Construct: ORF TRCN0000465883
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011558.1_s317c1
- Derived from:
- ccsbBroadEn_00562
- DNA Barcode:
- CTCAAGAGTACCAACAATTCGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPC5 (2262)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465883
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2262 | GPC5 | glypican 5 | NM_004466.6 | 100% | 100% | |
| 2 | human | 2262 | GPC5 | glypican 5 | XM_017020435.2 | 93.7% | 89.3% | (many diffs) |
| 3 | human | 2262 | GPC5 | glypican 5 | XM_011521054.3 | 83.1% | 82.3% | (many diffs) |
| 4 | human | 2262 | GPC5 | glypican 5 | XM_011521060.2 | 76.6% | 74.4% | (many diffs) |
| 5 | human | 2262 | GPC5 | glypican 5 | XM_011521055.3 | 76.5% | 75% | (many diffs) |
| 6 | human | 2262 | GPC5 | glypican 5 | XM_011521056.3 | 76.5% | 75% | (many diffs) |
| 7 | human | 2262 | GPC5 | glypican 5 | XM_011521057.3 | 76.5% | 75% | (many diffs) |
| 8 | human | 2262 | GPC5 | glypican 5 | XM_011521058.2 | 76.5% | 75% | (many diffs) |
| 9 | human | 2262 | GPC5 | glypican 5 | XM_011521059.2 | 76.5% | 75% | (many diffs) |
| 10 | human | 2262 | GPC5 | glypican 5 | XM_017020436.2 | 76.5% | 75% | (many diffs) |
| 11 | human | 2262 | GPC5 | glypican 5 | XM_017020437.1 | 76.5% | 75% | (many diffs) |
| 12 | mouse | 103978 | Gpc5 | glypican 5 | NM_175500.4 | 84.9% | 85.6% | (many diffs) |
| 13 | mouse | 103978 | Gpc5 | glypican 5 | XM_011244875.1 | 71.3% | 71.8% | (many diffs) |
| 14 | mouse | 103978 | Gpc5 | glypican 5 | XM_011244876.2 | 71.2% | 71.3% | (many diffs) |
| 15 | mouse | 103978 | Gpc5 | glypican 5 | XM_017315745.1 | 70.3% | 70.3% | (many diffs) |
| 16 | mouse | 103978 | Gpc5 | glypican 5 | XM_006518320.3 | 69.3% | 70.8% | (many diffs) |
| 17 | mouse | 103978 | Gpc5 | glypican 5 | XM_006518321.2 | 69.3% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgcacagacc tggcccgtgg gctttcgctg cctcctcctt ctggccctgg 121 ttgggtccgc ccgcagcgag ggcgtgcaga cctgcgaaga agttcggaaa cttttccagt 181 ggcggctgct gggagctgtc agggggctgc cggattcgcc gcgggcagga cctgatcttc 241 aggtttgcat atccaaaaag cctacatgtt gcaccaggaa gatggaggag agatatcaga 301 ttgcggctcg ccaggatatg cagcagtttc ttcaaacgtc cagctctaca ttaaagtttc 361 taatatctcg aaatgcggct gcttttcaag aaacccttga aactctcatc aaacaagcag 421 aaaattacac cagtatactt ttttgcagta cctacaggaa catggccttg gaggctgctg 481 cttcggttca ggagttcttc actgatgtgg ggctgtattt atttggtgcg gatgttaatc 541 ctgaagaatt tgtaaacaga ttttttgaca gtctttttcc tctggtctac aaccacctca 601 ttaaccctgg tgtgactgac agttccctgg aatactcaga atgcatccgg atggctcgcc 661 gggatgtgag tccatttggt aatattcccc aaagagtaat gggacagatg gggaggtccc 721 tgctgcccag ccgcactttt ctgcaggcac tcaatctggg cattgaagtc atcaacacca 781 cagactatct gcacttctcc aaagagtgca gcagagccct cctgaagatg caatactgcc 841 cgcactgcca aggcctggcg ctcactaagc cttgtatggg atactgcctc aatgtcatgc 901 gaggctgcct ggcgcacatg gcggagctta atccacactg gcatgcatat atccggtcgt 961 tggaagaact ctcggatgca atgcatggaa catacgacat tggacacgtg ctgctgaact 1021 ttcacttgct tgttaatgat gctgtgttac aggctcacct caatggacaa aaattattgg 1081 aacaggtaaa taggatttgt ggccgccctg taagaacacc cacacaaagc ccccgttgtt 1141 cttttgatca gagcaaagag aagcatggaa tgaagaccac cacaaggaac agtgaagaga 1201 cgcttgccaa cagaagaaaa gaatttatca acagccttcg actgtacagg tcattctatg 1261 gaggtctagc tgatcagctt tgtgctaatg aattagctgc tgcagatgga cttccctgct 1321 ggaatggaga agatatagta aaaagttata ctcagcgtgt ggttggaaat ggaatcaaag 1381 cccagtctgg aaatcctgaa gtcaaagtca aaggaattga tcctgtgata aatcagatta 1441 ttgataaact gaagcatgtt gttcagttgt tacagggtag aTCACCCAAA CCTGACAAGT 1501 GGGAACTTCT TCAGCTGGGC AGTGGTGGAG GCATGGTTGA ACAAGTCAGT GGGGACTGTG 1561 ATGATGAAGA TGGTTGCGGG GGATCAGGAA GTGGAGAAGT CAAGAGGACA CTGAAGATCA 1621 CAGACTGGAT GCCAGATGAT ATGAACTTCA GTGATGTAAA GCAAATCCAT CAAACAGACA 1681 CTGGCAGTAC TTTAGACACA ACAGGAGCAG GATGTGCAGT GGCGACTGAA TCTATGACAT 1741 TCACTCTGAT AAGTGTGGTG ATGTTACTTC CCGGGATTTG GTACCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGACTC AAGAGTACCA ACAATTCGGT TACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t