Transcript: Human NM_145052.4

Homo sapiens uracil phosphoribosyltransferase homolog (UPRT), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
UPRT (139596)
Length:
2474
CDS:
171..1100

Additional Resources:

NCBI RefSeq record:
NM_145052.4
NBCI Gene record:
UPRT (139596)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_145052.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425135 GCTGTCGATCCATACGAATTG pLKO_005 763 CDS 100% 10.800 15.120 N UPRT n/a
2 TRCN0000431473 GAGGCAATGGAACAAGGTTTA pLKO_005 735 CDS 100% 10.800 8.640 N UPRT n/a
3 TRCN0000151616 GACACAAAGAGCCAAAGTATA pLKO.1 812 CDS 100% 13.200 9.240 N UPRT n/a
4 TRCN0000423574 GCACTGGAAATACTGTAATTG pLKO_005 895 CDS 100% 13.200 9.240 N UPRT n/a
5 TRCN0000413187 ATGGTGCCAAATCAATCATTC pLKO_005 991 CDS 100% 10.800 7.560 N UPRT n/a
6 TRCN0000421399 TGCCTATGAATGATCAGATAC pLKO_005 508 CDS 100% 10.800 7.560 N UPRT n/a
7 TRCN0000155595 CCTGTCACAACCAGCAAGTAA pLKO.1 205 CDS 100% 5.625 3.938 N UPRT n/a
8 TRCN0000446179 ACCACTCCAACAGGGTACAAG pLKO_005 657 CDS 100% 4.950 3.465 N UPRT n/a
9 TRCN0000151681 GATCGTTTGATCAGACTTGTT pLKO.1 591 CDS 100% 4.950 3.465 N UPRT n/a
10 TRCN0000154977 GCGGATCGTTTGATCAGACTT pLKO.1 588 CDS 100% 4.950 3.465 N UPRT n/a
11 TRCN0000150589 GCTCTACTAAGGTTTCACATA pLKO.1 1484 3UTR 100% 4.950 3.465 N UPRT n/a
12 TRCN0000154809 GCTGGATTTCATGGCCAGTAA pLKO.1 1901 3UTR 100% 4.950 3.465 N UPRT n/a
13 TRCN0000151418 GTACAAGTATGAAGGAGTGAA pLKO.1 671 CDS 100% 4.950 3.465 N UPRT n/a
14 TRCN0000423196 GTTCAACCCAGTGTTATCATC pLKO_005 945 CDS 100% 4.950 2.970 N UPRT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145052.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09583 pDONR223 100% 99.8% 100% None 381T>G n/a
2 ccsbBroad304_09583 pLX_304 0% 99.8% 100% V5 381T>G n/a
3 TRCN0000465960 GTAATTTTCATGTTATTGAATACA pLX_317 33.3% 99.8% 100% V5 381T>G n/a
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