Construct: ORF TRCN0000465885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007442.1_s317c1
- Derived from:
- ccsbBroadEn_04365
- DNA Barcode:
- TCGTTTCTTTTATGTTATAGGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGAP4 (84302)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84302 | PGAP4 | post-GPI attachment to prot... | NM_001303107.2 | 99.9% | 100% | 54C>G |
| 2 | human | 84302 | PGAP4 | post-GPI attachment to prot... | NM_001303108.2 | 99.9% | 100% | 54C>G |
| 3 | human | 84302 | PGAP4 | post-GPI attachment to prot... | NM_001371233.1 | 99.9% | 100% | 54C>G |
| 4 | human | 84302 | PGAP4 | post-GPI attachment to prot... | NM_032342.3 | 99.9% | 100% | 54C>G |
| 5 | human | 84302 | PGAP4 | post-GPI attachment to prot... | XM_024447701.1 | 99.9% | 100% | 54C>G |
| 6 | mouse | 67063 | Tmem246 | transmembrane protein 246 | NM_025944.3 | 88.1% | 98.2% | (many diffs) |
| 7 | mouse | 67063 | Tmem246 | transmembrane protein 246 | XM_006538194.3 | 88.1% | 98.2% | (many diffs) |
| 8 | mouse | 67063 | Tmem246 | transmembrane protein 246 | XM_006538195.3 | 88.1% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cacttcaacc tctccagctg ccatgctcct ccggaggctg cggcgactgt 121 cctggggcag cactgctgtc cagctcttca tcctaacagt ggtgacgttt ggcctgctgg 181 cccccctggc ctgtcaccga cttctacact cttacttcta tctgcgccat tggcatctga 241 accaaatgag ccaagagttc ctgcagcaaa gcttgaaaga gggtgaggct gccctccact 301 attttgagga gcttccctct gccaatggct cagtgcccat tgtctggcag gccacccccc 361 ggccctggct ggtgatcacc atcatcactg tggacaggca gcctggcttc cactacgtcc 421 tgcaggttgt gtcccagttc caccggcttc ttcagcaatg tggcccccag tgcgaggggc 481 accaactctt cctgtgcaac gtggagcgta gtgtgagcca ttttgatgcc aagttgctct 541 ccaagtatgt ccctgtggcc aatcgctatg agggcactga ggatgattat ggtgatgacc 601 cttcgaccaa ctcgtttgag aaagagaagc aggactatgt ctattgcctg gagtcatccc 661 tgcagaccta caacccagac tacgtcctga tggtagaaga cgatgctgta ccagaagagc 721 agatcttccc agtcttggag caccttctgc gggctcgctt ctctgagcca catctcagag 781 atgcccttta tctcaagctg tatcaccccg agaggctcca gcactacatc aatccagagc 841 ccatgcggat cctggaatgg gttggtgtag gcatgttgct ggggccctta ctaacctGGA 901 TATACATGAG GTTTGCCAGC CGCCCAGGGT TTAGCTGGCC TGTAATGCTC TTCTTCTCCC 961 TGTATAGCAT GGGTCTGGTG GAGCTGGTGG GTCGGCACTA TTTCCTGGAA CTGCGGCGGC 1021 TGAGTCCTTC CCTGTACAGT GTGGTTCCTG CCTCTCAGTG TTGCACCCCA GCCATGCTCT 1081 TCCCGGCACC TGCGGCCCGC CGGACCCTCA CCTACCTGTC CCAAGTGTAC TGCCACAAGG 1141 GCTTTGGCAA GGACATGGCA CTGTACTCGC TGTTGAGGGC CAAGGGAGAG AGGGCCTATG 1201 TAGTGGAGCC GAACCTCGTG AAACACATCG GGCTCTTCTC CAGTCTCCGG TACAACTTTC 1261 ATCCCAGTCT CCTCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCGTTTCTTT TATGTTATAG 1441 GCAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt