Construct: ORF TRCN0000465899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000797.1_s317c1
- Derived from:
- ccsbBroadEn_10157
- DNA Barcode:
- GAGAGTTCGCTCAGAAAAATCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHX8 (431707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 431707 | LHX8 | LIM homeobox 8 | NM_001001933.1 | 99.9% | 99.7% | 890T>C |
| 2 | human | 431707 | LHX8 | LIM homeobox 8 | NM_001256114.2 | 97% | 96.9% | 0_1ins30;860T>C |
| 3 | human | 431707 | LHX8 | LIM homeobox 8 | XM_017001316.1 | 88.3% | 86.4% | (many diffs) |
| 4 | human | 431707 | LHX8 | LIM homeobox 8 | XM_017001317.1 | 88.3% | 86.4% | (many diffs) |
| 5 | mouse | 16875 | Lhx8 | LIM homeobox protein 8 | XM_006501072.3 | 83.4% | 88.7% | (many diffs) |
| 6 | mouse | 16875 | Lhx8 | LIM homeobox protein 8 | XM_017319470.1 | 83.4% | 88.7% | (many diffs) |
| 7 | mouse | 16875 | Lhx8 | LIM homeobox protein 8 | NM_010713.2 | 80.4% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gattctgagc aggtgtcagg ggctcatgtc agaggagtgc gggcggacta 121 cagccctggc ggccgggagg actcgcaaag gcgccgggga agagggactg gtgagccccg 181 agggagcggg ggacgaggac tcgtgctcct cctcggcccc gctgtccccg tcgtcctcgc 241 cccggtccat ggcctcgggc tccggctgcc ctcctggcaa gtgtgtgtgc aacagttgcg 301 gcctggagat cgtggacaaa taccttctca aggtgaatga cctatgctgg catgtccggt 361 gtctctcctg cagtgtttgc agaacctccc taggaaggca caccagctgt tatattaaag 421 acaaagacat tttctgcaaa cttgattatt tcagaaggta tggaactcgc tgctctcgat 481 gtgggagaca catccattct actgactggg tccggagagc caaggggaat gtctatcact 541 tggcatgctt tgcctgcttt tcctgcaaaa ggcaactttc cacaggagag gagtttgctt 601 tggtggaaga gaaagtcctc tgcagagtac attatgactg catgctggat aatttaaaaa 661 gagaagtaga aaatgggaat gggattagtg tggaaggtgc cctcctcaca gagcaagatg 721 ttaaccatcc aaaaccagca aaaagagctc ggaccagctt tacagcagat cagcttcagg 781 ttatgcaagc acaatttgct caggacaaCA ACCCAGATGC ACAGACACTC CAGAAATTGG 841 CAGAAAGGAC AGGCTTGAGC AGACGTGTGA TACAGGTGTG GTTTCAGAAT TGTAGAGCAC 901 GCCACAAGAA ACACGTCAGT CCTAATCACT CATCCTCCAC CCCAGTCACA GCAGCCCCAC 961 CCTCCAGGCT GTCTCCACCC ATGTTAGAAG AAATGGCTTA TTCTGCCTAC GTGCCCCAAG 1021 ATGGAACGAT GTTAACTGCG CTGCATAGTT ATATGGATGC TCATTCACCA ACAACTCTTG 1081 GACTCCAGCC CTTGTTACCC CATTCAATGA CACAACTGCC AATAAGTCAT ACCTACCCAA 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAG AGAGTTCGCT CAGAAAAATC GGCACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att