Transcript: Human NM_002059.5

Homo sapiens growth hormone 2 (GH2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
GH2 (2689)
Length:
903
CDS:
144..797

Additional Resources:

NCBI RefSeq record:
NM_002059.5
NBCI Gene record:
GH2 (2689)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002059.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000058227 GCAACGTCTATCGCCACCTGA pLKO.1 544 CDS 100% 0.880 1.232 N GH2 n/a
2 TRCN0000372359 ATATGACACCTATCAGGAGTT pLKO_005 293 CDS 100% 4.050 3.240 N GH2 n/a
3 TRCN0000372305 CGCCTGTACCAGCTGGCATAT pLKO_005 276 CDS 100% 3.600 2.880 N GH2 n/a
4 TRCN0000058223 ACACCTTCCAACAGGGTGAAA pLKO.1 399 CDS 100% 4.950 3.465 N GH2 n/a
5 TRCN0000058225 CTATATCCTGAAGGAGCAGAA pLKO.1 323 CDS 100% 4.050 2.835 N GH2 n/a
6 TRCN0000372358 TGAAGAAGCCTATATCCTGAA pLKO_005 314 CDS 100% 4.050 2.835 N GH2 n/a
7 TRCN0000058224 CAGCTGGCATATGACACCTAT pLKO.1 285 CDS 100% 0.000 0.000 N GH2 n/a
8 TRCN0000372306 TGGGCAGATCTTCAATCAGTC pLKO_005 626 CDS 100% 4.050 2.430 N GH2 n/a
9 TRCN0000378702 GAGTTTGAAGAAGCCTATATC pLKO_005 309 CDS 100% 13.200 6.600 Y GH1 n/a
10 TRCN0000377769 ATGGACAAGGTCGAGACATTC pLKO_005 729 CDS 100% 10.800 5.400 Y CSH1 n/a
11 TRCN0000372304 CTTCCCAACCATTCCCTTATC pLKO_005 221 CDS 100% 10.800 5.400 Y GH1 n/a
12 TRCN0000432549 ACATGGACAAGGTCGAGACAT pLKO_005 727 CDS 100% 4.950 2.475 Y CSHL1 n/a
13 TRCN0000372356 ACCTAGAGGAAGGCATCCAAA pLKO_005 568 CDS 100% 4.950 2.475 Y GH1 n/a
14 TRCN0000166034 CATGGACAAGGTCGAGACATT pLKO.1 728 CDS 100% 4.950 2.475 Y CSH2 n/a
15 TRCN0000083816 CTACAGCAAGTTTGACACAAA pLKO.1 647 CDS 100% 4.950 2.475 Y CSH1 n/a
16 TRCN0000415757 ACGCACTGCTCAAGAACTACG pLKO_005 682 CDS 100% 4.050 2.025 Y CSHL1 n/a
17 TRCN0000058226 CCATTCCCTTATCCAGGCTTT pLKO.1 229 CDS 100% 4.050 2.025 Y GH2 n/a
18 TRCN0000160300 CAGAAGTATTCATTCCTGCAT pLKO.1 339 CDS 100% 2.640 1.320 Y CSH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002059.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00634 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00634 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478654 CGGCTAGGGTAGCTGAACACGAGT pLX_317 45.7% 100% 100% V5 n/a
4 TRCN0000491319 GCTCCGACTGCGGGTACAATAATC pLX_317 36.4% 94.6% 93% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_06051 pDONR223 100% 90.6% 79.7% None (many diffs) n/a
6 ccsbBroad304_06051 pLX_304 0% 90.6% 79.7% V5 (many diffs) n/a
7 TRCN0000478974 TTCTAGGTATACTATTGCAGTGAG pLX_317 59.7% 90.6% 79.7% V5 (many diffs) n/a
8 ccsbBroadEn_00373 pDONR223 100% 90.4% 79.7% None (many diffs) n/a
9 ccsbBroad304_00373 pLX_304 0% 90.4% 79.7% V5 (many diffs) n/a
10 TRCN0000469020 CTTCGCCCGGCGTACGAATCACCC pLX_317 54.8% 90.4% 79.7% V5 (many diffs) n/a
11 ccsbBroadEn_15390 pDONR223 0% 90.3% 79.7% None (many diffs) n/a
12 ccsbBroad304_15390 pLX_304 0% 90.3% 79.7% V5 (many diffs) n/a
13 TRCN0000478638 GCAGCTCCTCTCTGGAGCAATCTC pLX_317 59.7% 90.3% 79.7% V5 (many diffs) n/a
14 ccsbBroadEn_00374 pDONR223 100% 90.1% 80.1% None (many diffs) n/a
15 ccsbBroad304_00374 pLX_304 0% 90.1% 80.1% V5 (many diffs) n/a
16 TRCN0000466754 AAACTGCTGAAATAACCAGGGAGG pLX_317 61.2% 90.1% 80.1% V5 (many diffs) n/a
17 ccsbBroadEn_00375 pDONR223 100% 53.3% 45.6% None (many diffs) n/a
18 ccsbBroad304_00375 pLX_304 0% 53.3% 45.6% V5 (many diffs) n/a
19 TRCN0000475609 ATAACCGATCTTCGAGTCTGGGAC pLX_317 61.6% 53.3% 45.6% V5 (many diffs) n/a
20 ccsbBroadEn_06052 pDONR223 100% 52.9% 45.6% None (many diffs) n/a
21 ccsbBroad304_06052 pLX_304 0% 52.9% 45.6% V5 (many diffs) n/a
22 TRCN0000466000 TAACGGGGAACGTTCGTTGATAAT pLX_317 85% 52.9% 45.6% V5 (many diffs) n/a
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