Construct: ORF TRCN0000466000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005935.1_s317c1
- Derived from:
- ccsbBroadEn_06052
- DNA Barcode:
- TAACGGGGAACGTTCGTTGATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSHL1 (1444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001318.4 | 99.4% | 99.2% | 135C>T;141C>A |
| 2 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022580.3 | 91.6% | 91.3% | 1_33del;168C>T;174C>A |
| 3 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321068.2 | 79.5% | 79.3% | 1_96del;231C>T;237C>A |
| 4 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321067.2 | 78.6% | 78.3% | 1_102del;237C>T;243C>A |
| 5 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321069.2 | 69.5% | 69.3% | 1_165del;300C>T;306C>A |
| 6 | human | 2688 | GH1 | growth hormone 1 | NM_022560.4 | 64.7% | 57.6% | (many diffs) |
| 7 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022581.3 | 63.9% | 63.8% | 1_213del;348C>T;354C>A |
| 8 | human | 2688 | GH1 | growth hormone 1 | NM_022559.4 | 57.7% | 52.9% | (many diffs) |
| 9 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022579.3 | 57.3% | 57.2% | 1_282del;417C>T;423C>A |
| 10 | human | 2689 | GH2 | growth hormone 2 | NM_022556.4 | 56.9% | 49% | (many diffs) |
| 11 | human | 1442 | CSH1 | chorionic somatomammotropin... | NM_001317.6 | 56.6% | 54.3% | (many diffs) |
| 12 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_020991.3 | 56.2% | 54.3% | (many diffs) |
| 13 | human | 2688 | GH1 | growth hormone 1 | NM_000515.5 | 53.7% | 49.3% | (many diffs) |
| 14 | human | 2689 | GH2 | growth hormone 2 | NM_002059.5 | 52.9% | 45.6% | (many diffs) |
| 15 | human | 2689 | GH2 | growth hormone 2 | NM_022558.4 | 46.2% | 20.9% | (many diffs) |
| 16 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022644.3 | 30.3% | 27.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 453
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggaaacg cagcagaaat ccaacttaga gctgctccac atctcccTGC 121 TGCTCATCGA GTCGCGGCTG GAGCCCGTGC GGTTCCTCAG GAGTACCTTC ACCAACAACC 181 TGGTGTATGA CACCTCGGAC AGTGATGAAT ATCACCTCCT AAAGGACCTA GAGGAAGGCA 241 TCCAAATGCT GATGGGGAGG CTGGAAGACG GCAGCCACCT GACTGGGCAG ACCCTCAAGC 301 AGACCTACAG CAAGTTTGAC ACAAACTCGC ACAACCATGA CGCACTGCTC AAGAACTACG 361 GGCTGCTCCA CTGCTTCAGG AAGGACATGG ACAAGGTCGA GACATTCCTG CGCATGGTGC 421 AGTGCCGCTC TGTGGAGGGC AGCTGTGGCT TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 601 ACGGGGAACG TTCGTTGATA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt