Construct: ORF TRCN0000465759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015731.1_s317c1
- Derived from:
- ccsbBroadEn_04982
- DNA Barcode:
- CGCCTGCGCACATCACTACTGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD19 (146212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146212 | KCTD19 | potassium channel tetrameri... | NM_001100915.3 | 100% | 100% | |
| 2 | mouse | 279499 | Kctd19 | potassium channel tetrameri... | NM_001301173.1 | 86.5% | 88.7% | (many diffs) |
| 3 | mouse | 279499 | Kctd19 | potassium channel tetrameri... | XM_011248400.2 | 85.4% | 87.5% | (many diffs) |
| 4 | mouse | 279499 | Kctd19 | potassium channel tetrameri... | NM_177791.3 | 84.4% | 86.6% | (many diffs) |
| 5 | mouse | 279499 | Kctd19 | potassium channel tetrameri... | XM_011248399.2 | 83.4% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2847
- ORF length:
- 2778
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggagtct ggcatggctc atgaatcagc agaggacttg tttcatttca 121 acgtaggggg ctggcatttc tcagttccca gaagcaaact ctctcagttt ccagactccc 181 tgctgtggaa agaggcttca gccttgacct cttcagaaag ccagaggcta tttatcgaca 241 gagatggttc cacatttagg cacgtgcact attacctcta cacctccaaa ctctccttct 301 ccagttgtgc agaactgaac ttgctgtatg agcaagcatt gggtttgcag ctgatgcctt 361 tgctgcagac tctagataac ctgaaggaag ggaaacacca tctacgcgta cggcctgcag 421 acctacctgt tgctgagaga gcatctctga actactggcg tacatggaag tgtattagca 481 aaccctcaga atttccaatt aaaagcccag cctttacagg cctacatgat aaggcacctc 541 tggggctcat ggacacaccc ctgttagaca cagaagagga ggtgcactac tgcttcctgc 601 ccctagacct ggtggccaaa tatcccagcc tagtgactga agacaacctg ctgtggctgg 661 ctgagacggt ggccctcatc gagtgcgagt gcagcgagtt ccgcttcatt gtgaattttc 721 ttcgctcaca gaagatttta ctaccggata atttctccaa cattgatgta ttagaagcag 781 aagtggaaat tctggaaatc cctgcactca ctgaagccgt aaggtggtac cggatgaaca 841 tgggtggctg ttccccgacc acctgttctc ccctgagccc cgggaagggg gcccgcacag 901 ccagcctgga gtccgtgaaa ccgctctaca caatggccct gggtctgctg gtcaagtacc 961 cggactctgc gctgggccag cttcgcatcg agagcacgct agacggaagc cgactgtaca 1021 tcacagggaa tggcgtcctc tttcagcacg tcaagaactg gctggggact tgccggctgc 1081 ccctgacaga gaccatttcc gaggtatatg agctctgtgc cttcctagac aaaagggaca 1141 tcacctacga gccaatcaaa gttgctttga agactcatct ggagccaagg actttggcac 1201 ccatggatgt gctcaatgag tggacggcag agatcactgt gtattcccca caacagatca 1261 tcaaagtgta tgttggaagc cactggtacg caaccaccct gcagacactg ctgaagtatc 1321 cagaactgct gtccaaccct cagagagtgt actggatcac atatggacaa accctgctca 1381 tccacgggga tggccagatg ttccgacaca ttctcaactt cctgagactt ggcaaactgt 1441 ttttaccatc tgaatttaag gaatggcccc tcttctgcca ggaggtggag gaataccaca 1501 ttccatccct ctcagaagcc cttgcacaat gtgaagcata caagtcatgg actcaggaga 1561 aagaatctga aaatgaagaa gctttttcca tcaggaggct gcatgtggtg acagaagggc 1621 cagggtcact ggtggagttc agtagagaca ctaaagaaac cacagcctac atgcctgtgg 1681 acttcgaaga ctgcagtgac aggactccat ggaacaaggc taagggaaac ctggtcaggt 1741 ccaaccagat ggatgaggct gagcagtaca ctcggcccat ccaggtgtcc ctatgccgaa 1801 atgccaagag ggctggcaac cctagcacat actcacactg ccgtggcttg tgtaccaatc 1861 ctggacactg ggggagccac cctgagagcc ccccaaagaa gaaatgcacc acaatcaacc 1921 tcacacagaa atctgaaacc aaagaccctc ccgccactcc catgcaaaaa ctcatctccc 1981 tggtgagaga atgggacatg gtcaattgca aacagtggga attccagcca ctgacagcca 2041 cacggagcag ccccttggag gaggccaccc tgcagctccc cttgggaagc gaggctgctt 2101 cccagcccag cacctcagct gcctggaaag cccattccac agcctcagag aaggatccag 2161 gaccacaggc aggggctgga gctggagcga aagacaaggg gccagagcca accttcaagc 2221 catacttacc cccaaaaaga gctggcaccc tgaaggactg gagcaagcag aggaccaagg 2281 agagagaaag ccctgcccct gagcagcctc tgcccgaggc cagtgaggtg gacagcctag 2341 gggttatcct caaagtgact cacccccccg tggtgggcag cgatggcttc tgcatgttct 2401 ttgaggacag catcatctat accacggaga tggacaacct caggcacaca acacccacag 2461 ccagtcccca gccccaagaa gtgactttcc TGAGTTTCTC TCTGTCCTGG GAAGAGATGT 2521 TTTATGCACA GAAATGTCAC TGCTTCCTGG CTGACATCAT CATGGATTCC ATCAGGCAAA 2581 AGGACCCCAA AGCCATCACA GCCAAGGTGG TCTCCCTGGC CAATCGGCTG TGGACCCTGC 2641 ACATCAGCCC CAAGCAGTTT GTGGTAGATT TGCTGGCCAT CACCGGCTTC AAGGATGACC 2701 GGCACACCCA GGAGCGCCTG TACAGCTGGG TGGAGCTTAC ACTGCCCTTC GCCAGGAAAT 2761 ATGGCCGATG CATGGACCTG CTCATCCAGA GGGGCCTGTC TAGGTCTGTC TCTTACTCCA 2821 TCCTGGGAAA GTACCTACAA GAGGACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2881 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2941 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCCTGCG 3001 CACATCACTA CTGGCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3061 aagatt