Transcript: Mouse XM_006516156.3

PREDICTED: Mus musculus kelch-like 28 (Klhl28), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Klhl28 (66689)
Length:
3745
CDS:
66..1781

Additional Resources:

NCBI RefSeq record:
XM_006516156.3
NBCI Gene record:
Klhl28 (66689)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006516156.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000246554 CATGCTGATTTGGACGAAATT pLKO_005 597 CDS 100% 13.200 18.480 N Klhl28 n/a
2 TRCN0000257527 GTACTTTGAACTGACTAATAG pLKO_005 1910 3UTR 100% 13.200 18.480 N Klhl28 n/a
3 TRCN0000246555 GAGCGTTACGATCCGAGTAAG pLKO_005 1413 CDS 100% 10.800 15.120 N Klhl28 n/a
4 TRCN0000179309 GCTTGCTAACTTAACCCACTT pLKO.1 92 CDS 100% 4.050 5.670 N Klhl28 n/a
5 TRCN0000183558 GCTTCTGATAGTGGATGTAAA pLKO.1 2522 3UTR 100% 13.200 10.560 N Klhl28 n/a
6 TRCN0000246556 CCAACTTGACCCTGGTAATTG pLKO_005 455 CDS 100% 13.200 9.240 N Klhl28 n/a
7 TRCN0000246553 GGATTCAGCAGGCATGATATA pLKO_005 1727 CDS 100% 13.200 9.240 N Klhl28 n/a
8 TRCN0000179421 CTATGTTCACTGGCAACCTTT pLKO.1 250 CDS 100% 4.950 3.465 N Klhl28 n/a
9 TRCN0000141463 CATGATATACTGTCGCTGCAA pLKO.1 1739 CDS 100% 2.640 1.848 N KLHL28 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006516156.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03462 pDONR223 100% 91.1% 97% None (many diffs) n/a
2 ccsbBroad304_03462 pLX_304 0% 91.1% 97% V5 (many diffs) n/a
3 TRCN0000466093 ACATGTGCGCCCTATACTCCACAG pLX_317 22.9% 91.1% 97% V5 (many diffs) n/a
4 ccsbBroadEn_15871 pDONR223 0% 80.8% 85.2% None (many diffs) n/a
5 ccsbBroad304_15871 pLX_304 0% 80.8% 85.2% V5 (many diffs) n/a
Download CSV