Construct: ORF TRCN0000465896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005411.1_s317c1
- Derived from:
- ccsbBroadEn_15747
- DNA Barcode:
- TGGTACACAGTCATTTGCATACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMD4A (23034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_001161577.2 | 100% | 100% | |
| 2 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449519.1 | 61% | 61% | 1_660del |
| 3 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449518.1 | 52.8% | 52.8% | 1_924del |
| 4 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449517.1 | 51.8% | 51.8% | 1_963del |
| 5 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_001161576.2 | 46.3% | 46.3% | 1_963del;1778_1779ins108 |
| 6 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449514.1 | 45.7% | 45.7% | 1_1227del |
| 7 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449515.1 | 45.7% | 45.7% | 1_1227del |
| 8 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449516.1 | 42.8% | 42.8% | 1_1227del;2083_2084ins66 |
| 9 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_015589.6 | 40.9% | 40.9% | 1_1227del;2042_2043ins108 |
| 10 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001310544.1 | 78.8% | 82% | (many diffs) |
| 11 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519631.3 | 78.8% | 82% | (many diffs) |
| 12 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001163433.1 | 41.6% | 43.4% | (many diffs) |
| 13 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_028966.3 | 40.8% | 42.5% | (many diffs) |
| 14 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519628.2 | 38.7% | 40.3% | (many diffs) |
| 15 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001037221.2 | 36.1% | 37.6% | (many diffs) |
| 16 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519627.2 | 33.8% | 35.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1101
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cctgactccg atcaaggcct acagctcccc gagcaccacc cccgaggctc 121 gccgccggga gccccaggcc ccgcgtcagc cctcactgat gggccccgag agccagagcc 181 ccgactgcaa agatggggcc gcagccactg gcgccacggc caccccctcg gccggggcca 241 gcggggggct ccagccgcac cagctgagca gctgcgatgg ggagctggcc gtcgcccccc 301 tgccagaggg ggacctcccc gggcagttca cacgcgtcat ggggaaagtg tgcacacagc 361 tcttggtctc cagacctgat gaggaaaata taagttccta tttacagctc atagacaagt 421 gtctaattca tgaggcattt acagagacac agaaaaaaag attgttgtca tggaaacagc 481 aggtgcagaa gctctttcgg tctttccctc ggaaaaccct tctagacata tcaggatatc 541 gacagcaaag aaatcgaggc tttgggcaat ccaactccct cccgacggct ggctctgtgg 601 gcggtggcat gggcagacgg aacccgcgcc agtaccagat cccctctcgg aacgtccctt 661 ccgcccgcct gggcctcttg ggcaccagtg gattcGTCAG CTCCAACCAG CGCAACACCA 721 CAGCTACCCC CACCATCATG AAACAAGGAA GACAGAACCT GTGGTTTGCC AACCCCGGGG 781 GCAGCAATAG CATGCCAAGC CGCACCCACA GCTCAGTCCA GAGGACCCGC TCGCTGCCCG 841 TGCACACTTC CCCACAGAAC ATGCTGATGT TCCAGCAGCC AGGTTCCCAA GTTCACAGTG 901 GACTGTGTTT AACAGACCTT AGAGGTTGGT TGAGCCTGAG TGGAGCCCCT TCCATGCCAG 961 CGCTGACAGT CCCCAAGTCC TCCCAGGGAG AATTCCAGCT TCCCGTGACC GAACCTGACA 1021 TCAACAACAG GCTGGAGTCG TTGTGCCTCA GTATGACCGA ACACGCCCTG GGAGACGGGG 1081 TTGACCGGAC CTCCACCATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGGT ACACAGTCAT 1261 TTGCATACCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt