Construct: ORF TRCN0000466127

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012385.2_s317c1
Derived from:
ccsbBroadEn_06782
DNA Barcode:
ACTCAGTGCCGCACGCAGTATTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPS2 (5634)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466127
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5634 PRPS2 phosphoribosyl pyrophosphat... NM_002765.4 99.8% 100% 45G>A
2 human 5634 PRPS2 phosphoribosyl pyrophosphat... NM_001039091.2 98.9% 99% 45G>A;305_313delAGGTAGGAG
3 human 5631 PRPS1 phosphoribosyl pyrophosphat... NM_002764.4 80% 95.2% (many diffs)
4 human 221823 PRPS1L1 phosphoribosyl pyrophosphat... NM_175886.3 79.3% 90.8% (many diffs)
5 human 5631 PRPS1 phosphoribosyl pyrophosphat... NM_001204402.1 28.9% 34.9% (many diffs)
6 mouse 110639 Prps2 phosphoribosyl pyrophosphat... NM_026662.5 88.9% 98.7% (many diffs)
7 mouse 110639 Prps2 phosphoribosyl pyrophosphat... NM_001313758.1 88.1% 97.8% (many diffs)
8 mouse 110639 Prps2 phosphoribosyl pyrophosphat... XM_017318352.1 36.8% 23.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1020
ORF length:
954
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc caacatcgtg ctgttcagcg gcagctcgca tcaggaccta tcccagcgcg
121tggccgaccg cctgggcctg gagctgggca aggtggtcac gaagaagttc agcaaccagg
181agaccagcgt ggagattggt gaaagcgtga gaggggaaga tgtctacatc atccagagcg
241gctgcgggga aattaacgac aacctgatgg aactcctcat catgatcaat gcctgcaaga
301ttgcgtcatc atccagagta actgccgtga tcccgtgttt cccatacgcc cgacaagata
361aaaaggacaa gagtcgtgcc ccaatttctg caaaacttgt ggccaatatg ctgtcggtgg
421ctggggcgga tcacatcatc accatggacc tgcatgcttc tcagatacag ggattctttg
481atattcctgt ggataatttg tatgcggagc ccgcagtcct gcagtggatt cgggaaaaca
541ttgccgagtg gaagaactgt atcattgttt cacctgacgc agggggagcc aaaagggtta
601catcaattgc agacaggttg aatgtggaat ttgctttgaT CCACAAAGAG AGGAAGAAGG
661CGAATGAAGT GGACCGGATG GTCCTGGTGG GCGACGTGAA GGACCGTGTG GCCATCCTCG
721TGGATGACAT GGCTGACACT TGCGGCACCA TCTGCCATGC TGCGGACAAG CTGCTGTCAG
781CTGGAGCCAC CAAAGTGTAT GCTATCCTTA CCCATGGGAT CTTCTCTGGA CCAGCTATTT
841CCAGAATAAA TAATGCCGCC TTTGAGGCTG TTGTCGTCAC AAACACAATT CCGCAAGAGG
901ACAAAATGAA ACACTGCACC AAGATTCAGG TCATTGACAT TTCCATGATC TTGGCCGAAG
961CAATCCGAAG GACACACAAT GGGGAATCCG TGTCCTACCT GTTCAGCCAT GTCCCGCTAT
1021ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1081GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1141TTTATATATC TTGTGGAAAG GACGAACTCA GTGCCGCACG CAGTATTGTA CGCGTTAAGT
1201Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)