Construct: ORF TRCN0000465722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006091.1_s317c1
- Derived from:
- ccsbBroadEn_12334
- DNA Barcode:
- GGGTTTTAACGACCCCGTAGCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD50 (57182)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465722
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57182 | ANKRD50 | ankyrin repeat domain 50 | NM_001167882.2 | 59.4% | 59.4% | 1_1521del |
| 2 | human | 57182 | ANKRD50 | ankyrin repeat domain 50 | NM_020337.3 | 51.9% | 51.9% | 1_2058del |
| 3 | human | 57182 | ANKRD50 | ankyrin repeat domain 50 | XM_017008471.1 | 51.9% | 51.9% | 1_2058del |
| 4 | mouse | 99696 | Ankrd50 | ankyrin repeat domain 50 | XM_006535582.3 | 42% | 48.8% | (many diffs) |
| 5 | mouse | 99696 | Ankrd50 | ankyrin repeat domain 50 | XM_011249725.2 | 42% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2295
- ORF length:
- 2229
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg acatagagag attgtggaac acctactgga ccatggagca gaagtaaatc 121 atgaggatgt tgatggcagg actgcactct ctgtagctgc actttgtgtg cctgcaagta 181 aagggcacgc atcagttgtt agccttttaa ttgatcgagg tgctgaagta gatcattgtg 241 ataaagatgg catgactcca ctgctggtag ctgcctatga aggacatgtt gatgtggttg 301 acttgcttct agaaggggga gcagatgtag atcacacaga taacaatggc cgtacacccc 361 tcttagcagc agcgtctatg ggtcatgcat cagttgtaaa tacacttttg ttttggggtg 421 cagctgtgga tagtattgat agtgaaggta ggacagtcct cagtatagct tcagcacaag 481 gaaatgttga ggtggtacgt actctactgg atagagggtt agatgaaaat cacagagatg 541 atgctggatg gacacctttg cacatggcag cttttgaagg gcacagattg atatgtgaag 601 cacttattga acaaggtgct agaacaaatg agattgacaa tgatggacga atccctttca 661 tattagcttc acaagagggt cattatgatt gtgttcaaat attactggaa aacaaatcca 721 acattgatca aagaggttat gatggaagaa atgcactgcg ggttgctgca ttagaagggc 781 acagggacat tgttgaattg ctttttagcc atggtgctga tgttaactgc aaagatgctg 841 atggtcggcc tacactttat atcttggcct tagaaaatca gcttacaatg gccgaatatt 901 ttttagaaaa tggtgcaaac gtagaagcaa gtgatgctga aggaaggaca gcacttcatg 961 tgtcttgttg gcaaggccat atggaaatgg tgcaggtcct gatagcatac catgctgacg 1021 tcaatgctgc agacaatgaa aagcgctctg ctttgcagtc tgcagcctgg cagggccatg 1081 taaaagtggt tcagcttctg attgagcatg gtgctgtagt tgaccataca tgtaaccaag 1141 gtgcaactgc actctgtatt gcagcccagg aagggcacat tgatgttgtt caggtcttat 1201 tagagcatgg tgctgatcca aaccatgctg atcaatttgg acgcactgct atgcgtgttg 1261 cagccaaaaa tggacattct cagataatta aattattaga aaaatatggt gcatctagtt 1321 tgaatggctg ttccccatct cctgttcaca caatggagca aaaacctcta cagtcattgt 1381 cttcaaaagt gcagtcatta acaattaaat caaatagctc tggtagtact ggtggagggg 1441 atatgcagcc ttcgttacgt ggtttaccta atgggcctac tcatgctttt agttctcctt 1501 cagaatctcc agattctaca gttgaccggc agaagtcatc actgtcaaat aattccctga 1561 aaagctcaaa aaattcatct ttgagaacta cttcatctac agcaacggct caaacagtgc 1621 caattgatag ctttcataac ttgtcattta cagaacaaat tcagcagcat tcattgccac 1681 gcagtagaag tcgacagtca attgtttccc catcttccac aacacagtcc ttaggacaga 1741 gtcataattc accaagtagt gaatttgagt ggagtcaagt aaagcccagt ttgaagtcaa 1801 ctaaagcaag taaagggggg aaatcagaaa attctgccaa gtctggatca gctgggaaaa 1861 aagcgaaaca aagtaattct tcacagccaa aggtttTAGA ATATGAAATG ACTCAGTTTG 1921 ATAGAAGAGG ACCTATAGCC AAATCCGGGA CTGCTGCACC ACCTAAACAA ATGCCAGCAG 1981 AATCTCAATG CAAAATTATG ATACCTTCAG CTCAGCAGGA AATTGGTCGA TCTCAACAGC 2041 AGTTTCTTAT TCACCAACAA AGTGGGGAAC AGAAGAAGAG AAATGGAATA ATGACAAATC 2101 CAAATTATCA TCTTCAGAGC AACCAGGTTT TTCTTGGTAG GGTTTCAGTC CCACGAACAA 2161 TGCAAGATAG AGGGCATCAG GAAGTGTTGG AGGGATACCC TTCCTCAGAG ACAGAATTAA 2221 GCCTTAAACA AGCTCTGAAG CTTCAGATTG AAGGTTCTGA CCCTAGCTTC AACTATAAAA 2281 AGGAAACACC ATTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGGTTTTAAC GACCCCGTAG 2461 CAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt