Construct: ORF TRCN0000465866

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001885.1_s317c1
Derived from:
ccsbBroadEn_04665
DNA Barcode:
CCAGCAGTGCTCGCAGGGGAAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
OSBPL8 (114882)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465866
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 114882 OSBPL8 oxysterol binding protein l... NM_020841.5 100% 100%
2 human 114882 OSBPL8 oxysterol binding protein l... XM_005268621.5 100% 100%
3 human 114882 OSBPL8 oxysterol binding protein l... NM_001319653.2 99.6% 99.6% 79_80insCTGTTGTAG
4 human 114882 OSBPL8 oxysterol binding protein l... XM_017018769.2 99.6% 99.6% 79_80insCTGTTGTAG
5 human 114882 OSBPL8 oxysterol binding protein l... XM_011537856.3 98.5% 98.5% 43_81del
6 human 114882 OSBPL8 oxysterol binding protein l... XM_011537857.3 98.5% 98.5% 43_81del
7 human 114882 OSBPL8 oxysterol binding protein l... XM_011537855.3 98.2% 98.2% 43_81del;118_119insCTGTTGTAG
8 human 114882 OSBPL8 oxysterol binding protein l... XM_017018768.2 97.4% 97.4% 1_69del
9 human 114882 OSBPL8 oxysterol binding protein l... NM_001003712.2 95.2% 95.2% 0_1ins126
10 human 114882 OSBPL8 oxysterol binding protein l... NM_001319652.2 95.2% 95.2% 0_1ins126
11 human 114882 OSBPL8 oxysterol binding protein l... XM_006719224.3 95.2% 95.2% 0_1ins126
12 human 114882 OSBPL8 oxysterol binding protein l... XM_024448829.1 95.2% 95.2% 0_1ins126
13 human 114882 OSBPL8 oxysterol binding protein l... NM_001319655.2 94.6% 90.8% (many diffs)
14 mouse 237542 Osbpl8 oxysterol binding protein-l... NM_175489.3 89.3% 96% (many diffs)
15 mouse 237542 Osbpl8 oxysterol binding protein-l... XM_006513637.2 89% 95.7% (many diffs)
16 mouse 237542 Osbpl8 oxysterol binding protein-l... XM_006513638.2 85.1% 88.9% (many diffs)
17 mouse 237542 Osbpl8 oxysterol binding protein-l... NM_001003717.1 85.1% 91.9% (many diffs)
18 mouse 237542 Osbpl8 oxysterol binding protein-l... XM_006513639.2 85.1% 91.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2736
ORF length:
2667
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagggaggt ttggcagatg gagaacctga tcgaacttcg cttcttggtg
121atagcaaaga tgtccttggg ccatcaactg ttgtagcaaa cagtgacgaa tctcagcttc
181tgacaccagg aaagatgagt cagcgccaag gaaaagaagc ttatccaacg ccaaccaaag
241atttgcatca gccatctctt agtccagcaa gtcctcatag ccagggtttt gaaagaggga
301aggaagatat ttctcaaaat aaagatgaat cttcactttc tatgtcaaag agcaagtctg
361aatctaaact ttataatggc tcagagaagg acagttcaac ttcaagcaaa ctcacaaaaa
421aagaatctct taaggtacaa aagaaaaatt accgagaaga aaagaaaaga gccacaaagg
481agctgctcag tacaatcaca gatccttctg ttattgttat ggctgattgg ttaaagattc
541gtggtactct aaagagctgg accaagttat ggtgtgtgtt gaaacctggg gtgctactga
601tctataaaac ccaaaaaaat ggtcagtggg taggaacagt tcttctgaat gcctgtgaaa
661tcattgaacg tccatcaaaa aaggatggct tttgtttcaa acttttccat cctttggagc
721aatctatttg ggcagtgaag ggtccaaaag gtgaagcggt tggatccatt actcaaccct
781tacctagcag ttatttgatc atccgagcta cttcagagtc agatggaagg tgctggatgg
841atgctttgga gttggctttg aaatgttcta gtcttcttaa acgtacaatg atcagagaag
901gaaaggaaca tgacctgagc gtttcatcag atagcacaca tgtgactttc tatggcttac
961tacgtgctaa caatctccac agtggtgata acttccagtt aaatgatagt gaaattgaac
1021gacaacattt taaggaccaa gatatgtatt ctgataaatc tgataaagaa aatgatcaag
1081aacatgatga gtctgataat gaggtgatgg ggaaaagtga agaaagtgac acagatacat
1141cagaaagaca agatgactca tatatcgaac ctgagcctgt tgagccttta aaggagacta
1201cctacactga acagagccat gaagaacttg gagaggcagg tgaggcttct caaacagaaa
1261ctgtatctga agaaaacaaa agccttatct ggacactatt gaaacaagtc cgtcctggca
1321tggacctatc caaggtggtt ctgcctacat ttattttgga accccgttct ttcctggata
1381aactttcaga ttactactat catgcagatt tcctatctga ggcagctctt gaagaaaatc
1441cttatttccg tttgaagaaa gtagtgaaat ggtatttgtc aggattctat aaaaagccaa
1501agggactgaa gaaaccttat aatcctatac ttggcgagac tttccgttgt ttatggattc
1561atcccagaac aaacagcaaa actttttata ttgctgaaca ggtgtcccat catccaccaa
1621tatctgcctt ttatgttagt aatcgaaaag atggattttg ccttagcggt agtatcctgg
1681ctaagtctaa gttttatgga aactcattat ctgcaatatt agagggagaa gcacggttaa
1741ctttcttgaa tagaggtgaa gattatgtaa tgacaatgcc atacgctcat tgtaaaggaa
1801ttctttatgg tacaatgaca ctggagcttg gtggaacagt caatattaca tgtcaaaaaa
1861ctggatacag tgcaatactt gaatttaaac taaagccatt cctagggagt agtgactgtg
1921ttaatcaaat atcagggaaa cttaaactgg gaaaagaagt cctagctact ttggaaggtc
1981attgggatag tgaagttttt attactgata aaaagactga taattcagag gttttctgga
2041atccaacacc tgacattaag caatggagat taataaggca cactgtaaaa tttgaagaac
2101agggagattt tgaatcagag aaactctggc aacgggtaac tcgagccata aatgccaaag
2161accaaactga agctacccaa gagaagtatg ttttggaaga agctcaaaga caagctgcca
2221gggatcggaa aacaaaaaat gaagagtggt cttgcaaatt atttgaactt gatccactca
2281caggagaatg gcattacaag tttgcagata cccgaccatg ggacccactt aatgatatga
2341tacagtttGA AAAAGATGGT GTTATTCAGA CCAAAGTGAA ACATCGTACT CCAATGGTTA
2401GCGTCCCCAA AATGAAACAT AAGCCAACCA GGCAACAGAA GAAAGTAGCA AAAGGCTATT
2461CCTCCCCAGA ACCTGACATT CAAGACTCCT CTGGAAGTGA AGCTCAATCA GTAAAACCAA
2521GTACAAGAAG AAAGAAAGGA ATAGAACTGG GAGACATTCA GAGTTCCATC GAATCTATAA
2581AACAAACACA GGAAGAAATT AAAAGAAATA TTATGGCTCT TCGAAATCAT TTAGTTTCAA
2641GCACACCGGC CACGGATTAT TTTCTGCAAC AAAAAGACTA CTTCATCATT TTCCTCCTGA
2701TTTTGCTTCA AGTCATAATA AACTTCATGT TCAAGTTGCC AACTTTCTTG TACAAAGTGG
2761TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2821CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2881ACCAGCAGTG CTCGCAGGGG AAGCCACGCG TTAAGTCgac aatcaacctc tggattacaa
2941aatttgtgaa agatt

Download FASTA (ORF) (Full)