Construct: ORF TRCN0000465993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004054.1_s317c1
- Derived from:
- ccsbBroadEn_02602
- DNA Barcode:
- AGCGACTTCACATGTCTCCTGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSTN (11034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465993
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11034 | DSTN | destrin, actin depolymerizi... | NM_006870.4 | 100% | 100% | |
| 2 | human | 11034 | DSTN | destrin, actin depolymerizi... | NM_001011546.2 | 89.6% | 89.6% | 0_1ins51 |
| 3 | human | 11034 | DSTN | destrin, actin depolymerizi... | XM_011529144.1 | 89.6% | 89.6% | 0_1ins51 |
| 4 | human | 171220 | DSTNP2 | DSTN pseudogene 2 | NR_033796.2 | 40.2% | (many diffs) | |
| 5 | mouse | 56431 | Dstn | destrin | NM_019771.2 | 90.7% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcaggagtg caagtagctg atgaagtatg tcgcattttt tatgacatga 121 aagttcgtaa atgctccaca ccagaagaaa tcaagaaaag aaagaaggct gtcatttttt 181 gtctcagtgc agacaaaaag tgcatcattg tagaagaagg caaagagatc ttggttggag 241 atgttggtgt aaccataact gaTCCTTTCA AGCATTTTGT GGGAATGCTT CCTGAAAAAG 301 ATTGTCGCTA TGCTTTGTAT GATGCAAGCT TTGAAACAAA AGAATCCAGA AAAGAAGAGT 361 TGATGTTTTT TTTGTGGGCA CCAGAACTAG CACCTCTGAA AAGTAAAATG ATCTATGCAA 421 GCTCCAAGGA TGCAATTAAA AAGAAATTTC AAGGCATAAA ACATGAATGT CAAGCAAATG 481 GACCAGAAGA TCTCAATCGG GCTTGTATTG CTGAAAAGTT AGGTGGATCC TTAATTGTAG 541 CCTTTGAAGG ATGCCCTGTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCG ACTTCACATG 721 TCTCCTGTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt