Construct: ORF TRCN0000466141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005342.1_s317c1
- Derived from:
- ccsbBroadEn_08548
- DNA Barcode:
- GTACTTAGTCCGCCGTTTAACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRNA9 (55584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466141
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55584 | CHRNA9 | cholinergic receptor nicoti... | NM_017581.4 | 99.9% | 99.7% | 1325A>G |
| 2 | mouse | 231252 | Chrna9 | cholinergic receptor, nicot... | NM_001081104.1 | 85.4% | 90.8% | (many diffs) |
| 3 | mouse | 231252 | Chrna9 | cholinergic receptor, nicot... | XM_006503945.2 | 81.9% | 87% | (many diffs) |
| 4 | mouse | 231252 | Chrna9 | cholinergic receptor, nicot... | XM_006503946.3 | 81.5% | 88.5% | (many diffs) |
| 5 | mouse | 231252 | Chrna9 | cholinergic receptor, nicot... | XM_006503947.2 | 73.7% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1506
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaactggtcc cattcctgca tctccttttg ctggatctac tttgctgctt 121 ccagactgag agctgcagag acggcagatg gaaaatatgc tcagaagttg tttaatgacc 181 tttttgaaga ttattctaat gctcttcgtc cagtggaaga tacagataaa gtcctgaatg 241 tgaccctgca gattacgctc tctcagatta aggatatgga tgaaagaaac caaattctga 301 ctgcttattt gtggatccgc caaatctggc acgatgccta tctcacgtgg gaccgagatc 361 agtacgatgg cctagactcc atcaggatcc ccagtgacct cgtgtggagg ccagacatcg 421 tcttatataa caaggctgat gatgaatctt cagagcctgt gaacaccaat gtggtcctgc 481 ggtatgatgg gctgatcacc tgggatgcac cggccatcac caaaagctcc tgtgtggtgg 541 atgtcaccta cttccctttt gacaaccagc agtgcaacct gacttttggt tcctggacct 601 acaatggcaa tcaggtggac atattcaacg ccttggacag cggagatctc tctgacttca 661 ttgaagatgt ggaatgggag gtccatggca tgcccgctgt gaagaatgtg atctcctatg 721 gctgctgctc tgagccttac ccggatgtca cattcaccct ccttctgaag aggaggtcct 781 cgttctatat cgtcaacctc ctcatcccat gcgtcctcat atcttttctg gctcctctga 841 gtttttatct cccagcagcc tccggagaaa aggtctccct gggagtgacc atcctgttgg 901 ccatgactgt atttcagcta atggtggcag aaatcatgcc ggcctcagaa aatgtgcccc 961 tgataggtaa atactacata gccacgatgg ccctgatcac agcctccact gcgttgacca 1021 tcatggtgat gaatatccac ttctgtgggg ccgaggcccg gccggtgcca cactgggcca 1081 gggtggtcat cctgaaatac atgtccaggg tcttgtttgt ctatgatgtg ggtgaaagct 1141 gccTCAGCCC GCACCACAGT AGAGAGCGGG ACCACCTCAC GAAAGTTTAT AGCAAACTCC 1201 CAGAGTCTAA CCTGAAAGCA GCCAGGAACA AAGACCTTTC CAGAAAGAAG GACATGAACA 1261 AACGCTTAAA GAACGACCTG GGCTGCCAGG GTAAGAACCC TCAGGAGGCC GAGAGTTACT 1321 GTGCACAGTA CAAAGTGCTG ACGAGGAATA TTGAGTACAT CGCCAAGTGC CTCAAAGACC 1381 ACAAGGCCAC CAGTTCCAAG GGGAGTGAAT GGAAGAAGGT GGCGAAAGTC ATAGACCGAT 1441 TCTTCATGTG GATTTTTTTC ATTATGGTGT TTGTGATGAC TATTTTGATC ATAGCAAGAG 1501 CGGATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTACTTAGT CCGCCGTTTA ACTCAACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt