Construct: ORF TRCN0000465868

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017483.1_s317c1
Derived from:
ccsbBroadEn_06159
DNA Barcode:
TCGACCCCGAATAAACTTGGCATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CTTN (2017)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465868
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2017 CTTN cortactin NM_138565.2 99.8% 100% 786A>G;1347C>T
2 human 2017 CTTN cortactin NM_005231.4 93.1% 93.2% 781_891del;898C>A;1458C>T
3 human 2017 CTTN cortactin XM_006718447.3 92.7% 92.7% 669_670ins111;1236C>T
4 human 2017 CTTN cortactin NM_001184740.2 80% 79% (many diffs)
5 human 2017 CTTN cortactin XM_006718448.4 58.4% 56.6% (many diffs)
6 human 2017 CTTN cortactin XM_017017312.2 52.9% 50.9% (many diffs)
7 mouse 13043 Cttn cortactin NM_001252572.1 85% 92% (many diffs)
8 mouse 13043 Cttn cortactin NM_007803.5 79.4% 85.8% (many diffs)
9 mouse 13043 Cttn cortactin XM_006508473.2 78.6% 84.7% (many diffs)
10 mouse 13043 Cttn cortactin XM_006508474.2 52.2% 55.1% (many diffs)
11 mouse 13043 Cttn cortactin XM_006508475.2 48.4% 53.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1605
ORF length:
1539
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg gaaagcttca gcaggccacg ctgtgtccat cgcccaggat gacgcggggg
121ccgatgactg ggagaccgac cctgattttg tgaatgatgt gagtgagaag gagcaaagat
181ggggtgccaa gacggtgcag ggctccgggc accaggagca tatcaacata cacaagctga
241gggagaatgt ctttcaagag catcagaccc ttaaggagaa ggaacttgaa acaggaccaa
301aagcttccca tggctatgga gggaaatttg gtgtggaaca agaccgaatg gataagtcag
361ctgtcggcca cgaatatcag tcgaaacttt ccaagcactg ctcgcaggtg gactcggtcc
421gtggcttcgg aggcaagttt ggtgtccaga tggacagagt tgatcagtct gctgtaggct
481ttgaatacca ggggaagact gagaagcatg cctcccagaa agactactcc agtggttttg
541gcggcaagta tggcgtgcag gccgaccgag tagacaagag cgcggtgggc ttcgactacc
601agggcaagac ggagaagcac gagtcacaga gagattactc caaaggtttc ggcggcaaat
661acggtatcga caaggacaaa gtggataaga gcgccgttgg ctttgagtat caaggcaaaa
721cggagaagca cgagtcccag aaagactatg tgaaagggtt tggaggaaaa tttggtgtgc
781agacagacag acaagacaaa tgtgcccttg gctgggatca ccaggagaaa ttgcagctgc
841atgaatccca gaaagactac tccaaaggat tcggcgggaa gtatggggtg cagaaggatc
901ggatggataa gaatgcgtca acctttgagg atgtcaccca ggtgtcctct gcctaccaga
961agacagtacc tgtcgaagct gtgaccagca aaacaagtaa catcagagct aactttgaaa
1021acctcgctaa ggagaaagag caggaggaca ggcggaaggc ggaggcggag agagcccagc
1081ggatggccaa ggagcggcag gagcaggaag aggccaggag gaagctggag gagcaagcca
1141gagccaaaac gcaaacgccc cctgtgtcgc ccgcacctca gccaaccgag gagaggctgc
1201cctcgagccC CGTCTATGAG GATGCGGCTT CCTTCAAGGC AGAGCTGAGC TACAGAGGCC
1261CTGTGAGTGG GACGGAGCCG GAGCCCGTGT ACAGCATGGA GGCCGCTGAC TACCGAGAGG
1321CCAGCAGCCA GCAGGGCCTG GCCTATGCCA CAGAGGCTGT CTATGAAAGC GCAGAGGCCC
1381CGGGCCACTA TCCCGCAGAG GACAGCACCT ATGATGAGTA CGAGAACGAT CTGGGGATCA
1441CAGCCGTCGC CCTGTACGAC TACCAGGCTG CGGGCGATGA TGAGATCTCA TTTGACCCTG
1501ATGACATCAT CACCAACATC GAGATGATTG ACGACGGCTG GTGGCGCGGG GTGTGCAAGG
1561GCCGGTACGG GCTCTTCCCA GCCAACTATG TGGAGCTGCG GCAGTACCCA ACTTTCTTGT
1621ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1681AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1741GAAAGGACGA TCGACCCCGA ATAAACTTGG CATCACGCGT TAAGTCgaca atcaacctct
1801ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)