Transcript: Human NM_022644.3

Homo sapiens chorionic somatomammotropin hormone 2 (CSH2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
CSH2 (1443)
Length:
1136
CDS:
116..619

Additional Resources:

NCBI RefSeq record:
NM_022644.3
NBCI Gene record:
CSH2 (1443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_022644.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000371925 TTCTGCTTCTCAGACTCTATT pLKO_005 347 CDS 100% 13.200 6.600 Y CSH1 n/a
2 TRCN0000083815 AGGAGTTTGAAGAAACCTATA pLKO.1 279 CDS 100% 10.800 5.400 Y CSH1 n/a
3 TRCN0000377769 ATGGACAAGGTCGAGACATTC pLKO_005 954 3UTR 100% 10.800 5.400 Y CSH1 n/a
4 TRCN0000155751 CGATGACTATCACCTCCTAAA pLKO.1 517 CDS 100% 10.800 5.400 Y CSHL1 n/a
5 TRCN0000371980 TCGCCAACAACCTGGTGTATG pLKO_005 483 CDS 100% 10.800 5.400 Y CSH1 n/a
6 TRCN0000432549 ACATGGACAAGGTCGAGACAT pLKO_005 952 3UTR 100% 4.950 2.475 Y CSHL1 n/a
7 TRCN0000372356 ACCTAGAGGAAGGCATCCAAA pLKO_005 540 CDS 100% 4.950 2.475 Y GH1 n/a
8 TRCN0000157795 CAAGCAGACCTACAGCAAGTT pLKO.1 863 3UTR 100% 4.950 2.475 Y CSHL1 n/a
9 TRCN0000164750 CAGGAGTATGTTCGCCAACAA pLKO.1 472 CDS 100% 4.950 2.475 Y CSH2 n/a
10 TRCN0000083814 CAGGAGTTTGAAGAAACCTAT pLKO.1 278 CDS 100% 4.950 2.475 Y CSH1 n/a
11 TRCN0000158524 CAGGAGTTTGAAGAAACCTAT pLKO.1 278 CDS 100% 4.950 2.475 Y CSH2 n/a
12 TRCN0000166034 CATGGACAAGGTCGAGACATT pLKO.1 953 3UTR 100% 4.950 2.475 Y CSH2 n/a
13 TRCN0000166199 CATGGAGGAAACGCAACAGAA pLKO.1 382 CDS 100% 4.950 2.475 Y CSH2 n/a
14 TRCN0000083816 CTACAGCAAGTTTGACACAAA pLKO.1 872 3UTR 100% 4.950 2.475 Y CSH1 n/a
15 TRCN0000083817 GAAACGCAACAGAAATCCAAT pLKO.1 389 CDS 100% 4.950 2.475 Y CSH1 n/a
16 TRCN0000158937 GAAACGCAACAGAAATCCAAT pLKO.1 389 CDS 100% 4.950 2.475 Y CSH2 n/a
17 TRCN0000166095 GCAGACCTACAGCAAGTTTGA pLKO.1 866 3UTR 100% 4.950 2.475 Y CSH2 n/a
18 TRCN0000083813 GCGATGACTATCACCTCCTAA pLKO.1 516 CDS 100% 4.950 2.475 Y CSH1 n/a
19 TRCN0000156711 GCGATGACTATCACCTCCTAA pLKO.1 516 CDS 100% 4.950 2.475 Y CSHL1 n/a
20 TRCN0000415757 ACGCACTGCTCAAGAACTACG pLKO_005 907 3UTR 100% 4.050 2.025 Y CSHL1 n/a
21 TRCN0000162330 CTATATCCCAAAGGACCAGAA pLKO.1 295 CDS 100% 4.050 2.025 Y CSH2 n/a
22 TRCN0000160300 CAGAAGTATTCATTCCTGCAT pLKO.1 311 CDS 100% 2.640 1.320 Y CSH2 n/a
23 TRCN0000162110 CCAGAAGTATTCATTCCTGCA pLKO.1 310 CDS 100% 2.160 1.080 Y CSH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022644.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15390 pDONR223 0% 74.5% 71.1% None (many diffs) n/a
2 ccsbBroad304_15390 pLX_304 0% 74.5% 71.1% V5 (many diffs) n/a
3 TRCN0000478638 GCAGCTCCTCTCTGGAGCAATCTC pLX_317 59.7% 74.5% 71.1% V5 (many diffs) n/a
4 ccsbBroadEn_00373 pDONR223 100% 74.5% 71.1% None (many diffs) n/a
5 ccsbBroad304_00373 pLX_304 0% 74.5% 71.1% V5 (many diffs) n/a
6 TRCN0000469020 CTTCGCCCGGCGTACGAATCACCC pLX_317 54.8% 74.5% 71.1% V5 (many diffs) n/a
7 ccsbBroadEn_06051 pDONR223 100% 74.3% 71.1% None (many diffs) n/a
8 ccsbBroad304_06051 pLX_304 0% 74.3% 71.1% V5 (many diffs) n/a
9 TRCN0000478974 TTCTAGGTATACTATTGCAGTGAG pLX_317 59.7% 74.3% 71.1% V5 (many diffs) n/a
10 ccsbBroadEn_00374 pDONR223 100% 74.1% 71.5% None (many diffs) n/a
11 ccsbBroad304_00374 pLX_304 0% 74.1% 71.5% V5 (many diffs) n/a
12 TRCN0000466754 AAACTGCTGAAATAACCAGGGAGG pLX_317 61.2% 74.1% 71.5% V5 (many diffs) n/a
13 ccsbBroadEn_00634 pDONR223 100% 66.3% 54.5% None (many diffs) n/a
14 ccsbBroad304_00634 pLX_304 0% 66.3% 54.5% V5 (many diffs) n/a
15 TRCN0000478654 CGGCTAGGGTAGCTGAACACGAGT pLX_317 45.7% 66.3% 54.5% V5 (many diffs) n/a
16 TRCN0000491319 GCTCCGACTGCGGGTACAATAATC pLX_317 36.4% 64.4% 58.2% V5 (not translated due to prior stop codon) (many diffs) n/a
17 ccsbBroadEn_00375 pDONR223 100% 30.6% 28.4% None (many diffs) n/a
18 ccsbBroad304_00375 pLX_304 0% 30.6% 28.4% V5 (many diffs) n/a
19 TRCN0000475609 ATAACCGATCTTCGAGTCTGGGAC pLX_317 61.6% 30.6% 28.4% V5 (many diffs) n/a
20 ccsbBroadEn_06052 pDONR223 100% 30.3% 27.9% None (many diffs) n/a
21 ccsbBroad304_06052 pLX_304 0% 30.3% 27.9% V5 (many diffs) n/a
22 TRCN0000466000 TAACGGGGAACGTTCGTTGATAAT pLX_317 85% 30.3% 27.9% V5 (many diffs) n/a
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