | 91% | (many diffs) |
| 40 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510251.3 | 85.7% | 91.8% | (many diffs) |
| 41 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510252.3 | 85.7% | 91.8% | (many diffs) |
| 42 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510250.3 | 84.1% | 84.2% | (many diffs) |
| 43 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510248.3 | 84% | 90% | (many diffs) |
| 44 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510249.3 | 84% | 90% | (many diffs) |
| 45 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313329.1 | 83.5% | 83.7% | (many diffs) |
| 46 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510237.3 | 75.9% | 80.4% | (many diffs) |
| 47 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | NM_172768.1 | 75.4% | 80% | (many diffs) |
| 48 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510236.3 | 75.3% | 79.8% | (many diffs) |
| 49 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_006510235.3 | 74.7% | 79.2% | (many diffs) |
| 50 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313319.1 | 74.6% | 79.1% | (many diffs) |
| 51 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313320.1 | 74.6% | 79.1% | (many diffs) |
| 52 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313321.1 | 74.6% | 79.1% | (many diffs) |
| 53 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313322.1 | 74.6% | 79.1% | (many diffs) |
| 54 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313323.1 | 74.2% | 78.7% | (many diffs) |
| 55 | mouse | 235283 | Gramd1b | GRAM domain containing 1B | XM_017313324.1 | 74.2% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2283
- ORF length:
- 2214
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaggattc aagctctcct gcactgccag taactccaac cgcagcacgc 121 cggcctgctc gcccatcctc cggaagcggt ctcgctcgcc aaccccgcag aaccaggacg 181 gagacaccat ggtggagaag ggctcagatc actcctcgga caagtccccg tccacaccgg 241 agcagggcgt gcagcgcagc tgctcctccc agtccggccg gagcggcggc aagaattcca 301 agaaaagcca gagttggtat aatgtgttaa gccccaccta caagcagaga aatgaagact 361 tcagaaagct ctttaagcag cttccagaca cggagcgcct cattgttgat tactcatgtg 421 cactccaaag agacattctc cttcagggcc gactctacct ctctgaaaat tggatctgct 481 tctacagcaa catcttccgc tgggaaactc tgctgacagt ccgtttgaaa gacatctgtt 541 ccatgactaa agaaaaaaca gctcgcctca ttcccaatgc catccaagtt tgcactgatt 601 cagaaaagca cttcttcact tcgtttgggg cccgggatag gacatatatg atgatgttcc 661 ggctctggca gaatgctctc cttgaaaagc ctctgtgtcc caaggagctc tggcactttg 721 ttcaccagtg ctatgggaac gaattgggcc tgaccagtga tgacgaggac tacgtgcccc 781 ctgacgacga cttcaacaca atgggatact gtgaagagat ccctgtggaa gagaatgaag 841 tgaatgacag ctcatccaag agcagcatag agaccaagcc agatgccagt ccacagctgc 901 ccaagaaatc catcaccaac agcacactaa catccacagg gagcagtgag gcccccgtct 961 cgtttgatgg gctgcccctg gaggaagagg cgctggaggg agacgggtcc ctggaaaagg 1021 agctcgccat tgacaacatc atgggggaga agattgagat gatcgctcct gtgaactccc 1081 cttcactgga cttcaatgac aatgaggaca tccccactga gctcagtgac tcttccgaca 1141 cacacgatga aggagaggtc caggccttct atgaggacct gagtggccgg cagtacgtga 1201 atgaagtctt caacttcagc gtggacaagc tctatgacct cctcttcacc aactcgccct 1261 tccagcggga tttcatggag cagcggcgct tctctgatat catcttccat ccatggaaaa 1321 aggaggagaa tggaaaccag agccgagtga ttctttacac catcaccctt accaaccctc 1381 tggctcccaa aactgccact gtcagggaga cacagaccat gtacaaggcg agccaggaga 1441 gtgaatgtta cgtgatagat gccgaagtcc tcacccacga cgtgccctac cacgactact 1501 tctacacaat caatcgctac acgctcaccc gtgtggctcg gaacaagagc cgactcaggg 1561 tctccacaga gctgcgctat cgaaaacagc cctgggggtt agtgaaaacg ttcatcgaga 1621 agaacttctg gagtgggctg gaggactact tccgccattt agagagcgag ctggccaaaa 1681 cggagagcac ttatttggct gagatgcaca gacaatctcc caaagagaag gccagcaaga 1741 ctacaacggt gcggaggagg aagcgtcccc atgcccacct gcgagtccct cacctggaag 1801 aggtgatgag cccggtcacc acgcccacag atgaggatgt gggccacagg atcaaacatg 1861 tggcaggttc cacacagacg cggcatatcc cggaggacac ccccaacggt ttccacctgc 1921 agagcgtgtc CAAGCTGCTG CTGGTTATCA GCTGTGTGAT CTGTTTCAGT CTGGTGCTGC 1981 TGGTCATCCT TAACATGATG CTCTTCTACA AACTCTGGAT GTTGGAATAC ACCACGCAGA 2041 CCCTCACTGC CTGGCAGGGT CTAAGGCTCC AAGAAAGGTT ACCCCAGTCT CAGACAGAAT 2101 GGGCCCAGCT CTTAGAGTCC CAACAAAAGT ACCACGATAC TGAGCTCCAA AAATGGAGGG 2161 AAATCATCAA ATCCTCAGTG ATGCTCCTTG ACCAGATGAA GGACTCGCTC ATCAACCTTC 2221 AGAACGGCAT CAGGTCCCGC GACTACACGT CGGAAAGTGA AGAAAAGAGG AATCGCTATC 2281 ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2341 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2401 GGCTTTATAT ATCTTGTGGA AAGGACGAGA CTAAAGGTCG TCCTACCGAT TAACGCGTTA 2461 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt