Construct: ORF TRCN0000465958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004244.1_s317c1
- Derived from:
- ccsbBroadEn_14010
- DNA Barcode:
- AAGCTGAGTCGGGCCAGGGGTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSTPIP2 (9050)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465958
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9050 | PSTPIP2 | proline-serine-threonine ph... | XM_011526254.2 | 88.2% | 69% | (many diffs) |
| 2 | human | 9050 | PSTPIP2 | proline-serine-threonine ph... | NM_024430.4 | 87.5% | 67.8% | 4A>C;742_838del;1002_1003ins31 |
| 3 | human | 9050 | PSTPIP2 | proline-serine-threonine ph... | XM_011526253.2 | 79.9% | 60.6% | (many diffs) |
| 4 | human | 9050 | PSTPIP2 | proline-serine-threonine ph... | XM_011526256.1 | 69.2% | 49.6% | (many diffs) |
| 5 | human | 9050 | PSTPIP2 | proline-serine-threonine ph... | XR_935264.2 | 30.7% | (many diffs) | |
| 6 | mouse | 19201 | Pstpip2 | proline-serine-threonine ph... | NM_013831.4 | 75.1% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1002
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcgctcactg ttcaagggaa acttttggag tgcagacatc ctcagcacca 121 tcggctatga caacattatc caacatctga acaatggccg caagaactgc aaagagtttg 181 aagactttct aaaagaaagg gcagcaattg aagagaggta tggcaaagat ctgctcaacc 241 tctctaggaa gaagccgtgt ggacagtctg aaatcaacac cctgaagcgg gcccttgaag 301 tcttcaagca gcaagtagac aatgtggcac aatgtcacat tcagcttgca cagagtttaa 361 gagaagaggc caggaagatg gaagaattca gggaaaagca aaaactacaa cgaaaaaaga 421 cagagctcat aatggatgct atccataaac aaaagagctt acaattcaag aaaaccatgg 481 atgcaaagaa gaactatgag cagaaatgcc gggacaaaga tgaggcagaa caggccgtca 541 gccggagtgc caacctggtg aacccgaagc aacaagaaaa gctttttgtg aaactggcaa 601 cttcaaagac cgcagtagag gacTCAGACA AAGCATACAT GCTGCACATC GGCACCCTGG 661 ATAAGGTCCG AGAAGAGTGG CAGAGTGAGC ACATCAAGGC CTGCGAGGCA TTTGAGGCTC 721 AAGAATGTGA ACGAATAAAC TTCTTCCGGA ATGCATTGTG GTTACATGTG AATCAGCTGT 781 CACAACAATG TGTCACCAGT GATGAACACC CATCATGTAT GAGAATTTCT ACTCCTCCCA 841 GAAGAATGCA GTCCCAGCAG GAAAGGCTAC AGGGCCTAAC TTGGCAAGGA GAGGACCCCT 901 CCCAATTCCT AAAAGCTCAC CAGATGATCC CAATTACTCT TTGGTTGATG ACTACAGTTT 961 GCTCTATCAG TAAAATCAAT GAAACCAGAG CTTTTTCCGG CTACCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGAAAG CTGAGTCGGG CCAGGGGTGC CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t