Construct: ORF TRCN0000466159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001284.1_s317c1
- Derived from:
- ccsbBroadEn_12057
- DNA Barcode:
- CAACCTATACCTGGAGATCATGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTMR12 (54545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54545 | MTMR12 | myotubularin related protei... | NM_001294343.2 | 100% | 100% | |
| 2 | human | 54545 | MTMR12 | myotubularin related protei... | NM_001040446.3 | 92.7% | 92.7% | 1513_1674del |
| 3 | human | 54545 | MTMR12 | myotubularin related protei... | NM_001294344.2 | 91.9% | 91.9% | 1343_1344ins168 |
| 4 | mouse | 268783 | Mtmr12 | myotubularin related protei... | NM_172958.3 | 80.8% | 83.2% | (many diffs) |
| 5 | mouse | 268783 | Mtmr12 | myotubularin related protei... | XM_017316630.1 | 62.8% | 64.1% | (many diffs) |
| 6 | mouse | 268783 | Mtmr12 | myotubularin related protei... | XM_006520071.2 | 57.2% | 59.5% | (many diffs) |
| 7 | mouse | 268783 | Mtmr12 | myotubularin related protei... | XR_001781539.1 | 39.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2145
- ORF length:
- 2079
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggggaaagga gtagtcggcg gtggcggcgg caccaaggcc cccaagccct 121 ccttcgtgtc gtacgtacgc cctgaggaaa ttcacacaaa cgaaaaggaa gtaacagaga 181 aggaagtaac tcttcacttg ttgccaggtg aacagctgct ttgtgaagcc agcacagtac 241 tgaagtatgt ccaggaagat tcctgtcagc atggggtcta tgggaggctt gtctgcacag 301 acttcaagat tgccttcttg ggtgatgatg aatctgcatt ggataatgat gaaactcaat 361 ttaagaataa ggttatagga gaaaatgaca ttacactcca ctgtgttgat cagatttatg 421 gagtgtttga tgagaaaaag aaaactctct ttggacaact gaagaaatac cctgagaagc 481 tcatcatcca ctgcaaagac cttcgagtgt tccagttttg tctgaggtac acaaaggaag 541 aggaagtcaa aaggattgtc agtggcataa ttcatcatac ccaggctcct aaactgctta 601 aacgattatt tctgttttcc tatgcgactg ctgcacaaaa caatacagtc actgatccca 661 agaaccatac cgtaatgttt gacacactta aggactggtg ttgggaactg gaacggacca 721 aaggcaacat gaagtacaaa gcagtgagtg tcaacgaagg ctataaagtc tgtgagagat 781 tgccagcata ctttgttgtc cccacccctc ttcctgaaga gaatgtgcag cgctttcagg 841 gtcatggcat accaatatgg tgttggtcct gccacaatgg aagtgccctt ttgaaaatgt 901 cagcactgcc caaagaacag gatgacggca ttttacaaat ccaaaagagc ttcttagatg 961 gaatttacaa gaccatccac aggccaccct atgaaattgt taaaacggaa gacctgtcaa 1021 gcaacttcct gtccctgcag gaaatccaga ctgcatactc taaatttaaa cagctatttc 1081 tgatagataa cagtactgaa ttttgggaca cagatataaa atggttttct ctgttggaaa 1141 gtagcagctg gcttgacata atcagacgtt gcctgaaaaa agcaatagag attacagaat 1201 gtatggaagc acaaaacatg aatgttcttc ttttagagga gaatgcatcc gacctctgct 1261 gtctcatttc ctctctggtg caactgatga tggaccccca ctgcagaacc agaattggtt 1321 tccagagcct catccaaaag gagtgggtca tgggtggcca ctgtttcttg gatcgctgca 1381 accatctccg ccagaacgac aaagaggagg ttcctgtgtt cctgcttttc ctagattgtg 1441 tctggcagct ggtgcaccag catcccccgg catttgaatt cacagagact tacctgactg 1501 ttttgtcaga tagcctgtat atacctattt ttagcacctt cttcttcaat tcacctcatc 1561 aaaaagatac taacatgcat caacgacaac tttctttgcc acttacacaa tctaagtcat 1621 ctcccaaaag aggatttttc agggaagaaa cagatcattt aattaaaaac cttctgggca 1681 agagaattag caaacttatt aattcttctg atgagctcCA AGACAACTTT CGAGAGTTCT 1741 ATGACAGCTG GCATAGCAAG TCCACTGACT ACCATGGTTT GTTGTTACCG CATATCGAGG 1801 GGCCCGAAAT CAAAGTCTGG GCCCAGCGCT ACCTACGTTG GATTCCAGAA GCCCAAATCC 1861 TAGGTGGTGG CCAAGTGGCC ACTCTGAGCA AACTCTTGGA AATGATGGAG GAAGTCCAGA 1921 GTTTACAAGA GAAGATCGAC GAGAGACACC ACAGCCAGCA GGCCCCCCAG GCTGAGGCCC 1981 CCTGCCTGCT GAGGAACTCT GCCCGCCTCT CGTCTTTGTT TCCTTTCGCT CTGCTCCAGC 2041 GACATTCCTC TAAGCCCGTC TTACCCACCA GTGGCTGGAA AGCTTTGGGA GATGAAGACG 2101 ATTTGGCCAA ACGAGAAGAT GAGTTCGTGG ACCTAGGGGA TGTGTGCCCA ACTTTCTTGT 2161 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2221 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2281 GAAAGGACGA CAACCTATAC CTGGAGATCA TGTTACGCGT TAAGTCgaca atcaacctct 2341 ggattacaaa atttgtgaaa gatt