Construct: ORF TRCN0000466142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016506.1_s317c1
- Derived from:
- ccsbBroadEn_01878
- DNA Barcode:
- ACCGAAGAATAATCGTTTGCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBL4A (8266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8266 | UBL4A | ubiquitin like 4A | NM_014235.5 | 100% | 100% | |
| 2 | mouse | 100169864 | Gm44504 | predicted readthrough trans... | NM_001278271.1 | 85.6% | 89.8% | (many diffs) |
| 3 | mouse | 100169864 | Gm44504 | predicted readthrough trans... | NM_001278272.1 | 85.6% | 89.8% | (many diffs) |
| 4 | mouse | 27643 | Ubl4a | ubiquitin-like 4A | NM_145405.2 | 85.6% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gctgacggtg aaggcgctgc agggccgcga gtgcagcctg caggtgccag 121 aggacgagct ggtgtccacg ctgaagcagc tggtctccga gaagctgaac gtccCAGTGC 181 GCCAGCAGCG GCTGCTGTTC AAGGGCAAGG CCCTGGCAGA TGGGAAACGA CTCTCGGATT 241 ATAGCATCGG GCCCAACTCC AAGCTCAACC TAGTGGTCAA ACCCCTGGAG AAGGTGCTAC 301 TAGAAGAAGG CGAGGCCCAG AGGCTGGCCG ACTCCCCACC CCCGCAGGTC TGGCAGCTGA 361 TCTCCAAAGT CTTGGCCCGC CACTTCAGTG CGGCAGATGC CAGCAGGGTC CTGGAACAGC 421 TACAGAGGGA TTACGAGAGG TCCCTGAGTC GCCTGACGCT GGACGACATC GAACGGTTGG 481 CCAGCCGCTT CCTGCACCCT GAAGTGACTG AGACAATGGA GAAGGGCTTC TCCAAATACC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAACCGAAGA ATAATCGTTT GCGATCACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt