Construct: ORF TRCN0000466140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015563.1_s317c1
- Derived from:
- ccsbBroadEn_00954
- DNA Barcode:
- TAAATGCTCCGCACTTCGTAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LYN (4067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | NM_002350.4 | 100% | 100% | |
| 2 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | NM_001111097.3 | 95.8% | 95.8% | 66_67ins63 |
| 3 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | XM_017013416.1 | 95.8% | 95.8% | 66_67ins63 |
| 4 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | XM_011517529.3 | 71.3% | 66.9% | (many diffs) |
| 5 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | XM_017013415.2 | 44.8% | 40.4% | (many diffs) |
| 6 | mouse | 17096 | Lyn | LYN proto-oncogene, Src fam... | NM_001111096.1 | 88.9% | 96% | (many diffs) |
| 7 | mouse | 17096 | Lyn | LYN proto-oncogene, Src fam... | NM_010747.2 | 84.9% | 92.1% | (many diffs) |
| 8 | mouse | 17096 | Lyn | LYN proto-oncogene, Src fam... | XM_011249939.2 | 73.1% | 80.2% | (many diffs) |
| 9 | mouse | 666513 | Gm11787 | predicted gene 11787 | XM_017320471.2 | 51.4% | 53.7% | (many diffs) |
| 10 | mouse | 108168924 | LOC108168924 | uncharacterized LOC108168924 | XR_001784222.1 | 9.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1605
- ORF length:
- 1536
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggatgtata aaatcaaaag ggaaagacag cttgagtgac gatggagtag 121 atttgaagac tcaaccagta cgtaatactg aaagaactat ttatgtgaga gatccaacgt 181 ccaataaaca gcaaaggcca gttccagaat ctcagctttt acctggacag aggtttcaaa 241 ctaaagatcc agaggaacaa ggagacattg tggtagcctt gtacccctat gatggcatcc 301 acccggacga cttgtctttc aagaaaggag agaagatgaa agtcctggag gagcatggag 361 aatggtggaa agcaaagtcc cttttaacaa aaaaagaagg cttcatcccc agcaactatg 421 tggccaaact caacacctta gaaacagaag agtggttttt caaggatata accaggaagg 481 acgcagaaag gcagcttttg gcaccaggaa atagcgctgg agctttcctt attagagaaa 541 gtgaaacatt aaaaggaagc ttctctctgt ctgtcagaga ctttgaccct gtgcatggtg 601 atgttattaa gcactacaaa attagaagtc tggataatgg gggctattac atctctccac 661 gaatcacttt tccctgtatc agcgacatga ttaaacatta ccaaaagcag gcagatggct 721 tgtgcagaag attggagaag gcttgtatta gtcccaagcc acagaagcca tgggataaag 781 atgcctggga gatcccccgg gagtccatca agttggtgaa aaggcttggc gctgggcagt 841 ttggggaagt ctggatgggt tactataaca acagtaccaa ggtggctgtg aaaaccctga 901 agccaggaac tatgtctgtg caagccttcc tggaagaagc caacctcatg aagaccctgc 961 agcatgacaa gctcgtgagg ctctacgctg tggtcaccag ggaggagccc atttacatca 1021 tcaccgagta catggccaag ggcagtttgc tggatttcct gaagagcgat gaaggtggca 1081 aagtgctgct tccaaagctc attgactttt ctgctcagat tgcagaggga atggcataca 1141 tcgagcggaa gaactacatt caccgggacc tgcgagcagc taatgttctg gtctccgagt 1201 cactcatgtg caaaattgca gattttGGCC TTGCTAGAGT AATTGAAGAT AATGAGTACA 1261 CAGCAAGGGA AGGTGCTAAG TTCCCTATTA AGTGGACGGC TCCAGAAGCA ATCAACTTTG 1321 GATGTTTCAC TATTAAGTCT GATGTGTGGT CCTTTGGAAT CCTCCTATAC GAAATTGTCA 1381 CCTATGGGAA AATTCCCTAC CCAGGGAGAA CTAATGCCGA CGTGATGACC GCCCTGTCCC 1441 AGGGCTACAG GATGCCCCGT GTGGAGAACT GCCCAGATGA GCTCTATGAC ATTATGAAAA 1501 TGTGCTGGAA AGAAAAGGCA GAAGAGAGAC CAACGTTTGA CTACTTACAG AGCGTCCTGG 1561 ATGATTTCTA CACAGCCACG GAAGGGCAAT ACCAGCAGCA GCCTTTGCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA TAAATGCTCC GCACTTCGTA ATCCACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt