Construct: ORF TRCN0000465699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003544.1_s317c1
- Derived from:
- ccsbBroadEn_11978
- DNA Barcode:
- CAGTTAAAACCCGCCCAATTCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRR16 (51334)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51334 | PRR16 | proline rich 16 | NM_001308087.1 | 99.8% | 99.5% | 118C>A |
| 2 | human | 51334 | PRR16 | proline rich 16 | NM_001308088.1 | 99.8% | 99.5% | 118C>A |
| 3 | human | 51334 | PRR16 | proline rich 16 | XM_011543454.2 | 99.7% | 99.5% | 118C>A;120G>A |
| 4 | human | 51334 | PRR16 | proline rich 16 | XM_011543452.2 | 91.5% | 91.3% | 1_63del;181C>A;183G>A |
| 5 | human | 51334 | PRR16 | proline rich 16 | XM_011543453.3 | 91.5% | 91.3% | 1_63del;181C>A;183G>A |
| 6 | human | 51334 | PRR16 | proline rich 16 | NM_016644.2 | 83.1% | 82.9% | 1_141del;259C>A |
| 7 | human | 51334 | PRR16 | proline rich 16 | NM_001300783.2 | 76.7% | 76.6% | 1_210del;328C>A;330G>A |
| 8 | human | 51334 | PRR16 | proline rich 16 | XR_001742094.1 | 12.4% | (many diffs) | |
| 9 | human | 51334 | PRR16 | proline rich 16 | XR_001742093.1 | 11.7% | (many diffs) | |
| 10 | mouse | 71373 | Prr16 | proline rich 16 | NM_001081224.2 | 67.6% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgacagctcc aaaacggaca cgctgaatag tagctcaagt ggcacaacag 121 cctccagcct agagaagatc aaagtgcagg ctaatgcacc gcttattaaa cccccagcac 181 acacatctgc tatcctcacg gtcctgagaa agccaaaccc tccaccacct cctccaaggt 241 tgacacctgt gaagtgtgaa gaccccaaaa gggtggttcc aactgccaat cctgtaaaaa 301 ccaatggcac ccttctacga aatggaggct taccaggtgg acctaacaaa attccaaatg 361 gagatatctg ctgcataccc aacagtaact tggacaaggc tccagtccag cttctgatgc 421 atagacctga aaaagacaga tgtccccagg cagggcctcg agaacgagtt cggtttaatg 481 aaaaagtaca gtaccatggc tattgtcctg actgtgatac ccggtataac ataaaaaaca 541 gggaggtcca cttacacagt gaacctgtcc acccaccggg aaagattcct caccaaggcc 601 ctccCCTCCC TCCTACACCC CATCTCCCTC CTTTCCCACT AGAAAATGGG GGAATGGGAA 661 TAAGCCACAG TAACAGCTTC CCCCCTATCA GACCTGCAAC TGTGCCTCCT CCCACTGCAC 721 CAAAACCACA GAAGACGATC TTGAGGAAGT CAACCACTAC AACCGTGTGC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGACAGTTAA AACCCGCCCA ATTCCAAACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt