Construct: ORF TRCN0000465875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008286.1_s317c1
- Derived from:
- ccsbBroadEn_12460
- DNA Barcode:
- TCTCCACTCTCTTGAGCCTGCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC1 (64215)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64215 | DNAJC1 | DnaJ heat shock protein fam... | NM_022365.4 | 61.3% | 61.1% | 1_642del;1555C>T |
| 2 | human | 64215 | DNAJC1 | DnaJ heat shock protein fam... | XM_011519614.3 | 31.1% | 28.1% | (many diffs) |
| 3 | human | 64215 | DNAJC1 | DnaJ heat shock protein fam... | XM_017016536.2 | 23.9% | 21.1% | (many diffs) |
| 4 | mouse | 13418 | Dnajc1 | DnaJ heat shock protein fam... | XM_011238947.1 | 75.6% | 69.7% | (many diffs) |
| 5 | mouse | 13418 | Dnajc1 | DnaJ heat shock protein fam... | NM_001190817.1 | 52.9% | 51.9% | (many diffs) |
| 6 | mouse | 13418 | Dnajc1 | DnaJ heat shock protein fam... | NM_007869.3 | 52.9% | 51.9% | (many diffs) |
| 7 | mouse | 13418 | Dnajc1 | DnaJ heat shock protein fam... | XR_865923.2 | 28.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa accacagtgg catgatttgc ttccatgcaa actggggatt tggttttgcc 121 ttacactaaa agcattacct cacctcatcc aggatgctgg gcagttttat gctaaatata 181 aagaaacaag attgaaggaa aaggaagatg cactgactag aactgaactt gaaacacttc 241 aaaaacagaa gaaagttaaa aaaccaaaac ctgaatttcc tgtatacaca cctttagaaa 301 ctacatatat tcagtcttat gatcatggaa cttccataga agaaattgag gaacaaatgg 361 atgattggtt ggaaaacagg aaccgaacac agaaaaaaca ggcacctgaa tggacagaag 421 aggacctcag ccaactgaca agaagtatgg ttaagttccc aggagggact ccaggtcgat 481 gggaaaagat tgcccacgaa ttgggtcgat ctgtgacaga tgtgacaacc aaagccaagc 541 aactgaagga ttcagtgacc tgctccccag gaatggttag actctccgaa ctcaaatcga 601 cagttcagaa ttccaggccc atcaaaacgg ccaccacctt gcccgatgac atgatcaccc 661 agcgagagga cgcagagggg gtggcagcgg aggaggagca ggagggagac tccggtgagc 721 aggagaccgg ggccactgat gcccggcctc ggaggcggaa gccagccagg ctgctggagg 781 ctacagcgaa gccggagcca gaggagaagt ccagagccaa gcggcagaag gactttgaca 841 tagcagaaca aaacgagtcc agcgacgagg agagcctgag aaaagagaga gctcggtctg 901 cagaggagcc gtggactcaa aatcaacaga aacttctgga actGGCGTTG CAGCAGTACC 961 CAAGGGGATC CTCTGACTGC TGGGACAAAA TAGCCAGATG TGTCCCGTCC AAGAGCAAGG 1021 AAGACTGTAT CGCTAGGTAC AAGTTGCTGG TTGAACTGGT CCAAAAGAAA AAACAAGCTA 1081 AAAGCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTCCACTC TCTTGAGCCT GCTCGACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt