Construct: ORF TRCN0000465747
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001346.1_s317c1
- Derived from:
- ccsbBroadEn_01913
- DNA Barcode:
- GTATTTCCGCGAATCGGGTCGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C3 (8364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465747
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 100% | 100% | |
| 2 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 83.8% | 100% | (many diffs) |
| 3 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 81.5% | 100% | (many diffs) |
| 4 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 81.5% | 100% | (many diffs) |
| 5 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 81.2% | 100% | (many diffs) |
| 6 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 80.9% | 100% | (many diffs) |
| 7 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 80.9% | 100% | (many diffs) |
| 8 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 80.6% | 100% | (many diffs) |
| 9 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 80.6% | 100% | (many diffs) |
| 10 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 80.2% | 100% | (many diffs) |
| 11 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 82.8% | 100% | (many diffs) |
| 12 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 82.5% | 100% | (many diffs) |
| 13 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 82.5% | 100% | (many diffs) |
| 14 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 82.2% | 100% | (many diffs) |
| 15 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 82.2% | 100% | (many diffs) |
| 16 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 82.2% | 100% | (many diffs) |
| 17 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 81.9% | 100% | (many diffs) |
| 18 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 81.8% | 100% | (many diffs) |
| 19 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 81.8% | 100% | (many diffs) |
| 20 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 80.9% | 100% | (many diffs) |
| 21 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 80.5% | 100% | (many diffs) |
| 22 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 80.2% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGGTCG CGGCAAAGGC GGAAAAGGCT 121 TGGGGAAGGG TGGTGCTAAG CGCCATCGTA AGGTGCTCCG GGATAACATC CAGGGCATTA 181 CAAAACCGGC TATTCGCCGT TTGGCTCGGC GCGGTGGCGT CAAGCGCATT TCCGGTCTTA 241 TCTATGAGGA GACTCGAGGT GTGCTTAAGG TTTTCTTAGA GAACGTTATT CGAGACGCCG 301 TCACCTATAC GGAGCACGCC AAGCGCAAAA CTGTCACAGC CATGGATGTA GTATATGCCC 361 TAAAACGTCA GGGGCGCACT CTGTATGGCT TCGGCGGCTG CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAGTATTT CCGCGAATCG GGTCGGAGAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt