Transcript: Human XM_011536849.2

PREDICTED: Homo sapiens kelch like family member 28 (KLHL28), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KLHL28 (54813)
Length:
6904
CDS:
652..2061

Additional Resources:

NCBI RefSeq record:
XM_011536849.2
NBCI Gene record:
KLHL28 (54813)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011536849.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000145580 CAAATGTGAACTCTCGGAAAT pLKO.1 2201 3UTR 100% 10.800 15.120 N KLHL28 n/a
2 TRCN0000144111 CCTCGCTATGAATTTGGAATA pLKO.1 1651 CDS 100% 10.800 15.120 N KLHL28 n/a
3 TRCN0000141463 CATGATATACTGTCGCTGCAA pLKO.1 2158 3UTR 100% 2.640 3.696 N KLHL28 n/a
4 TRCN0000140525 GTACTGCATGAACATCCGGAT pLKO.1 2240 3UTR 100% 2.160 3.024 N KLHL28 n/a
5 TRCN0000142103 GCTATGTTCACTGGAAACCTT pLKO.1 877 CDS 100% 3.000 2.400 N KLHL28 n/a
6 TRCN0000144659 GCTGGAATCCTGATACAAATA pLKO.1 1766 CDS 100% 13.200 9.240 N KLHL28 n/a
7 TRCN0000419995 TGGTGCTGCTGTAATCGATAA pLKO_005 2041 CDS 100% 10.800 7.560 N KLHL28 n/a
8 TRCN0000141219 CAGCCCGTATTTCAAAGCTAT pLKO.1 861 CDS 100% 4.950 3.465 N KLHL28 n/a
9 TRCN0000179309 GCTTGCTAACTTAACCCACTT pLKO.1 720 CDS 100% 4.050 2.835 N Klhl28 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 4440 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4441 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011536849.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15871 pDONR223 0% 86.3% 85.1% None (many diffs) n/a
2 ccsbBroad304_15871 pLX_304 0% 86.3% 85.1% V5 (many diffs) n/a
3 ccsbBroadEn_03462 pDONR223 100% 77% 76% None (many diffs) n/a
4 ccsbBroad304_03462 pLX_304 0% 77% 76% V5 (many diffs) n/a
5 TRCN0000466093 ACATGTGCGCCCTATACTCCACAG pLX_317 22.9% 77% 76% V5 (many diffs) n/a
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