Construct: ORF TRCN0000465970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001117.1_s317c1
- Derived from:
- ccsbBroadEn_13535
- DNA Barcode:
- AAATCCTAGTAACGCCCCAATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIMKLA (284716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465970
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284716 | RIMKLA | ribosomal modification prot... | NM_173642.4 | 89.4% | 89.5% | 1_123del;1047T>C |
| 2 | human | 284716 | RIMKLA | ribosomal modification prot... | XM_006710585.3 | 45.7% | 40% | 1_123del;569_570ins116;660_661ins397 |
| 3 | mouse | 194237 | Rimkla | ribosomal modification prot... | NM_177572.4 | 75.4% | 78.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccagatcgcc gtcaccatcg tcggcggcca cctcggcctc cagctaaacc 121 agaaggccct caccactttc ccggatgtgg tgcttgtacg ggtacccaca ccctcagtgc 181 agtcagacag tgacatcact gtcctgcgac acctggagaa gctgggctgc cggttggtca 241 atcgcccaca gagcatctta aattgcatca acaaattctg gacgttccaa gaactggctg 301 gacatggggt ccccatgcca gacaccttct cctatggtgg gcatgaagac ttttcaaaaa 361 tgattgatga agctgagccc ctgggctacc cagtcgtggt gaagagcaca cgaggccacc 421 ggggaaaagc tgtttttctg gcaagagata aacatcacct ctctgacatc tgccatctga 481 tccgccacga tgtgccctac ctgttccaga agtacgtgaa ggagtcccat ggaaaggaca 541 tccgggtggt ggtggtaggg ggccaggtca taggctctat gcttcgctgc tccactgatg 601 gacggatgca gagcaactgc tctctcggtg gcgtgggcgt caagtgtccg ctgacagaac 661 aaggcaagca gttggctatt caggtgtcca acatcctagg catggacttc tgtggcattg 721 aTCTCCTTAT CATGGACGAT GGCTCCTTTG TGGTGTGTGA GGCAAATGCT AATGTTGGCT 781 TCCTAGCCTT TGACCAGGCA TGCAACTTAG ATGTGGGTGG GATCATTGCA GACTATACCA 841 TGTCCTTGCT GCCAAATAGG CAGACTGGAA AGATGGCTGT CCTCCCAGGA CTGTCGAGTC 901 CAAGGGAGAA GAACGAGCCG GATGGCTGTG CTTCAGCTCA GGGAGTTGCA GAGAGCGTCT 961 ATACCATCAA CAGTGGGTCT ACCTCTAGCG AAAGTGAGCC TGAACTGGGA GAGATCCGGG 1021 ATTCCTCAGC AAGCACAATG GGGGCCCCAC CCTCCATGCT GCCCGAACCT GGCTACAACA 1081 TTAACAACAG GATTGCTTCT GAGTTAAAAC TTAAGTGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 AAAATCCTAG TAACGCCCCA ATATAACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt