Construct: ORF TRCN0000466073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006721.1_s317c1
- Derived from:
- ccsbBroadEn_12419
- DNA Barcode:
- AATTCCCCCCCCAGACCGACGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SINHCAF (58516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | NM_001135811.1 | 33% | 33% | 1_444del |
| 2 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | NM_001135812.2 | 33% | 33% | 1_444del |
| 3 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | NM_021238.2 | 33% | 33% | 1_444del |
| 4 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019758.2 | 33% | 33% | 1_444del |
| 5 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019759.2 | 33% | 33% | 1_444del |
| 6 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019760.1 | 33% | 33% | 1_444del |
| 7 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019761.1 | 33% | 33% | 1_444del |
| 8 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_017019762.2 | 33% | 33% | 1_444del |
| 9 | human | 58516 | SINHCAF | SIN3-HDAC complex associate... | XM_011520803.2 | 22.2% | 22.2% | 1_765del |
| 10 | mouse | 56306 | Fam60a | family with sequence simila... | NM_019643.3 | 30.3% | 31.6% | (many diffs) |
| 11 | mouse | 56306 | Fam60a | family with sequence simila... | XM_006507074.3 | 30.3% | 31.6% | (many diffs) |
| 12 | mouse | 56306 | Fam60a | family with sequence simila... | XM_006507075.2 | 30.3% | 31.6% | (many diffs) |
| 13 | mouse | 56306 | Fam60a | family with sequence simila... | XM_006507073.3 | 19% | 19.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 361
- ORF length:
- 219
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGGCTTCT GGTTCTAACA GAACACCAGT TTTTTCCTTT 181 TTAGATCTCA CTTACTGGAA AAGACAGAAG ATATGTTGTG GGATCATCTA TAAAGGCCGT 241 TTTGGGGAAG TCCTCATTGA CACACATCTC TTCAAGCCTT GCTGCAGCAA TAAGAAAGCA 301 GCTGCTGAGA AGCCAGAGGA GCAGGGGCCA GAGCCTCTGC CCATCTCCAC TCAGGAGTGG 361 TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 421 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 481 CTTTATATAT CTTGTGGAAA GGACGAAATT CCCCCCCCAG ACCGACGGGC ACGCGTTAAG 541 TCgacaatca acctctggat tacaaaattt gtgaaagatt