Construct: ORF TRCN0000466141

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005342.1_s317c1
Derived from:
ccsbBroadEn_08548
DNA Barcode:
GTACTTAGTCCGCCGTTTAACTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRNA9 (55584)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466141
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55584 CHRNA9 cholinergic receptor nicoti... NM_017581.4 99.9% 99.7% 1325A>G
2 mouse 231252 Chrna9 cholinergic receptor, nicot... NM_001081104.1 85.4% 90.8% (many diffs)
3 mouse 231252 Chrna9 cholinergic receptor, nicot... XM_006503945.2 81.9% 87% (many diffs)
4 mouse 231252 Chrna9 cholinergic receptor, nicot... XM_006503946.3 81.5% 88.5% (many diffs)
5 mouse 231252 Chrna9 cholinergic receptor, nicot... XM_006503947.2 73.7% 78.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1506
ORF length:
1437
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaactggtcc cattcctgca tctccttttg ctggatctac tttgctgctt
121ccagactgag agctgcagag acggcagatg gaaaatatgc tcagaagttg tttaatgacc
181tttttgaaga ttattctaat gctcttcgtc cagtggaaga tacagataaa gtcctgaatg
241tgaccctgca gattacgctc tctcagatta aggatatgga tgaaagaaac caaattctga
301ctgcttattt gtggatccgc caaatctggc acgatgccta tctcacgtgg gaccgagatc
361agtacgatgg cctagactcc atcaggatcc ccagtgacct cgtgtggagg ccagacatcg
421tcttatataa caaggctgat gatgaatctt cagagcctgt gaacaccaat gtggtcctgc
481ggtatgatgg gctgatcacc tgggatgcac cggccatcac caaaagctcc tgtgtggtgg
541atgtcaccta cttccctttt gacaaccagc agtgcaacct gacttttggt tcctggacct
601acaatggcaa tcaggtggac atattcaacg ccttggacag cggagatctc tctgacttca
661ttgaagatgt ggaatgggag gtccatggca tgcccgctgt gaagaatgtg atctcctatg
721gctgctgctc tgagccttac ccggatgtca cattcaccct ccttctgaag aggaggtcct
781cgttctatat cgtcaacctc ctcatcccat gcgtcctcat atcttttctg gctcctctga
841gtttttatct cccagcagcc tccggagaaa aggtctccct gggagtgacc atcctgttgg
901ccatgactgt atttcagcta atggtggcag aaatcatgcc ggcctcagaa aatgtgcccc
961tgataggtaa atactacata gccacgatgg ccctgatcac agcctccact gcgttgacca
1021tcatggtgat gaatatccac ttctgtgggg ccgaggcccg gccggtgcca cactgggcca
1081gggtggtcat cctgaaatac atgtccaggg tcttgtttgt ctatgatgtg ggtgaaagct
1141gccTCAGCCC GCACCACAGT AGAGAGCGGG ACCACCTCAC GAAAGTTTAT AGCAAACTCC
1201CAGAGTCTAA CCTGAAAGCA GCCAGGAACA AAGACCTTTC CAGAAAGAAG GACATGAACA
1261AACGCTTAAA GAACGACCTG GGCTGCCAGG GTAAGAACCC TCAGGAGGCC GAGAGTTACT
1321GTGCACAGTA CAAAGTGCTG ACGAGGAATA TTGAGTACAT CGCCAAGTGC CTCAAAGACC
1381ACAAGGCCAC CAGTTCCAAG GGGAGTGAAT GGAAGAAGGT GGCGAAAGTC ATAGACCGAT
1441TCTTCATGTG GATTTTTTTC ATTATGGTGT TTGTGATGAC TATTTTGATC ATAGCAAGAG
1501CGGATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1561TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1621CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTACTTAGT CCGCCGTTTA ACTCAACGCG
1681TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)