Transcript: Human XM_011543944.2

PREDICTED: Homo sapiens zinc finger protein 674 (ZNF674), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF674 (641339)
Length:
2247
CDS:
143..1780

Additional Resources:

NCBI RefSeq record:
XM_011543944.2
NBCI Gene record:
ZNF674 (641339)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011543944.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234948 ACTACGTCCAGCCTTATATAT pLKO_005 1058 CDS 100% 15.000 10.500 N ZNF674 n/a
2 TRCN0000234951 GAGAAGTCACCACTGATTAAA pLKO_005 1472 CDS 100% 15.000 10.500 N ZNF674 n/a
3 TRCN0000234952 TACTTGAGAGTGGGATAATTA pLKO_005 1784 3UTR 100% 15.000 10.500 N ZNF674 n/a
4 TRCN0000234950 ACACCTCTCTGTCCATCATAG pLKO_005 1312 CDS 100% 10.800 7.560 N ZNF674 n/a
5 TRCN0000234949 AGAAGCCCAGTCCCACTAAAC pLKO_005 1137 CDS 100% 10.800 7.560 N ZNF674 n/a
6 TRCN0000418910 ACCTTACAAATGTAGTGAATG pLKO_005 1603 CDS 100% 10.800 6.480 N Rex2 n/a
7 TRCN0000235219 ACAGGAGAGAAACCCTATAAA pLKO_005 1424 CDS 100% 15.000 7.500 Y LOC66376 n/a
8 TRCN0000235327 ACAGGAGAGAAACCCTATAAA pLKO_005 1424 CDS 100% 15.000 7.500 Y OTTMUSG00000016228 n/a
9 TRCN0000243738 CAGGAGAGAAACCCTATAAAT pLKO_005 1425 CDS 100% 15.000 7.500 Y Gm14430 n/a
10 TRCN0000262238 TACAGGAGAGAAACCCTATAA pLKO_005 1423 CDS 100% 13.200 6.600 Y Gm14305 n/a
11 TRCN0000239829 AGGAGAGAAACCCTATGAATG pLKO_005 1258 CDS 100% 10.800 5.400 Y Gm14393 n/a
12 TRCN0000284648 ATACAGGAGAGAAACCCTATA pLKO_005 1422 CDS 100% 10.800 5.400 Y Gm14308 n/a
13 TRCN0000414498 AGGACGTGTTTGTGGACTTCA pLKO_005 66 5UTR 100% 4.950 2.475 Y KRBOX4 n/a
14 TRCN0000284687 TACAGGAGAGAAACCTTATAA pLKO_005 1339 CDS 100% 15.000 7.500 Y Zfp984 n/a
15 TRCN0000239545 CCTTACAAATGTAGTGAATTT pLKO_005 1604 CDS 100% 13.200 6.600 Y Gm13212 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011543944.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03615 pDONR223 100% 8.9% 7.7% None (many diffs) n/a
2 ccsbBroad304_03615 pLX_304 0% 8.9% 7.7% V5 (many diffs) n/a
3 TRCN0000465876 GATCCTCACGCCACGCATGCATTA pLX_317 100% 8.9% 7.7% V5 (many diffs) n/a
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