Construct: ORF TRCN0000465987

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003075.1_s317c1
Derived from:
ccsbBroadEn_04607
DNA Barcode:
CAAGAGCCGTGAGACGTTCAGATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TBCK (93627)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465987
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 93627 TBCK TBC1 domain containing kinase NM_001163437.3 99.9% 99.8% 679C>G
2 human 93627 TBCK TBC1 domain containing kinase NM_001163435.3 95.5% 95.5% 480_596del;796C>G
3 human 93627 TBCK TBC1 domain containing kinase NM_001163436.4 95.5% 95.5% 480_596del;796C>G
4 human 93627 TBCK TBC1 domain containing kinase XM_011532417.2 95.5% 95.5% 480_596del;796C>G
5 human 93627 TBCK TBC1 domain containing kinase XM_017008846.1 95.5% 95.5% 480_596del;796C>G
6 human 93627 TBCK TBC1 domain containing kinase XM_024454281.1 95.5% 95.5% 480_596del;796C>G
7 human 93627 TBCK TBC1 domain containing kinase XM_024454282.1 95.5% 95.5% 480_596del;796C>G
8 human 93627 TBCK TBC1 domain containing kinase NM_033115.5 94.6% 88.3% (many diffs)
9 human 93627 TBCK TBC1 domain containing kinase XM_017008847.2 91.5% 91.4% 480_596del;796C>G;2571_2572ins108
10 human 93627 TBCK TBC1 domain containing kinase XM_017008848.1 83.7% 83.6% 0_1ins318;162_278del;478C>G
11 human 93627 TBCK TBC1 domain containing kinase NM_001290768.2 83.3% 80% (many diffs)
12 human 93627 TBCK TBC1 domain containing kinase XM_017008849.1 83.3% 80% (many diffs)
13 human 93627 TBCK TBC1 domain containing kinase XR_001741354.2 75.2% (many diffs)
14 human 93627 TBCK TBC1 domain containing kinase XR_938800.3 73.7% (many diffs)
15 human 93627 TBCK TBC1 domain containing kinase XR_001741353.2 72.4% (many diffs)
16 human 93627 TBCK TBC1 domain containing kinase XR_002959772.1 28.9% (many diffs)
17 mouse 271981 Tbck TBC1 domain containing kinase NM_001163455.2 85.1% 91.3% (many diffs)
18 mouse 271981 Tbck TBC1 domain containing kinase XM_030252639.1 71.3% 76.3% (many diffs)
19 mouse 271981 Tbck TBC1 domain containing kinase XM_006501553.4 68.3% 70% (many diffs)
20 mouse 271981 Tbck TBC1 domain containing kinase XR_001783696.2 34.2% (many diffs)
21 mouse 271981 Tbck TBC1 domain containing kinase XR_003954329.1 32.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2628
ORF length:
2562
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt tcccctgaag gacgctgaaa tgggagcctt taccttcttt gcctcggctc
121tgccacatga tgtttgtgga agcaatggac ttcctctcac accaaattcc atcaaaattt
181tagggcgctt tcaaatcctt aaaaccatca cccatcccag actctgccag tatgtggata
241tttctagggg aaagcatgaa cgactagtgg tcgtggctga acattgtgaa cgtagtctgg
301aagacttgct tcgagaaagg aaacctgtga gctgttcaac ggttttgtgt atagcatttg
361aggttcttca gggcttgcag tatatgaaca aacatggtat agtacacagg gcattgtctc
421ctcataatat cctgttggac cgaaagggac atattaaatt ggctaaattt ggactttatc
481acatgacagc tcatggtgat gatgttgatt tcccaatagg gtatccctcg tacttggccc
541ctgagggaag aaaattattt cagagcttgg atatttctga aagactaaaa tttttgctta
601ctttggattg tgtagatgac actttaatag ttctggctga agagcatggt tgtttggaca
661ttataaagga gcttcctgaa actgtgatag atcttttgaa taagtgcctt accttccatc
721cttctaagag gccaacccca gatgaattaa tgaaggacaa agtattcagt gaggtatcac
781ctttatatac cccctttacc aaacctgcca gtctgttttc atcttctctg agatgtgctg
841atttaactct gcctgaggat atcagtcagt tgtgtaaaga tataaataat gattacctgg
901cagaaagatc tattgaagaa gtgtattacc tttggtgttt ggctggaggt gacttggaga
961aagagcttgt caacaaggaa atcattcgat ccaaaccacc tatctgcaca ctccccaatt
1021ttctctttga ggatggtgaa agctttggac aaggtcgaga tagaagctcg cttttagatg
1081ataccactgt gacattgtcg ttatgccagc taagaaatag attgaaagat gttggtggag
1141aagcatttta cccattactt gaagatgacc agtctaattt acctcattca aacagcaata
1201atgagttgtc tgcagctgcc acgctccctt taatcatcag agagaaggat acagagtacc
1261aactaaatag aattattctc ttcgacaggc tgctaaaggc ttatccatat aaaaaaaacc
1321aaatctggaa agaagcaaga gttgacattc ctcctcttat gagaggttta acctgggctg
1381ctcttctggg agttgaggga gctattcatg ccaagtacga tgcaattgat aaagacactc
1441caattcctac agatagacaa attgaagtgg atattcctcg ctgtcatcag tacgatgaac
1501tgttatcatc accagaaggt catgcaaaat ttaggcgtgt attaaaagcc tgggtagtgt
1561ctcatcctga tcttgtgtat tggcaaggtc ttgactcact ttgtgctcca ttcctatatc
1621taaacttcaa taatgaagcc ttggcttatg catgtatgtc tgcttttatt cccaaatacc
1681tgtataactt cttcttaaaa gacaactcac atgtaataca agagtatctg actgtcttct
1741ctcagatgat tgcatttcat gatccagagc tgagtaatca tctcaatgag attggtttca
1801ttccagatct ctatgccatc ccttggtttc ttaccatgtt tactcatgta tttccactac
1861acaaaatttt ccacctctgg gataccttac tacttgggaa ttcctctttc ccattctgta
1921ttggagtagc aattcttcag cagctgcggg accggctttt ggctaatggc tttaatgagt
1981gtattcttct cttctccgat ttaccagaaa ttgacattga acgctgtgtg agagaatcta
2041tcaacctgtt ttgttggact cctaaaagtg ctacttacag acagcatgct caacctccaa
2101agccatcttc tgacagcagt ggaggcagaa gttcggcacc ttatttctct gctgagtgtc
2161cagatcctcc aaagacagat ctgtcaagag aatccatccc attaaatgac ctgaagtcag
2221aagtatcacc acggatttcA GCAGAGGACC TGATTGACTT GTGTGAGCTC ACAGTGACAG
2281GCCACTTCAA AACACCCAGC AAGAAAACAA AGTCCAGTAA ACCAAAGCTC CTGGTGGTTG
2341ACATCCGGAA TAGTGAAGAC TTTATTCGTG GTCACATTTC AGGAAGCATC AACATTCCAT
2401TCAGTGCTGC CTTCACTGCA GAAGGGGAGC TTACCCAGGG CCCTTACACT GCTATGCTCC
2461AGAACTTCAA AGGGAAGGTC ATTGTCATCG TGGGGCATGT GGCAAAACAC ACAGCTGAGT
2521TTGCAGCTCA CCTTGTGAAG ATGAAATATC CAAGAATCTG TATTCTAGAT GGTGGCATTA
2581ATAAAATAAA GCCAACAGGC CTCCTCACCA TCCCATCTCC TCAAATATGC CCAACTTTCT
2641TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2701CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2761GTGGAAAGGA CGACAAGAGC CGTGAGACGT TCAGATCACG CGTTAAGTCg acaatcaacc
2821tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)