Transcript: Mouse NM_178193.2

Mus musculus histone cluster 1, H4b (Hist1h4b), mRNA.

Source:
NCBI, updated 2017-04-15
Taxon:
Mus musculus (mouse)
Gene:
Hist1h4b (326620)
Length:
450
CDS:
85..396

Additional Resources:

NCBI RefSeq record:
NM_178193.2
NBCI Gene record:
Hist1h4b (326620)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178193.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000376784 TAAGCGCCATCGCAAAGTGCT pLKO_005 132 CDS 100% 2.640 1.584 N Hist1h4b n/a
2 TRCN0000092805 CCATCGCAAAGTGCTGCGCGA pLKO.1 138 CDS 100% 0.000 0.000 N Hist1h4b n/a
3 TRCN0000092804 CCTGGGCAAAGGTGGCGCTAA pLKO.1 114 CDS 100% 0.000 0.000 N Hist1h4b n/a
4 TRCN0000092803 GCCTGGGCAAAGGTGGCGCTA pLKO.1 113 CDS 100% 0.000 0.000 N Hist1h4b n/a
5 TRCN0000376783 CAAAGTGCTGCGCGACAACAT pLKO_005 144 CDS 100% 4.950 2.475 Y Hist1h4b n/a
6 TRCN0000092704 GCTGAAGGTGTTCCTGGAGAA pLKO.1 258 CDS 100% 4.050 2.025 Y Hist1h4h n/a
7 TRCN0000426211 GGTGTTCCTGGAGAACGTGAT pLKO_005 264 CDS 100% 4.050 2.025 Y H4C12 n/a
8 TRCN0000366389 ACAACATCCAGGGCATCACCA pLKO_005 158 CDS 100% 2.640 1.320 Y Hist1h4b n/a
9 TRCN0000106938 GAAGGTGTTCCTGGAGAACGT pLKO.1 261 CDS 100% 2.640 1.320 Y H4C11 n/a
10 TRCN0000092806 GCGACAACATCCAGGGCATCA pLKO.1 155 CDS 100% 1.350 0.675 Y Hist1h4b n/a
11 TRCN0000418041 ATCTCCGGCCTCATCTACGAG pLKO_005 223 CDS 100% 0.880 0.440 Y H4C12 n/a
12 TRCN0000092706 CGGTGTGCTGAAGGTGTTCCT pLKO.1 252 CDS 100% 0.880 0.440 Y Hist1h4h n/a
13 TRCN0000086656 CCGGCCTCATCTACGAGGAGA pLKO.1 227 CDS 100% 0.000 0.000 Y Hist1h4i n/a
14 TRCN0000092707 CGCCGTCACCTACACGGAGCA pLKO.1 291 CDS 100% 0.000 0.000 Y Hist1h4h n/a
15 TRCN0000092787 CTCCGGCCTCATCTACGAGGA pLKO.1 225 CDS 100% 0.000 0.000 Y Hist1h4n n/a
16 TRCN0000086654 GAAGCGCATCTCCGGCCTCAT pLKO.1 216 CDS 100% 0.000 0.000 Y Hist1h4i n/a
17 TRCN0000092784 GCGCATCTCCGGCCTCATCTA pLKO.1 219 CDS 100% 0.000 0.000 Y Hist1h4n n/a
18 TRCN0000092750 GCGCGACAACATCCAGGGCAT pLKO.1 153 CDS 100% 0.000 0.000 Y Hist1h4d n/a
19 TRCN0000096993 GCGACAACATCCAGGGCATTA pLKO.1 155 CDS 100% 10.800 5.400 Y Hist4h4 n/a
20 TRCN0000256283 TGAAGGTGTTCCTGGAGAATG pLKO_005 260 CDS 100% 10.800 5.400 Y H4C15 n/a
21 TRCN0000181157 CTGAAGGTGTTCCTGGAGAAT pLKO.1 259 CDS 100% 4.950 2.475 Y H4C15 n/a
22 TRCN0000096990 CGCGACAACATCCAGGGCATT pLKO.1 154 CDS 100% 1.350 0.675 Y Hist4h4 n/a
23 TRCN0000092752 CTCATCTACGAGGAGACCCGT pLKO.1 232 CDS 100% 0.220 0.110 Y Hist1h4d n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178193.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01912 pDONR223 100% 90.6% 100% None (many diffs) n/a
2 ccsbBroad304_01912 pLX_304 0% 90.6% 100% V5 (many diffs) n/a
3 TRCN0000472437 ACTCCACAACGATCTTTCCTTCAT pLX_317 100% 90.6% 100% V5 (many diffs) n/a
4 ccsbBroadEn_07216 pDONR223 100% 89.9% 100% None (many diffs) n/a
5 ccsbBroad304_07216 pLX_304 0% 89.9% 100% V5 (many diffs) n/a
6 TRCN0000468808 CGAAACCTGTGAAAGAAATAGCCA pLX_317 100% 89.9% 100% V5 (many diffs) n/a
7 ccsbBroadEn_01883 pDONR223 100% 89.6% 100% None (many diffs) n/a
8 ccsbBroad304_01883 pLX_304 0% 89.6% 100% V5 (many diffs) n/a
9 TRCN0000470786 AAACACGGTAGAGCCAAGTCGACG pLX_317 100% 89.6% 100% V5 (many diffs) n/a
10 ccsbBroadEn_01916 pDONR223 100% 86.7% 100% None (many diffs) n/a
11 ccsbBroad304_01916 pLX_304 0% 86.7% 100% V5 (many diffs) n/a
12 TRCN0000468855 AAAAGGGCACCCCGTTGTGATCCC pLX_317 100% 86.7% 100% V5 (many diffs) n/a
13 ccsbBroadEn_05703 pDONR223 100% 86.4% 100% None (many diffs) n/a
14 ccsbBroad304_05703 pLX_304 0% 86.4% 100% V5 (many diffs) n/a
15 TRCN0000478553 TTTATTGTGCGATTTATCCGATTA pLX_317 82.9% 86.4% 100% V5 (many diffs) n/a
16 ccsbBroadEn_01911 pDONR223 100% 85.7% 100% None (many diffs) n/a
17 ccsbBroad304_01911 pLX_304 0% 85.7% 100% V5 (many diffs) n/a
18 TRCN0000472587 AGGCTCCAGTCGACTTCTCCCACC pLX_317 100% 85.7% 100% V5 (many diffs) n/a
19 ccsbBroadEn_01910 pDONR223 100% 85.7% 100% None (many diffs) n/a
20 ccsbBroad304_01910 pLX_304 0% 85.7% 100% V5 (many diffs) n/a
21 TRCN0000470331 GGAGTACAACATTTCGGGTCCGAA pLX_317 100% 85.7% 100% V5 (many diffs) n/a
22 ccsbBroadEn_11268 pDONR223 100% 83.8% 100% None (many diffs) n/a
23 ccsbBroad304_11268 pLX_304 0% 83.8% 100% V5 (many diffs) n/a
24 TRCN0000473699 AAATTGTTGTTGTCACATGCCGTA pLX_317 100% 83.8% 100% V5 (many diffs) n/a
25 ccsbBroadEn_01915 pDONR223 100% 83.8% 100% None (many diffs) n/a
26 ccsbBroad304_01915 pLX_304 0% 83.8% 100% V5 (many diffs) n/a
27 TRCN0000475279 TATAGCGGGACGTGTAGAGTAGCT pLX_317 27.6% 83.8% 100% V5 (many diffs) n/a
28 ccsbBroadEn_01914 pDONR223 100% 82.8% 100% None (many diffs) n/a
29 ccsbBroad304_01914 pLX_304 0% 82.8% 100% V5 (many diffs) n/a
30 TRCN0000470478 ACCAGTGCAGTTCTATCGAGTATT pLX_317 100% 82.8% 100% V5 (many diffs) n/a
31 ccsbBroadEn_01913 pDONR223 100% 82.2% 100% None (many diffs) n/a
32 ccsbBroad304_01913 pLX_304 0% 82.2% 100% V5 (many diffs) n/a
33 TRCN0000465747 GTATTTCCGCGAATCGGGTCGGAG pLX_317 100% 82.2% 100% V5 (many diffs) n/a
34 ccsbBroadEn_07227 pDONR223 100% 82% 99% None (many diffs) n/a
35 ccsbBroad304_07227 pLX_304 0% 82% 99% V5 (many diffs) n/a
36 TRCN0000466667 ACAGCTATGCTGGGTCCGATAGTT pLX_317 69.3% 82% 99% V5 (many diffs) n/a
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