Construct: ORF TRCN0000465792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010446.1_s317c1
- Derived from:
- ccsbBroadEn_04793
- DNA Barcode:
- GTGGAGCCAGTCCTACCCGTGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYB5D2 (124936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | NM_144611.4 | 100% | 100% | |
| 2 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | XM_006721447.3 | 77% | 69.7% | (many diffs) |
| 3 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | NM_001254755.2 | 57.5% | 57.5% | 0_1ins336 |
| 4 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | NM_001254756.1 | 57.5% | 57.5% | 0_1ins336 |
| 5 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | XM_011523649.2 | 57.5% | 57.5% | 0_1ins336 |
| 6 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | XM_017024180.1 | 57.5% | 57.5% | 0_1ins336 |
| 7 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | XM_017024182.2 | 57.5% | 57.5% | 0_1ins336 |
| 8 | human | 124936 | CYB5D2 | cytochrome b5 domain contai... | XM_017024183.2 | 51.1% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gaggtgcgga ggccgtgggc ttttgttggg cctggctgta gccgcagcag 121 cggtaatggc agcacggctt atgggctggt ggggtccccg cgctggcttt cgccttttca 181 taccggagga gctgtctcgc taccgcggcg gcccagggga cccgggcctg tacttggcgt 241 tgctcggccg tgtctacgat gtgtcctccg gccggaggca ctacgagcct gggtcccact 301 atagcggctt cgcaggccga gacgcatcca gagctttcgt gaccggggac tgttctgaag 361 caggcctcgt ggatgacgta tccgacctgt cagccgctga gatgctgaca cttcacaatt 421 ggctttcatt ctatgagaag aattatgtgt gtgttgggag ggtgacagga cggttcTACG 481 GAGAGGATGG GCTGCCCACC CCGGCACTGA CCCAGGTAGA AGCTGCGATC ACCAGAGGCT 541 TGGAGGCCAA CAAACTACAG CTGCAAGAGA AGCAGACATT CCCGCCGTGC AACGCGGAGT 601 GGAGCTCAGC CAGGGGCAGC CGGCTCTGGT GCTCCCAGAA GAGTGGAGGT GTGAGCAGAG 661 ACTGGATTGG CGTCCCCAGG AAGCTGTATA AGCCAGGTGC TAAGGAGCCC CGCTGCGTGT 721 GTGTGAGAAC CACCGGCCCC CCTAGTGGCC AGATGCCGGA CAACCCTCCA CACAGAAATC 781 GTGGGGACCT GGACCACCCA AACTTGGCAG AGTACACAGG CTGCCCACCG CTAGCCATCA 841 CATGCTCCTT TCCACTCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTGGAGC CAGTCCTACC 1021 CGTGGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt