Construct: ORF TRCN0000465960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013787.1_s317c1
- Derived from:
- ccsbBroadEn_09583
- DNA Barcode:
- GTAATTTTCATGTTATTGAATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UPRT (139596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139596 | UPRT | uracil phosphoribosyltransf... | NM_145052.4 | 99.8% | 100% | 381T>G |
| 2 | human | 139596 | UPRT | uracil phosphoribosyltransf... | NM_001307944.1 | 89.8% | 89.6% | (many diffs) |
| 3 | human | 139596 | UPRT | uracil phosphoribosyltransf... | NM_001363821.1 | 55.9% | 55.9% | 0_1ins408 |
| 4 | human | 139596 | UPRT | uracil phosphoribosyltransf... | XM_011530867.3 | 53.4% | 47.8% | (many diffs) |
| 5 | human | 139596 | UPRT | uracil phosphoribosyltransf... | NR_030774.1 | 36% | (many diffs) | |
| 6 | mouse | 331487 | Uprt | uracil phosphoribosyltransf... | NM_001081189.1 | 88.5% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacggagtta cagtgtccgg actccatgcc ctgtcacaac cagcaagtaa 121 actctgcctc aaccccaagt cccgagcagc tgcgacctgg cgatctgatc ctggaccacg 181 cagggggaaa cagagcctcc agggccaagg tgattctcct cacggggtac gcccattcta 241 gcctgccggc cgagctggac tctggggcct gcggcggctc cagcctcaac tcagagggca 301 acagtggtag tggtgacagt agcagctatg acgcaccagc tggcaactcc ttcctagagg 361 actgcgaact ctcccggcag atcggggcgc agcttaagct gctgcctatg aatgatcaga 421 tacgggagct acagaccatc atccgggaca agacagccag tagaggtgac ttcatgtttt 481 ctgcggatcg tttgatcaga cttgttgtgg aagagggatt gaatcagctg ccatataaag 541 aatgcatggt gaccactcca acagggtaca agtatgaagg agtgaaattt gagaagggaa 601 attgtggggt cagcataatg agaagcggtg aggcaatgga acaaggttta cgagactgct 661 gtcgatccat acgaattgga aagatccTGA TTCAGAGTGA TGAGGAGACA CAAAGAGCCA 721 AAGTATATTA TGCCAAATTC CCCCCAGACA TTTACCGGAG AAAAGTCCTT CTGATGTATC 781 CAATTCTCAG CACTGGAAAT ACTGTAATTG AAGCTGTAAA GGTTCTTATA GAACATGGAG 841 TTCAACCCAG TGTTATCATC CTACTCAGTC TGTTCTCCAC TCCTCATGGT GCCAAATCAA 901 TCATTCAGGA GTTTCCAGAG ATCACAATTT TAACTACTGA AGTTCATCCT GTTGCACCTA 961 CACATTTTGG ACAGAAATAC TTTGGAACAG ACTACCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG 1141 TAATTTTCAT GTTATTGAAT ACAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 1201 tttgtgaaag att