Construct: ORF TRCN0000465729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017319.1_s317c1
- Derived from:
- ccsbBroadEn_07890
- DNA Barcode:
- AAACCAAGTACTCCTGGCATAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBX7 (23492)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23492 | CBX7 | chromobox 7 | NM_175709.5 | 99.7% | 99.2% | 230A>G;715G>C |
| 2 | human | 23492 | CBX7 | chromobox 7 | NM_001346743.2 | 99.3% | 98.8% | 230A>G;597_598insGCA;712G>C |
| 3 | human | 23492 | CBX7 | chromobox 7 | NM_001346744.2 | 62.6% | 62.1% | 230A>G;240_241ins279;436G>C |
| 4 | mouse | 52609 | Cbx7 | chromobox 7 | XM_006521144.3 | 89.6% | 88.4% | (many diffs) |
| 5 | mouse | 52609 | Cbx7 | chromobox 7 | XM_011245690.2 | 78.7% | 75% | (many diffs) |
| 6 | mouse | 52609 | Cbx7 | chromobox 7 | XM_011245691.2 | 69.8% | 66.9% | (many diffs) |
| 7 | mouse | 52609 | Cbx7 | chromobox 7 | XM_011245692.2 | 69.8% | 66.9% | (many diffs) |
| 8 | mouse | 52609 | Cbx7 | chromobox 7 | XM_006521147.3 | 68.6% | 68.1% | (many diffs) |
| 9 | mouse | 52609 | Cbx7 | chromobox 7 | NM_144811.3 | 56% | 55.7% | (many diffs) |
| 10 | mouse | 52609 | Cbx7 | chromobox 7 | XM_006521148.3 | 48.7% | 46.6% | (many diffs) |
| 11 | mouse | 52609 | Cbx7 | chromobox 7 | XR_384025.3 | 20.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgtcagcc atcggcgagc aggtgttcgc cgtggagagc atccggaaga 121 agcgcgtgcg gaagggtaaa gtcgagtatc tggtgaagtg gaaaggatgg cccccaaagt 181 acagcacgtg ggagccagaa gagcacatct tggacccccg cctcgtcatg gcctacgagg 241 agaaggagga gagagaccga gcatcggggt ataggaagag aggtccgaaa cccaggcggc 301 ttctgctgca gcggctgtac agcatggacc tgcggagctc ccacaaggcc aagggcaagg 361 agaagctctg cttctccctg acgtgcccac tcggcagcgg gagccctgag ggggtggtca 421 aggcgggggc accTGAGCTG GTGGACAAGG GCCCCTTGGT GCCCACCCTG CCCTTCCCGC 481 TCCGCAAGCC CCGAAAGGCC CACAAGTACC TGCGGCTCTC GCGCAAGAAG TTCCCGCCCC 541 GCGGGCCCAA CCTGGAGAGC CACAGCCATC GACGGGAGCT CTTCCTGCAG GAGCCACCGG 601 CCCCAGACGT CCTGCAGGCG GCTGGCGAGT GGGAGCCTGC TGCGCAGCCC CCTGAAGAGG 661 AGGCAGATGC CGACCTGGCC GAGGGGCCCC CTCCCTGGAC ACCTGCGCTC CCCTCAAGTG 721 AGGTGACCGT GACCGACATC ACCGCCAACT CCATCACCGT CACCTTCCGC GAGGCCCAGC 781 CAGCTGAGGG CTTCTTCCGA GACCGCAGTG GGAAGTTCTG CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAAAACCA AGTACTCCTG GCATAGAGAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt