Construct: ORF TRCN0000466045

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002325.1_s317c1
Derived from:
ccsbBroadEn_08539
DNA Barcode:
TTGGCCCTCATGACACCCTCCTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASIC4 (55515)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466045
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55515 ASIC4 acid sensing ion channel su... NM_182847.2 99.8% 99.6% 1847G>T;1856T>C
2 human 55515 ASIC4 acid sensing ion channel su... NM_018674.5 97% 96.8% 1399_1455del;1904G>T;1913T>C
3 human 55515 ASIC4 acid sensing ion channel su... XM_017004439.1 45% 44.8% (many diffs)
4 mouse 241118 Asic4 acid-sensing (proton-gated)... NM_183022.3 71.1% 76.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2007
ORF length:
1941
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gagcggagcg gctggggctg cgcggcgtgg cggagcagcg ctcgctccct
121cgctcactcg ctcgctcgca gggacacacg caggggctga cagctgtgct ggtgctgata
181agggaagcca caaggagacg atcgaggaga gagacaagcg gcagcagagg cagcagcggc
241agaggcagca ccagggctgc ggagctgctg ggagtgggag tgactccccc acctcgggcc
301cccaccctgt ccctgtcctc ttcccgcttg ccctgagttt agaagagcag ccgctgccac
361cactgccact cgggagggca ccagggctgc tggctaggga gggacagggc agggaggctc
421tggccagtcc cagcagccgg ggacagatgc cgatcgagat tgtgtgcaaa atcaaatttg
481ctgaggagga tgcgaaaccc aaggagaagg aggcagggga tgagcagagc ctcctcgggg
541ctgttgcccc tggagcagcc ccccgagacc tggccacctt tgccagcacc agcaccctgc
601atggactggg ccgggcctgt ggcccaggcc cccacggact gcgcagaacc ctgtgggcac
661tggccctact cacctcgctg gctgccttcc tgtaccaggc ggctggcctg gcccggggct
721acctgacccg gcctcacctg gtggcaatgg accccgctgc cccagcccca gtggcgggct
781tcccggctgt caccctctgc aatatcaacc gcttccggca ttcggcactc agcgatgccg
841acatcttcca cctggccaat ctgacagggc tgccccccaa agaccgggat gggcaccgtg
901cggctggcct gcgctaccca gagcctgaca tggtagacat cctcaaccgc actggccacc
961agctcgccga catgcttaag agctgcaact tcagtgggca tcactgctcc gccagcaact
1021tctctgtggt ctatactcgc tatgggaagt gttacacctt caacgcggac ccgcggagct
1081cgctgcccag ccgggcaggg ggcatgggca gtggcctgga gatcatgctg gacatccagc
1141aggaggagta cctgcccatc tggagggaga caaatgagac gtcgtttgag gcaggtattc
1201gggtgcagat ccacagccag gaggagccgc cctacatcca ccagctgggg ttcggggtgt
1261ccccaggctt ccagaccttt gtgtcctgcc aggaacagcg gctgacctac ctgccccagc
1321cctggggcaa ctgccgcgca gagagtgagc tcagggagcc tgagcttcag ggctactcgg
1381cctacagtgt gtctgcctgc cggctgcgct gtgaaaagga ggccgtgctt cagcgctgcc
1441actgccggat ggtgcacatg ccagactccc tgggtggggg ccctgagggc ccgtgcttct
1501gccccacccc ctgcaacctg acacgctatg ggaaagagat ctccatggtc aggatcccca
1561acaggggctc agcccggtac ctggcgagga agtacaaccg caacgagacc tacatacggg
1621agaacttcct ggtcctagat gtcttctttg aggccctgac ctctgaagcc atggagcagc
1681gagcagccTA TGGCCTGTCA GCCCTGCTGG GAGACCTCGG GGGACAGATG GGCCTGTTCA
1741TTGGGGCCAG CATCCTCACG TTGCTGGAGA TCCTCGACTA CATCTATGAG GTGTCCTGGG
1801ATCGACTGAA GCGGGTATGG AGGCGTCCCA AGACCCCCCT GCGGACCTCC ACTGGGGGCA
1861TCTCCACTTT GGGGCTTCAG GAGCTGAAGG AACAGAGTCC CTGCCCGAGC CTGGGCCGAG
1921CGGAGGGTGG GGGGGTCAGC AGTCTGCTCC CCAATCACCA CCACCCCCAC GGTCCCCCAG
1981GAGGTCTCTT TGAAGATTTT GCTTGCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG
2041GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2101AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTGGCCCT
2161CATGACACCC TCCTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2221aagatt

Download FASTA (ORF) (Full)