Transcript: Human NM_000515.5

Homo sapiens growth hormone 1 (GH1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
GH1 (2688)
Length:
823
CDS:
64..717

Additional Resources:

NCBI RefSeq record:
NM_000515.5
NBCI Gene record:
GH1 (2688)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000515.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000058533 GCAACGTCTATGACCTCCTAA pLKO.1 464 CDS 100% 4.950 6.930 N GH1 n/a
2 TRCN0000058534 CTACAGCAAGTTCGACACAAA pLKO.1 567 CDS 100% 4.950 2.970 N GH1 n/a
3 TRCN0000058537 GTTCGACACAAACTCACACAA pLKO.1 576 CDS 100% 4.950 2.970 N GH1 n/a
4 TRCN0000058536 GCAGATCTTCAAGCAGACCTA pLKO.1 549 CDS 100% 2.640 1.584 N GH1 n/a
5 TRCN0000058535 CCTCTGTTTCTCAGAGTCTAT pLKO.1 294 CDS 100% 0.495 0.297 N GH1 n/a
6 TRCN0000378702 GAGTTTGAAGAAGCCTATATC pLKO_005 229 CDS 100% 13.200 6.600 Y GH1 n/a
7 TRCN0000377769 ATGGACAAGGTCGAGACATTC pLKO_005 649 CDS 100% 10.800 5.400 Y CSH1 n/a
8 TRCN0000372304 CTTCCCAACCATTCCCTTATC pLKO_005 141 CDS 100% 10.800 5.400 Y GH1 n/a
9 TRCN0000432549 ACATGGACAAGGTCGAGACAT pLKO_005 647 CDS 100% 4.950 2.475 Y CSHL1 n/a
10 TRCN0000372356 ACCTAGAGGAAGGCATCCAAA pLKO_005 488 CDS 100% 4.950 2.475 Y GH1 n/a
11 TRCN0000157795 CAAGCAGACCTACAGCAAGTT pLKO.1 558 CDS 100% 4.950 2.475 Y CSHL1 n/a
12 TRCN0000166034 CATGGACAAGGTCGAGACATT pLKO.1 648 CDS 100% 4.950 2.475 Y CSH2 n/a
13 TRCN0000058226 CCATTCCCTTATCCAGGCTTT pLKO.1 149 CDS 100% 4.050 2.025 Y GH2 n/a
14 TRCN0000160300 CAGAAGTATTCATTCCTGCAT pLKO.1 259 CDS 100% 2.640 1.320 Y CSH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000515.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491319 GCTCCGACTGCGGGTACAATAATC pLX_317 36.4% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_00634 pDONR223 100% 94.6% 93% None (many diffs) n/a
3 ccsbBroad304_00634 pLX_304 0% 94.6% 93% V5 (many diffs) n/a
4 TRCN0000478654 CGGCTAGGGTAGCTGAACACGAGT pLX_317 45.7% 94.6% 93% V5 (many diffs) n/a
5 ccsbBroadEn_06051 pDONR223 100% 92.7% 85.2% None (many diffs) n/a
6 ccsbBroad304_06051 pLX_304 0% 92.7% 85.2% V5 (many diffs) n/a
7 TRCN0000478974 TTCTAGGTATACTATTGCAGTGAG pLX_317 59.7% 92.7% 85.2% V5 (many diffs) n/a
8 ccsbBroadEn_00373 pDONR223 100% 92.6% 85.2% None (many diffs) n/a
9 ccsbBroad304_00373 pLX_304 0% 92.6% 85.2% V5 (many diffs) n/a
10 TRCN0000469020 CTTCGCCCGGCGTACGAATCACCC pLX_317 54.8% 92.6% 85.2% V5 (many diffs) n/a
11 ccsbBroadEn_00374 pDONR223 100% 92.4% 85.2% None (many diffs) n/a
12 ccsbBroad304_00374 pLX_304 0% 92.4% 85.2% V5 (many diffs) n/a
13 TRCN0000466754 AAACTGCTGAAATAACCAGGGAGG pLX_317 61.2% 92.4% 85.2% V5 (many diffs) n/a
14 ccsbBroadEn_15390 pDONR223 0% 92.4% 85.2% None (many diffs) n/a
15 ccsbBroad304_15390 pLX_304 0% 92.4% 85.2% V5 (many diffs) n/a
16 TRCN0000478638 GCAGCTCCTCTCTGGAGCAATCTC pLX_317 59.7% 92.4% 85.2% V5 (many diffs) n/a
17 ccsbBroadEn_00375 pDONR223 100% 54% 49.3% None (many diffs) n/a
18 ccsbBroad304_00375 pLX_304 0% 54% 49.3% V5 (many diffs) n/a
19 TRCN0000475609 ATAACCGATCTTCGAGTCTGGGAC pLX_317 61.6% 54% 49.3% V5 (many diffs) n/a
20 ccsbBroadEn_06052 pDONR223 100% 53.7% 49.3% None (many diffs) n/a
21 ccsbBroad304_06052 pLX_304 0% 53.7% 49.3% V5 (many diffs) n/a
22 TRCN0000466000 TAACGGGGAACGTTCGTTGATAAT pLX_317 85% 53.7% 49.3% V5 (many diffs) n/a
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