Construct: ORF TRCN0000466093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014210.1_s317c1
- Derived from:
- ccsbBroadEn_03462
- DNA Barcode:
- ACATGTGCGCCCTATACTCCACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL28 (54813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54813 | KLHL28 | kelch like family member 28 | NM_017658.5 | 100% | 100% | |
| 2 | human | 54813 | KLHL28 | kelch like family member 28 | XM_005267770.4 | 100% | 100% | |
| 3 | human | 54813 | KLHL28 | kelch like family member 28 | XM_011536847.3 | 100% | 100% | |
| 4 | human | 54813 | KLHL28 | kelch like family member 28 | NM_001308112.2 | 97.6% | 97.6% | 1_42del |
| 5 | human | 54813 | KLHL28 | kelch like family member 28 | XM_024449635.1 | 78.9% | 77.9% | (many diffs) |
| 6 | human | 54813 | KLHL28 | kelch like family member 28 | XM_011536849.2 | 77% | 76% | (many diffs) |
| 7 | human | 54813 | KLHL28 | kelch like family member 28 | XM_017021378.1 | 53.7% | 52.8% | (many diffs) |
| 8 | human | 54813 | KLHL28 | kelch like family member 28 | XM_006720174.3 | 52.4% | 51.6% | (many diffs) |
| 9 | mouse | 66689 | Klhl28 | kelch-like 28 | NM_025707.3 | 91.1% | 97% | (many diffs) |
| 10 | mouse | 66689 | Klhl28 | kelch-like 28 | XM_006516156.3 | 91.1% | 97% | (many diffs) |
| 11 | mouse | 66689 | Klhl28 | kelch-like 28 | XM_006516157.3 | 86.5% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1782
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaccacaca tccccgacct acatgcttgc taacttaacc cacttgcatt 121 ctgaacaact tctgcagggc ttgaatcttc ttcgccaaca tcacgaactc tgtgacatca 181 ttcttcgagt aggtgatgtt aaaattcatg ctcacaaagt ggtacttgcc agcgtcagcc 241 cgtatttcaa agctatgttc actggaaacc tttctgaaaa agagaacagt gaggttgagt 301 ttcaatgcat tgatgaaact gctctccagg ccattgtgga gtatgcctat acagggactg 361 tttttatttc tcaggacaca gttgaatctc tcctgccagc agcaaaccta ctccagataa 421 aacttgtcct gaaagaatgt tgtgcatttc ttgaaagcca acttgatcct ggtaattgta 481 ttggaatttc tcgttttgca gaaacatatg gttgccgtga cctttatttg gcagccacta 541 aatacatatg ccagaatttt gaagctgttt gccagactga agagtttttt gagcttacac 601 atgctgactt ggatgaaatt gtttccaatg actgtttgaa tgtagctacc gaagagactg 661 ttttttatgc attagagtct tggatcaagt atgatgtaca agaacgccag aaatacttag 721 cacagttact aaacagtgta cgattaccat tgttgagtgt taagtttctc actagactat 781 atgaagcaaa tcatcttatt cgtgatgatc gcacttgtaa acatcttttg aatgaagccc 841 taaagtacca ctttatgcct gaacatagac tctctcatca gacagtcttg atgacacgac 901 ctcgctgtgc tcccaaagta ctttgtgcag taggagggaa atctggactc tttgcctgtt 961 tggatagtgt ggagatgtac tttcctcaga atgactcttg gattggtttg gcacccctaa 1021 acattcctcg ctatgaattt ggaatatgcg ttttagacca aaaagtatat gttataggtg 1081 gtattgcaac taatgtgcgt cctggcgtca ctatcagaaa acatgaaaat tcagtggaat 1141 gctggaatcc tgatacaaat acttggactt ctctagagag aatgaatgaa agccgaagta 1201 ctcttggagt agtagtactt gcaggagaac tttatgcctt aggtggttat gatggacaat 1261 cttatttaca atctgtagag aagtacattc ccaaaataag aaaatggcaa cctgtggcac 1321 caatgacgac aacaagaagt tgttttgctg cagcggtatt ggatggaatg atatatgcca 1381 ttggtgggta tggTCCTGCC CACATGAACA GTGTGGAGCG TTATGATCCA AGTAAGGACT 1441 CCTGGGAGAT GGTTGCATCC ATGGCAGATA AAAGGATTCA CTTTGGCGTG GGTGTCATGC 1501 TAGGCTTTAT TTTTGTGGTG GGTGGACATA ATGGAGTCTC ACATTTGTCC AGCATTGAAA 1561 GATACGATCC TCATCAAAAT CAGTGGACTG TGTGTAGACC AATGAAAGAA CCTAGAACAG 1621 GAGTTGGTGC TGCTGTAATC GATAACTACC TTTATGTCGT CGGTGGTCAC TCAGGGTCTT 1681 CCTATCTGAA TACAGTGCAG AAATATGATC CTATCTCAGA TACGTGGCTG GATTCAGCTG 1741 GCATGATATA CTGTCGCTGC AACTTTGGGT TAACTGCACT TTTGCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGAACA TGTGCGCCCT ATACTCCACA GACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t