Construct: ORF TRCN0000465868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017483.1_s317c1
- Derived from:
- ccsbBroadEn_06159
- DNA Barcode:
- TCGACCCCGAATAAACTTGGCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTTN (2017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465868
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2017 | CTTN | cortactin | NM_138565.2 | 99.8% | 100% | 786A>G;1347C>T |
| 2 | human | 2017 | CTTN | cortactin | NM_005231.4 | 93.1% | 93.2% | 781_891del;898C>A;1458C>T |
| 3 | human | 2017 | CTTN | cortactin | XM_006718447.3 | 92.7% | 92.7% | 669_670ins111;1236C>T |
| 4 | human | 2017 | CTTN | cortactin | NM_001184740.2 | 80% | 79% | (many diffs) |
| 5 | human | 2017 | CTTN | cortactin | XM_006718448.4 | 58.4% | 56.6% | (many diffs) |
| 6 | human | 2017 | CTTN | cortactin | XM_017017312.2 | 52.9% | 50.9% | (many diffs) |
| 7 | mouse | 13043 | Cttn | cortactin | NM_001252572.1 | 85% | 92% | (many diffs) |
| 8 | mouse | 13043 | Cttn | cortactin | NM_007803.5 | 79.4% | 85.8% | (many diffs) |
| 9 | mouse | 13043 | Cttn | cortactin | XM_006508473.2 | 78.6% | 84.7% | (many diffs) |
| 10 | mouse | 13043 | Cttn | cortactin | XM_006508474.2 | 52.2% | 55.1% | (many diffs) |
| 11 | mouse | 13043 | Cttn | cortactin | XM_006508475.2 | 48.4% | 53.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1605
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gaaagcttca gcaggccacg ctgtgtccat cgcccaggat gacgcggggg 121 ccgatgactg ggagaccgac cctgattttg tgaatgatgt gagtgagaag gagcaaagat 181 ggggtgccaa gacggtgcag ggctccgggc accaggagca tatcaacata cacaagctga 241 gggagaatgt ctttcaagag catcagaccc ttaaggagaa ggaacttgaa acaggaccaa 301 aagcttccca tggctatgga gggaaatttg gtgtggaaca agaccgaatg gataagtcag 361 ctgtcggcca cgaatatcag tcgaaacttt ccaagcactg ctcgcaggtg gactcggtcc 421 gtggcttcgg aggcaagttt ggtgtccaga tggacagagt tgatcagtct gctgtaggct 481 ttgaatacca ggggaagact gagaagcatg cctcccagaa agactactcc agtggttttg 541 gcggcaagta tggcgtgcag gccgaccgag tagacaagag cgcggtgggc ttcgactacc 601 agggcaagac ggagaagcac gagtcacaga gagattactc caaaggtttc ggcggcaaat 661 acggtatcga caaggacaaa gtggataaga gcgccgttgg ctttgagtat caaggcaaaa 721 cggagaagca cgagtcccag aaagactatg tgaaagggtt tggaggaaaa tttggtgtgc 781 agacagacag acaagacaaa tgtgcccttg gctgggatca ccaggagaaa ttgcagctgc 841 atgaatccca gaaagactac tccaaaggat tcggcgggaa gtatggggtg cagaaggatc 901 ggatggataa gaatgcgtca acctttgagg atgtcaccca ggtgtcctct gcctaccaga 961 agacagtacc tgtcgaagct gtgaccagca aaacaagtaa catcagagct aactttgaaa 1021 acctcgctaa ggagaaagag caggaggaca ggcggaaggc ggaggcggag agagcccagc 1081 ggatggccaa ggagcggcag gagcaggaag aggccaggag gaagctggag gagcaagcca 1141 gagccaaaac gcaaacgccc cctgtgtcgc ccgcacctca gccaaccgag gagaggctgc 1201 cctcgagccC CGTCTATGAG GATGCGGCTT CCTTCAAGGC AGAGCTGAGC TACAGAGGCC 1261 CTGTGAGTGG GACGGAGCCG GAGCCCGTGT ACAGCATGGA GGCCGCTGAC TACCGAGAGG 1321 CCAGCAGCCA GCAGGGCCTG GCCTATGCCA CAGAGGCTGT CTATGAAAGC GCAGAGGCCC 1381 CGGGCCACTA TCCCGCAGAG GACAGCACCT ATGATGAGTA CGAGAACGAT CTGGGGATCA 1441 CAGCCGTCGC CCTGTACGAC TACCAGGCTG CGGGCGATGA TGAGATCTCA TTTGACCCTG 1501 ATGACATCAT CACCAACATC GAGATGATTG ACGACGGCTG GTGGCGCGGG GTGTGCAAGG 1561 GCCGGTACGG GCTCTTCCCA GCCAACTATG TGGAGCTGCG GCAGTACCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA TCGACCCCGA ATAAACTTGG CATCACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt