Construct: ORF TRCN0000466127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012385.2_s317c1
- Derived from:
- ccsbBroadEn_06782
- DNA Barcode:
- ACTCAGTGCCGCACGCAGTATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPS2 (5634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5634 | PRPS2 | phosphoribosyl pyrophosphat... | NM_002765.4 | 99.8% | 100% | 45G>A |
| 2 | human | 5634 | PRPS2 | phosphoribosyl pyrophosphat... | NM_001039091.2 | 98.9% | 99% | 45G>A;305_313delAGGTAGGAG |
| 3 | human | 5631 | PRPS1 | phosphoribosyl pyrophosphat... | NM_002764.4 | 80% | 95.2% | (many diffs) |
| 4 | human | 221823 | PRPS1L1 | phosphoribosyl pyrophosphat... | NM_175886.3 | 79.3% | 90.8% | (many diffs) |
| 5 | human | 5631 | PRPS1 | phosphoribosyl pyrophosphat... | NM_001204402.1 | 28.9% | 34.9% | (many diffs) |
| 6 | mouse | 110639 | Prps2 | phosphoribosyl pyrophosphat... | NM_026662.5 | 88.9% | 98.7% | (many diffs) |
| 7 | mouse | 110639 | Prps2 | phosphoribosyl pyrophosphat... | NM_001313758.1 | 88.1% | 97.8% | (many diffs) |
| 8 | mouse | 110639 | Prps2 | phosphoribosyl pyrophosphat... | XM_017318352.1 | 36.8% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caacatcgtg ctgttcagcg gcagctcgca tcaggaccta tcccagcgcg 121 tggccgaccg cctgggcctg gagctgggca aggtggtcac gaagaagttc agcaaccagg 181 agaccagcgt ggagattggt gaaagcgtga gaggggaaga tgtctacatc atccagagcg 241 gctgcgggga aattaacgac aacctgatgg aactcctcat catgatcaat gcctgcaaga 301 ttgcgtcatc atccagagta actgccgtga tcccgtgttt cccatacgcc cgacaagata 361 aaaaggacaa gagtcgtgcc ccaatttctg caaaacttgt ggccaatatg ctgtcggtgg 421 ctggggcgga tcacatcatc accatggacc tgcatgcttc tcagatacag ggattctttg 481 atattcctgt ggataatttg tatgcggagc ccgcagtcct gcagtggatt cgggaaaaca 541 ttgccgagtg gaagaactgt atcattgttt cacctgacgc agggggagcc aaaagggtta 601 catcaattgc agacaggttg aatgtggaat ttgctttgaT CCACAAAGAG AGGAAGAAGG 661 CGAATGAAGT GGACCGGATG GTCCTGGTGG GCGACGTGAA GGACCGTGTG GCCATCCTCG 721 TGGATGACAT GGCTGACACT TGCGGCACCA TCTGCCATGC TGCGGACAAG CTGCTGTCAG 781 CTGGAGCCAC CAAAGTGTAT GCTATCCTTA CCCATGGGAT CTTCTCTGGA CCAGCTATTT 841 CCAGAATAAA TAATGCCGCC TTTGAGGCTG TTGTCGTCAC AAACACAATT CCGCAAGAGG 901 ACAAAATGAA ACACTGCACC AAGATTCAGG TCATTGACAT TTCCATGATC TTGGCCGAAG 961 CAATCCGAAG GACACACAAT GGGGAATCCG TGTCCTACCT GTTCAGCCAT GTCCCGCTAT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAACTCA GTGCCGCACG CAGTATTGTA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt