Construct: ORF TRCN0000465876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012305.1_s317c1
- Derived from:
- ccsbBroadEn_03615
- DNA Barcode:
- GATCCTCACGCCACGCATGCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRBOX4 (55634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55634 | KRBOX4 | KRAB box domain containing 4 | NM_001129899.1 | 100% | 100% | |
| 2 | human | 55634 | KRBOX4 | KRAB box domain containing 4 | NM_001129900.1 | 94.8% | 94.8% | 235_236insCAGGTGAGGACAGGC |
| 3 | human | 55634 | KRBOX4 | KRAB box domain containing 4 | NM_017776.2 | 53.4% | 47.3% | (many diffs) |
| 4 | human | 55634 | KRBOX4 | KRAB box domain containing 4 | NM_001129898.2 | 51.2% | 46.7% | (many diffs) |
| 5 | human | 641339 | ZNF674 | zinc finger protein 674 | NM_001146291.2 | 15.9% | 13.7% | (many diffs) |
| 6 | human | 641339 | ZNF674 | zinc finger protein 674 | NM_001190417.2 | 15.8% | 13.5% | (many diffs) |
| 7 | human | 641339 | ZNF674 | zinc finger protein 674 | NM_001039891.3 | 15% | 13.8% | (many diffs) |
| 8 | human | 641339 | ZNF674 | zinc finger protein 674 | XM_011543941.3 | 15% | 13.8% | (many diffs) |
| 9 | human | 641339 | ZNF674 | zinc finger protein 674 | XM_011543943.3 | 14.9% | 13.6% | (many diffs) |
| 10 | human | 641339 | ZNF674 | zinc finger protein 674 | XM_011543944.2 | 8.9% | 7.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 384
- ORF length:
- 294
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCATGTC CCAGGAATCA TTGACCTTCA 121 AGGACGTGTT TGTGGACTTC ACCCTGGAGG AGTGGCAGCA ACTGGACTCT GCCCAGAAGA 181 ACCTCTACAG GGATGTCATG CTTGAGAACT ACAGCCACCT GGTGTCCGTG GGGTATCTAG 241 TTGCGAAGCC AGATGTGATC TTCAGGTTGG GACCAGGTGA AGAGTCCTGG ATGGCAGATG 301 GGGGGACCCC GGTACGGACC TGTGCAGGTG AGGACAGGCC AGATGTATCC ATTTTTGCCT 361 CATGTATTTT GAAGTGCTGT TATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG ATCCTCACGC 541 CACGCATGCA TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att