Construct: ORF TRCN0000466009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014642.1_s317c1
- Derived from:
- ccsbBroadEn_01928
- DNA Barcode:
- CCAACGCTTTGACCTTTGCAGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCK2 (8440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8440 | NCK2 | NCK adaptor protein 2 | NM_001004720.3 | 100% | 100% | |
| 2 | human | 8440 | NCK2 | NCK adaptor protein 2 | NM_003581.5 | 100% | 100% | |
| 3 | human | 8440 | NCK2 | NCK adaptor protein 2 | XM_006712797.3 | 100% | 100% | |
| 4 | human | 8440 | NCK2 | NCK adaptor protein 2 | XM_011511991.3 | 100% | 100% | |
| 5 | human | 8440 | NCK2 | NCK adaptor protein 2 | XM_017005103.1 | 100% | 100% | |
| 6 | human | 8440 | NCK2 | NCK adaptor protein 2 | XM_017005104.1 | 100% | 100% | |
| 7 | human | 8440 | NCK2 | NCK adaptor protein 2 | XM_017005105.1 | 100% | 100% | |
| 8 | human | 8440 | NCK2 | NCK adaptor protein 2 | NM_001004722.3 | 21.1% | 20% | (many diffs) |
| 9 | human | 8440 | NCK2 | NCK adaptor protein 2 | XM_011511992.2 | 21.1% | 20% | (many diffs) |
| 10 | mouse | 17974 | Nck2 | non-catalytic region of tyr... | NM_010879.3 | 88.5% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agaagaagtt attgtgatag ccaagtggga ctacaccgcc cagcaggacc 121 aggagctgga catcaagaag aacgagcggc tgtggttgct ggacgactcc aagacgtggt 181 ggcgggtgag gaacgcggcc aacaggacgg gctatgtacc gtccaactac gtggagcgga 241 agaacagcct gaagaagggc tccctcgtga agaacctgaa ggacacacta ggcctcggca 301 agacgcgcag gaagaccagc gcgcgggatg cgtcccccac gcccagcacg gacgccgagt 361 accccgccaa tggcagcggc gccgaccgca tctacgacct caacatcccg gccttcgtca 421 agttcgccta tgtggccgag cgggaggatg agttgtccct ggtgaagggg tcgcgcgtca 481 ccgtcatgga gaagtgcagc gacggttggt ggcggggcag ctacaacggg cagatcggct 541 ggttcccctc caactacgtc ttggaggagg tggacgaggc ggctgcggag tccccaagct 601 tcctgagcct gcgcaagggc gcctcgctga gcaatggcca gggctcccgc gtgctgcatg 661 tggtccagac gctgtacccc ttcagctcag tcaccgagga ggagctcaac ttcgagaagg 721 gggagaccat ggaggtgatt gagaagccgg agaacgaccc cgagtggtgg aaatgcaaaa 781 atgcccgggg ccaggtgggc ctcgtcccca aaaactacgt ggtggtcctc agtgacgggc 841 ctgccctgca ccctgcgcac gccccacaga taagctacac cgggcccTCG TCCAGCGGGC 901 GCTTCGCGGG CAGAGAGTGG TACTACGGGA ACGTGACGCG GCACCAGGCC GAGTGCGCCC 961 TCAACGAGCG GGGCGTGGAG GGCGACTTCC TCATTAGGGA CAGCGAGTCC TCGCCCAGCG 1021 ACTTCTCCGT GTCCCTTAAA GCGTCAGGGA AGAACAAACA CTTCAAGGTG CAGCTCGTGG 1081 ACAATGTCTA CTGCATTGGG CAGCGGCGCT TCCACACCAT GGACGAGCTG GTGGAACACT 1141 ACAAAAAGGC GCCCATCTTC ACCAGCGAGC ACGGGGAGAA GCTCTACCTC GTCAGGGCCC 1201 TGCAGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCAACGCTT TGACCTTTGC AGGAGACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt