Transcript: Mouse NM_001313758.1

Mus musculus phosphoribosyl pyrophosphate synthetase 2 (Prps2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Prps2 (110639)
Length:
3719
CDS:
159..1124

Additional Resources:

NCBI RefSeq record:
NM_001313758.1
NBCI Gene record:
Prps2 (110639)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001313758.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276708 GTGGTTATTTGGTCGTTAATT pLKO_005 1332 3UTR 100% 15.000 21.000 N Prps2 n/a
2 TRCN0000276707 CAGTGTCGAGATCGGTGAAAG pLKO_005 278 CDS 100% 10.800 15.120 N Prps2 n/a
3 TRCN0000025540 CGTGCAAGATTGCGTCCTCAT pLKO.1 385 CDS 100% 4.050 5.670 N Prps2 n/a
4 TRCN0000285618 CGTGCAAGATTGCGTCCTCAT pLKO_005 385 CDS 100% 4.050 5.670 N Prps2 n/a
5 TRCN0000025541 CCAGCTATTTCCAGAATAAAT pLKO.1 933 CDS 100% 15.000 10.500 N Prps2 n/a
6 TRCN0000276646 CCAGCTATTTCCAGAATAAAT pLKO_005 933 CDS 100% 15.000 10.500 N Prps2 n/a
7 TRCN0000025539 GCCACCAAAGTTTATGCTATT pLKO.1 888 CDS 100% 10.800 7.560 N Prps2 n/a
8 TRCN0000025543 GCAGACAGACTGAATGTGGAA pLKO.1 711 CDS 100% 2.640 1.848 N Prps2 n/a
9 TRCN0000276647 GCAGACAGACTGAATGTGGAA pLKO_005 711 CDS 100% 2.640 1.848 N Prps2 n/a
10 TRCN0000025542 GTGTCCTATCTGTTCAGCCAT pLKO.1 1092 CDS 100% 2.640 1.848 N Prps2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001313758.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01298 pDONR223 100% 89% 98.7% None (many diffs) n/a
2 ccsbBroad304_01298 pLX_304 0% 89% 98.7% V5 (many diffs) n/a
3 TRCN0000474643 CACTTTTGCAGGAATGATATTATT pLX_317 35.3% 89% 98.7% V5 (many diffs) n/a
4 ccsbBroadEn_01299 pDONR223 100% 88.2% 97.8% None (many diffs) n/a
5 ccsbBroad304_01299 pLX_304 0% 88.2% 97.8% V5 (many diffs) n/a
6 TRCN0000475829 AATCCTTTTAGAGGCTTGGACTGC pLX_317 32% 88.2% 97.8% V5 (many diffs) n/a
7 ccsbBroadEn_06782 pDONR223 100% 88.1% 97.8% None (many diffs) n/a
8 TRCN0000466127 ACTCAGTGCCGCACGCAGTATTGT pLX_317 40.1% 88.1% 97.8% V5 (many diffs) n/a
9 ccsbBroadEn_14816 pDONR223 0% 88.1% 97.8% None (many diffs) n/a
10 ccsbBroad304_14816 pLX_304 0% 88.1% 97.8% V5 (many diffs) n/a
11 TRCN0000471920 CGTGGCCTACGACTTCCGGATCCG pLX_317 35.5% 88.1% 97.8% V5 (many diffs) n/a
12 TRCN0000492233 GTTTTTGGCATTCGTGCCCAATAT pLX_317 43.2% 88.1% 97.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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