Construct: ORF TRCN0000465820
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009822.1_s317c1
- Derived from:
- ccsbBroadEn_08761
- DNA Barcode:
- TTCTAATCCAAGTCATCCATAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM160B1 (57700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465820
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57700 | FAM160B1 | family with sequence simila... | NM_001135051.2 | 99.9% | 99.8% | 1222C>A |
| 2 | human | 57700 | FAM160B1 | family with sequence simila... | NM_020940.4 | 95.9% | 94.4% | (many diffs) |
| 3 | mouse | 226252 | Fam160b1 | family with sequence simila... | NM_145505.4 | 80.5% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2280
- ORF length:
- 2214
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctccaagttc acctccatcc tgcagcacgc cgtggaggcg cttgcacctt 121 ctcttccttt acaagaagat tttgtttatc actggaaggc aattacccat tactacatag 181 agacttcaga tgataaagcc ccagtgaccg atacaaatat tccatcgcat ctggaacaga 241 tgttagatat actggttcaa gaagaaaatg aacgggaatc tggagagaca gggccatgta 301 tggaatattt gcttcatcac aagatcttgg aaacattata taccttgggg aaagctgatt 361 gtcctccggg aatgaaacag caggttttgg ttttctatac gaaacttctg ggaagaatcc 421 ggcagccact acttccacac attaacgtgc acaggccagt gcagaaatta attagactct 481 gtggtgaagt cctagcaaca ccaacagaaa atgaagagat tcagtttctt tgcattgtgt 541 gtgcgaagct gaaacaggac ccctacctgg ttaacttttt cctagagaat aagatgaaat 601 cattggcttc caaaggagta ccaaatgtaa tttcagaaga tacattaaaa ggtcaggatt 661 ccttgtcaac agatacagga cagtcccgtc aaccagagga actatctggt gctactggaa 721 tggagcaaac agaattggaa gatgagcctc ctcatcagat ggatcacctg tccacaagct 781 tggataacct cagtgtcacc tcactgccag aggcctcggt tgtttgtcca aatcaggatt 841 acaatttagt gaattctttg ttaaatctta ctagaagtcc tgatggcaga atagctgtga 901 aggcatgtga aggcttgatg ctgttagtaa gtttgccaga gcctgcggct gcaaagtgcc 961 ttacacagag cacttgcttg tgtgaactac tgacagacag acttgcctcc ctgtacaagg 1021 ccctacctca gtcagtggat ccgttagata ttgaaaccgt ggaagcaatt aactggggct 1081 tggactcata tagtcataaa gaagatgctt cagcatttcc aggaaaacga gccttaattt 1141 catttctttc ctggtttgat tattgtgatc agctcattaa ggaagcccaa aagactgctg 1201 ctgttgctct tgccaaagct gttcatgaaa gatttttcat tggtgttatg gaacctcaat 1261 taatgcaaac ttctgagatg ggtattatca catccactgc tctgcttcat cgcatcgttc 1321 ggcaagtgac ctctgatgtt ttgcttcaag aaatggtgtt ttttatcctt ggagaacaga 1381 gggaaccaga aactctggca gaaatcagca gacatccttt aaggcatagg ttaattgaac 1441 attgtgatca catatctgat gagataagca taatgacatt acgaatgttt gaacatcttt 1501 tacaaaaacc caatgagcac attctttaca acttggtctt gagaaatctt gaagaaagaa 1561 attatacaga atataaacct ttgtgcccag aagataaaga tgtggtagaa aatggattga 1621 tagcaggagc agtagatctg gaagaagatc cattatttac tgacatttca ccagaaaaca 1681 ctttgccaaa ccaagagtgg cttagttctt cacctcctgc tactccagac caccccaaaa 1741 atgatggaaa aactgaagtt cataaaattg taaatagttt tctctgtctg gtaccggatg 1801 acgcaaaatc ctcctaccat gttgagggca caggatatga cacttaccTC CGAGACGCTC 1861 ATAGGCAGTT CCGAGACTAC TGTGCTATCT GCTTAAGATG GGAGTGGCCT GGGTCTCCAA 1921 AAGCATTGGA AAAGTGCAAT TTAGAAGCTG CTTTCTTTGA AGGTCATTTT TTGAAAGTGC 1981 TGTTCGACAG AATGGGAAGA ATTCTTGATC AGCCATATGA TGTAAACTTA CAAGTAACCT 2041 CAGTGTTATC TAGACTTTCT CTCTTCCCTC ATCCACACAT CCACGAGTAC CTTTTGGATC 2101 CTTACGTGAA CCTCGCTCCT GGCTGTAGAT CTCTCTTCTC TGTAATTGTC AGGGTTGTTG 2161 GAGACCTTAT GCTTCGAATC CAGCGTATTC AAGACTTTAC TCCCAAGCTT CTGTTAGTCA 2221 GAAAGCGGTT ACTTGGTTTG GAACCTGAAG GCCCTATGCA GACGTTGCCT TCATGGTGGT 2281 ATCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2341 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2401 TTTATATATC TTGTGGAAAG GACGATTCTA ATCCAAGTCA TCCATAGCTA CGCGTTAAGT 2461 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt