Transcript: Human NM_020940.4

Homo sapiens family with sequence similarity 160 member B1 (FAM160B1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
FAM160B1 (57700)
Length:
5774
CDS:
300..2597

Additional Resources:

NCBI RefSeq record:
NM_020940.4
NBCI Gene record:
FAM160B1 (57700)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020940.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365543 CCTTTAAGGCATAGGTTAATT pLKO_005 1650 CDS 100% 15.000 21.000 N FAM160B1 n/a
2 TRCN0000365612 GGAACCTGAAGGCCCTATTAT pLKO_005 2474 CDS 100% 15.000 21.000 N FAM160B1 n/a
3 TRCN0000129856 GCCATATAGTACTCACCTTAT pLKO.1 5047 3UTR 100% 10.800 15.120 N FAM160B1 n/a
4 TRCN0000129553 CGGGAATGAAACAGCAGGTTT pLKO.1 601 CDS 100% 4.950 6.930 N FAM160B1 n/a
5 TRCN0000130612 CTGGAAGGCAATTACCCATTA pLKO.1 386 CDS 100% 10.800 8.640 N FAM160B1 n/a
6 TRCN0000131161 GCCTCGGTTGTTTGTCCAAAT pLKO.1 1047 CDS 100% 10.800 8.640 N FAM160B1 n/a
7 TRCN0000370705 GTCATGACTTTGATGATATTT pLKO_005 2905 3UTR 100% 15.000 10.500 N FAM160B1 n/a
8 TRCN0000370759 TCTGGAAGAAGATCCATTATT pLKO_005 1871 CDS 100% 15.000 10.500 N FAM160B1 n/a
9 TRCN0000370704 TGCTTCCTCCACACCATAAAT pLKO_005 2579 CDS 100% 15.000 10.500 N FAM160B1 n/a
10 TRCN0000365611 TTGGACTCATATAGTCATAAA pLKO_005 1314 CDS 100% 13.200 9.240 N FAM160B1 n/a
11 TRCN0000130062 GCACCGTCATAACAATTGATT pLKO.1 4867 3UTR 100% 5.625 3.938 N FAM160B1 n/a
12 TRCN0000129574 CCGCAGCACTTTCTTCTTCAT pLKO.1 3853 3UTR 100% 4.950 3.465 N FAM160B1 n/a
13 TRCN0000128331 GCATGTTTGCATATACGCATT pLKO.1 3967 3UTR 100% 4.050 2.835 N FAM160B1 n/a
14 TRCN0000131059 CAGAGGAACTATCTGGTGCTA pLKO.1 928 CDS 100% 2.640 1.848 N FAM160B1 n/a
15 TRCN0000129385 GAACCAGAAACTCTGGCAGAA pLKO.1 1617 CDS 100% 4.050 2.430 N FAM160B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020940.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08761 pDONR223 100% 95.9% 94.4% None (many diffs) n/a
2 ccsbBroad304_08761 pLX_304 0% 95.9% 94.4% V5 (many diffs) n/a
3 TRCN0000465820 TTCTAATCCAAGTCATCCATAGCT pLX_317 19.7% 95.9% 94.4% V5 (many diffs) n/a
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