Construct: ORF TRCN0000465721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017797.1_s317c1
- Derived from:
- ccsbBroadEn_01434
- DNA Barcode:
- TGTTGTTCGTTATACTAAATGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL32 (6161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6161 | RPL32 | ribosomal protein L32 | NM_000994.4 | 100% | 100% | |
| 2 | human | 6161 | RPL32 | ribosomal protein L32 | NM_001007073.1 | 100% | 100% | |
| 3 | human | 6161 | RPL32 | ribosomal protein L32 | NM_001007074.1 | 100% | 100% | |
| 4 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NR_125355.2 | 10.7% | (many diffs) | |
| 5 | mouse | 19951 | Rpl32 | ribosomal protein L32 | NM_172086.2 | 91.3% | 100% | (many diffs) |
| 6 | mouse | 621697 | Rpl32l | ribosomal protein L32-like | NM_001101561.2 | 87.4% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcccTCAGA CCCCTTGTGA AGCCCAAGAT CGTCAAAAAG AGAACCAAGA 121 AGTTCATCCG GCACCAGTCA GACCGATATG TCAAAATTAA GCGTAACTGG CGGAAACCCA 181 GAGGCATTGA CAACAGGGTT CGTAGAAGAT TCAAGGGCCA GATCTTGATG CCCAACATTG 241 GTTATGGAAG CAACAAAAAA ACAAAGCACA TGCTGCCCAG TGGCTTCCGG AAGTTCCTGG 301 TCCACAACGT CAAGGAGCTG GAAGTGCTGC TGATGTGCAA CAAATCTTAC TGTGCCGAGA 361 TCGCTCACAA TGTTTCCTCC AAGAACCGCA AAGCCATCGT GGAAAGAGCT GCCCAACTGG 421 CCATCAGAGT CACCAACCCC AATGCCAGGC TGCGCAGTGA AGAAAATGAG TACCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGATGTT GTTCGTTATA CTAAATGTCT ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt