Construct: ORF TRCN0000466033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017076.1_s317c1
- Derived from:
- ccsbBroadEn_01826
- DNA Barcode:
- TTATGATGGGTGCAAACGTCTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC30A4 (7782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7782 | SLC30A4 | solute carrier family 30 me... | NM_013309.6 | 100% | 100% | |
| 2 | human | 7782 | SLC30A4 | solute carrier family 30 me... | XM_011521997.3 | 100% | 100% | |
| 3 | human | 7782 | SLC30A4 | solute carrier family 30 me... | XM_017022560.2 | 100% | 100% | |
| 4 | human | 7782 | SLC30A4 | solute carrier family 30 me... | XM_017022561.1 | 45% | 43.1% | (many diffs) |
| 5 | human | 7782 | SLC30A4 | solute carrier family 30 me... | NM_001321036.2 | 43.1% | 41.9% | (many diffs) |
| 6 | mouse | 22785 | Slc30a4 | solute carrier family 30 (z... | NM_011774.3 | 88.8% | 91.8% | (many diffs) |
| 7 | mouse | 22785 | Slc30a4 | solute carrier family 30 (z... | NM_001290993.1 | 78.4% | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggctctggc gcgtggaagc gcctcaaatc tatgctaagg aaggatgatg 121 cgccgctgtt tttaaatgac accagcgcct ttgacttctc ggatgaggcg ggggacgagg 181 ggctttctcg gttcaacaaa cttcgagttg tggtggccga tgacggttcc gaagccccgg 241 aaaggcctgt taacggggcg cacccgaccc tccaggccga cgatgattcc ttactggacc 301 aagacttacc tttgaccaac agtcagctga gtttgaaggt ggactcctgt gacaactgca 361 gcaaacagag agagatactg aagcagagaa aggtgaaagc caggttgacc attgctgccg 421 ttctgtactt gcttttcatg attggagaac ttgtaggtgg atacattgca aatagcctag 481 caatcatgac agatgcactt catatgttaa ctgacctaag cgccatcata ctcaccctgc 541 ttgctttgtg gctatcatca aaatcaccaa ccaaaagatt cacctttgga tttcatcgct 601 tagaggtttt gtcagctatg attagtgtgc tgttggtgta tatacttatg ggattcctct 661 tatatgaagc tgtgcaaaga actatccata tgaactatga aataaatgga gatataatgc 721 tcatcaccgc agctgttgga gttgcagtta atgtaataat ggggtttctg ttgaaccagt 781 ctggtcaccg tcactcccat tcccactccc tgccttcaaa ttcccctacc agaggttctg 841 ggtgtgaacg taaccatggg caggatagcc tggcagtgag agctgcattt gtacatgctt 901 tgggagattt ggtacagagt gttgGTGTGC TAATAGCTGC ATACATCATA CGATTCAAGC 961 CAGAATACAA GATTGCTGAC CCCATCTGTA CATACGTATT TTCATTACTT GTGGCTTTTA 1021 CAACATTTCG AATCATATGG GATACAGTAG TTATAATACT AGAAGGTGTG CCAAGCCATT 1081 TGAATGTAGA CTATATCAAA GAAGCCTTGA TGAAAATAGA AGATGTATAT TCAGTCGAAG 1141 ATTTAAATAT CTGGTCTCTC ACTTCAGGAA AATCTACTGC CATAGTTCAC ATACAGCTAA 1201 TTCCTGGAAG TTCATCTAAA TGGGAGGAAG TACAGTCCAA AGCAAACCAT TTATTATTGA 1261 ACACATTTGG CATGTATAGA TGTACTATTC AGCTTCAGAG TTACAGGCAA GAAGTGGACA 1321 GAACTTGTGC AAATTGTCAG AGTTCTAGTC CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1501 ATGATGGGTG CAAACGTCTT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt